Phylogenetic diversity of soda lake alkaliphiles

Duckworth, A. W.

doi:10.1016/0168-6496(96)00003-7

Cited by 43 publications

(46 citation statements)

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“…During the past few years, however, these environments have received more attention because of industrial needs for microbial enzymes from alkaliphilic bacteria, represented mainly by the alkaliphilic nonhalophilic Bacillus and halotolerant Halomonas species (Horikoshi 1996;Duckworth et al 1996). Recently, investigation of bacteria important in biogeochemical cycling in soda lakes has begun (Zavarzin 1993).…”

Section: Introductionmentioning

confidence: 99%

A new facultatively autotrophic hydrogen- and sulfur-oxidizing bacterium from an alkaline environment

Sorokin¹,

Tourova²,

Kuenen³

2000

Extremophiles

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An alkaliphilic bacterium, strain AHO 1, was isolated from an enrichment culture with hydrogen at pH 10 inoculated with a composite sample of sediments from five highly alkaline soda lakes (Kenya). This bacterium is a gram-negative, nonmotile, rod-shaped, obligately aerobic, and facultatively autotrophic hydrogen-oxidizing organism. It was able to oxidize reduced sulfur compounds to sulfate during heterotrophic growth. It utilized a wide range of organic compounds as carbon and energy sources and grew mixotrophically with hydrogen and acetate. With sulfur compounds, mixotrophic growth was observed only in acetate-limited continuous culture. The normal pH range for autotrophic growth with hydrogen was pH 8.0-10.25, with a pH optimum at 9-9.5. Growth at pH values lower than 8.0 was extremely slow. Heterotrophic growth with acetate was optimal at pH 10.0. The hydrogen-oxidizing activity of whole cells was maximal at pH 9.0 and still substantial up to pH 11. NAD-dependent hydrogenase activity was found in the soluble fraction of the cell-free extract, but no methylene blue-dependent activity in either the soluble or membrane fractions was observed. On the basis of its pH profile, the soluble hydrogenase of strain AHO 1 was a typical pH-neutral enzyme. Phylogenetic analysis revealed that strain AHO 1 belongs to the alpha-3 subgroup of the Proteobacteria with a closest relation to a recently described alkaliphilic aerobic bacteriochlorophyll a-containing bacterium "Roseinatronobacter thiooxidans."

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Section: Introductionmentioning

confidence: 99%

A new facultatively autotrophic hydrogen- and sulfur-oxidizing bacterium from an alkaline environment

Sorokin¹,

Tourova²,

Kuenen³

2000

Extremophiles

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“…Some strains have been assigned to a new species, Stenotrophomonas nitritireducens (Finkmann et al, 2000) (see note at the end of the list of species). From a soda lake, an alkaliphilic strain was studied by Duckworth et al (1996).…”

Section: Pathogenicity For Humansmentioning

confidence: 99%

“…These organisms may represent a very high proportion of the non-fermentative, Gram-negative bacteria in the soil microflora (Debette and Blondeau, 1977), and they have been isolated in large numbers from manured soils in Japan (Katoh and Suzuki, 1979) (cited by Ikemoto et al, 1980). The isolation of three strains similar to S. maltophilia, from gut and feces of the arthropod Folsomia candida was reported by Duckworth et al (1996).…”

Section: Pathogenicity For Humansmentioning

confidence: 99%

Stenotrophomonas

Palleroni

2015

Bergey's Manual of Systematics of Archaea and Bacteria

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Ste.no.tro.pho.mo' nas . Gr. adj. stenus narrow; Gr. n. trophus one who feeds; Gr. n. monas a unit, monad; M.L. fem. n. Stenotrophomonas a unit feeding on few substrates. Proteobacteria / Gammaproteobacteria / Xanthomonadales / Xanthomonadaceae / Stenotrophomonas Straight or curved rods but not helical, 0.5 × 1.5 µm, occur singly or in pairs. Do not accumulate poly‐β‐hydroxybutyrate granules as intracellular reserve material. No resting stages are known. Gram negative. Motile by two or more polar flagella. Aerobic, having a strictly respiratory type of metabolism with oxygen as electron acceptor. Nitrate is reduced but it is not used as a nitrogen source for growth . The colonies are yellow, greenish, or gray. The yellow color is not due to carotenoids or xanthomonadins. The colonies may turn a dark brown color with age. The major polyamines are spermidine and cadaverine . Aside from fatty acids in common with Xanthomonas species, one of the species ( S . maltophilia ) is characterized by the presence of C 17 : 0 cyclopropane fatty acid and ubiquinone Q8. No growth occurs at 4 or 41°C; the optimum growth temperature is ~35°C. Oxidase negative . Gelatinase and catalase positive. Strong lipolytic activity, as judged by the hydrolysis of Tween 80. Chemoorganotrophic . One of the species ( S . maltophilia ) is widely distributed in nature and is commonly isolated from clinical materials and nosocomial infections. The mol % G + C of the DNA is : 66.9 ± 0.8. Type species : Stenotrophomonas maltophilia (Hugh 1981) Palleroni and Bradbury 1993, 608 ( Pseudomonas maltophilia Hugh 1981, 195; Xanthomonas maltophilia Swings, De Vos, Van Den Mooter and De Ley 1983, 412.)

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“…The 16S rRNA gene sequence was amplified as described by Duckworth et al (1996). The 16S rRNA gene sequences of the isolates were a continuous stretch (1468, 1439 bp) and analyzed according to the methods described previously (Liu et al 2010).…”

Section: Nuclear Dna Isolation and Phylogenetic Analysesmentioning

confidence: 99%

Halomonas aidingensis sp. nov., a moderately halophilic bacterium isolated from Aiding salt lake in Xinjiang, China

Liu

Wang

Yuan

2010

Antonie van Leeuwenhoek

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Two Gram-negative moderately halophilic bacterial strains, designated Ad-1(T) and C-12, were isolated from Aiding salt lake of Xinjiang in China. The novel isolates were subjected to a polyphasic taxonomic study. Cells of these strains were cocci or short rods and motile with polar flagella. Colonies produced brown-red pigment. The isolates grew in the range of 0.5-25% (w/v) NaCl, pH 5.5-10.5 and 4-45°C. Analysis of their 16S rRNA gene sequences indicated that strains Ad-1(T) and C-12 belonged to the genus Halomonas showing 92.7-98.4% similarity with the type species. The isoprenoid quinones of the isolates were Q-9 and Q-8. The major cellular fatty acids were C18:1ω7c, C16:1ω7c/6c, C16:0, C12:0-3OH and C10:0. The DNA G + C contents of strains Ad-1(T) and C-12 were 64.6 and 63.9 mol%, respectively. The DNA relatedness between the two isolates was 89.2%. The similarities of these newly isolated strains with closely related type strains were lower than 35% at the genetic level. Based on phenotypic, chemotaxonomic and genetic characteristics, the representative strain Ad-1(T) is considered to be a novel species of the genus Halomonas, for which the name Halomonas aidingensis sp. nov. is proposed, with Ad-1(T) (= CGMCC 1.10191(T) = NBRC 106173(T)) as the type strain.

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Phylogenetic diversity of soda lake alkaliphiles

Cited by 43 publications

References 0 publications

A new facultatively autotrophic hydrogen- and sulfur-oxidizing bacterium from an alkaline environment

A new facultatively autotrophic hydrogen- and sulfur-oxidizing bacterium from an alkaline environment

Stenotrophomonas

Halomonas aidingensis sp. nov., a moderately halophilic bacterium isolated from Aiding salt lake in Xinjiang, China

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