Phylogenetic Diversity of Microorganisms from the Sludge of a Biogas Reactor Processing Oil-Containing and Municipal Waste

Назина, Т. Н.; Соколова, Д. Ш.; Бабич, Т. Л.; Семенова, Е. М.; Borzenkov, I. A.; Bidzhieva, S. Kh.; Merkel, Alexander Y.; Khisametdinov, M. R.; Tourova, T. P.

doi:10.1134/s0026261718030074

Cited by 16 publications

(6 citation statements)

References 25 publications

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“…Candidatus Cloacimonetes was found to be highly reduced during the disturbance phase. It has previously been reported to be present in high abundance in high-ammonia systems and is suggested to have a specialist function (syntrophic propionateoxidation) 39,105 and to be a potential acetogenic candidate 104,[106][107][108][109][110][111][112][113][114][115] . In line with the results in the present study, a decrease abundance in the phylum Cloacimonetes in association with process disturbance has been seen previously Klang et al 104 .…”

Section: Discussionmentioning

confidence: 99%

Profiling temporal dynamics of acetogenic communities in anaerobic digesters using next-generation sequencing and T-RFLP

Singh

Müller

Schnürer

2021

Sci Rep

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Acetogens play a key role in anaerobic degradation of organic material and in maintaining biogas process efficiency. Profiling this community and its temporal changes can help evaluate process stability and function, especially under disturbance/stress conditions, and avoid complete process failure. The formyltetrahydrofolate synthetase (FTHFS) gene can be used as a marker for acetogenic community profiling in diverse environments. In this study, we developed a new high-throughput FTHFS gene sequencing method for acetogenic community profiling and compared it with conventional terminal restriction fragment length polymorphism of the FTHFS gene, 16S rRNA gene-based profiling of the whole bacterial community, and indirect analysis via 16S rRNA profiling of the FTHFS gene-harbouring community. Analyses and method comparisons were made using samples from two laboratory-scale biogas processes, one operated under stable control and one exposed to controlled overloading disturbance. Comparative analysis revealed satisfactory detection of the bacterial community and its changes for all methods, but with some differences in resolution and taxonomic identification. FTHFS gene sequencing was found to be the most suitable and reliable method to study acetogenic communities. These results pave the way for community profiling in various biogas processes and in other environments where the dynamics of acetogenic bacteria have not been well studied.

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Section: Discussionmentioning

confidence: 99%

Profiling temporal dynamics of acetogenic communities in anaerobic digesters using next-generation sequencing and T-RFLP

Singh

Müller

Schnürer

2021

Sci Rep

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“…Clone NB1-n (phylum Tenericutes) has been found in aquatic sediments [ 85 , 86 , 87 ], rumen [ 85 ], a methanogenic AD process treating oil-containing municipal waste [ 88 ], and cellulose-rich substrate [ 89 ]. According to the recovered genome, there is no direct proof that NB1-n is involved in methane metabolic pathways, but it may have metabolic potential for fermenting simple sugars and producing small organic acids, such as acetic acids [ 85 ].…”

Section: Discussionmentioning

confidence: 99%

Diversity and Abundance of Microbial Communities in UASB Reactors during Methane Production from Hydrolyzed Wheat Straw and Lucerne

et al. 2020

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The use of straw for biofuel production is encouraged by the European Union. A previous study showed the feasibility of producing biomethane in upflow anaerobic sludge blanket (UASB) reactors using hydrolyzed, steam-pretreated wheat straw, before and after dark fermentation with Caldicellulosiruptor saccharolyticus, and lucerne. This study provides information on overall microbial community development in those UASB processes and changes related to acidification. The bacterial and archaeal community in granular samples was analyzed using high-throughput amplicon sequencing. Anaerobic digestion model no. 1 (ADM1) was used to predict the abundance of microbial functional groups. The sequencing results showed decreased richness and diversity in the microbial community, and decreased relative abundance of bacteria in relation to archaea, after process acidification. Canonical correspondence analysis showed significant negative correlations between the concentration of organic acids and three phyla, and positive correlations with seven phyla. Organic loading rate and total COD fed also showed significant correlations with microbial community structure, which changed over time. ADM1 predicted a decrease in acetate degraders after a decrease to pH ≤ 6.5. Acidification had a sustained effect on the microbial community and process performance.

