Phylogenetic diversity of microorganisms associated with the deep-water sponge Baikalospongia intermedia

Kaluzhnaya, O. V.; Ицкович, В. Б.

doi:10.1134/s1022795414060052

Cited by 17 publications

(9 citation statements)

References 39 publications

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“…from the southern Atlantic 36 . In addition, they were also found in the Antarctic soft coral Alcyonium antarcticum 37 , ascidians of the Great Barrier Reef 38 , and the deep lake water sponge Baikalospongia intermedia 39 . These findings suggest that green sulfur bacteria are prevalent in aquatic animals, and might play an important role in the host.…”

Section: Resultsmentioning

confidence: 95%

Metagenomic analysis reveals a green sulfur bacterium as a potential coral symbiont

Lin

Zhou

Tian

et al. 2017

Sci Rep

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Coral reefs are ecologically significant habitats. Coral-algal symbiosis confers ecological success on coral reefs and coral-microbial symbiosis is also vital to coral reefs. However, current understanding of coral-microbial symbiosis on a genomic scale is largely unknown. Here we report a potential microbial symbiont in corals revealed by metagenomics-based genomic study. Microbial cells in