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“…MBA03 was first reported in a thermophilic process treating municipal solid waste (Tang et al, 2004). MBA03 has thereafter been detected in various thermophilic AD, fed for example with cattle manure and steam-exploded wheat straw (Sun et al, 2015), swine manure (Wu et al, 2020), municipal solid waste (Nazina et al, 2018), or food waste under high solid conditions (Westerholm et al, 2020). MBA03 has also been shown to positively respond to trace element addition in monodigestion of grass silage (FitzGerald et al, 2019).…”

Section: Temperature Change and Response In The Bacterial Communitymentioning

confidence: 99%

Anaerobic Digestion of Animal Manure and Influence of Organic Loading Rate and Temperature on Process Performance, Microbiology, and Methane Emission From Digestates

Ahlberg-Eliasson¹,

Westerholm²,

Isaksson³

et al. 2021

Front. Energy Res.

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Biogas production from manure is of particular value in regard of lowering greenhouse gas emissions and enhancing nutrient re-circulation. However, the relatively low energy content and the characteristics of manure often result in low degradation efficiency, and the development of operating strategies is required to improve the biogas yield and the economic benefits. In this study, the potential to enhance the performance of two full-scale biogas plants operating with cattle manure, in mono-digestion or combined with poultry manure, was investigated. Four continuously fed laboratory-scale reactors were operated in sets of two, in which the temperature in one reactor in each set was increased from 37–42°C to 52°C. The potential to increase the capacity was thereafter assessed by increasing the organic loading rate (OLR), from ca 3 to 5 kg volatile solids (VS)/ m3 and day. The processes were evaluated with both chemical and microbiological parameters, and in addition, the residual methane potential (RMP) was measured to evaluate the risk of increased methane emissions from the digestate. The results showed that both processes could be changed from mesophilic to themophilic temperature without major problems and with a similar shift in the microbial community profile to a typical thermophilic community, e.g., an increase in the relative abundance of the phylum Firmicutes. However, the temperature increase in the reactor co-digesting cattle and poultry manure caused a slight accumulation of fatty acids (2 g/l) and reduced the specific methane production, most likely due to ammonia inhibition (0.4–0.7 g NH3/l). Still, during operation at higher OLR, thermophilic as compared to mesophilic temperature slightly increased the methane yield and specific methane production, in both investigated processes. However, the higher OLR decreased the overall degree of degradation in all processes, and this showed a positive correlation with increased RMP values. Chemical analyses suggested that high RMP values (40–98 Nml gVS−1) were related to the degradation of cellulose, hemicellulose, and volatile fatty acid enriched in the digestate. Conclusively, increased temperature and load can increase the methane yield from manure but can result in less efficient degradation and increased risks for methane emissions during storage and handling of the digestate.

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Phylogenetic Diversity of Microorganisms from the Sludge of a Biogas Reactor Processing Oil-Containing and Municipal Waste

Cited by 16 publications

References 25 publications

Profiling temporal dynamics of acetogenic communities in anaerobic digesters using next-generation sequencing and T-RFLP

Profiling temporal dynamics of acetogenic communities in anaerobic digesters using next-generation sequencing and T-RFLP

Diversity and Abundance of Microbial Communities in UASB Reactors during Methane Production from Hydrolyzed Wheat Straw and Lucerne

Anaerobic Digestion of Animal Manure and Influence of Organic Loading Rate and Temperature on Process Performance, Microbiology, and Methane Emission From Digestates

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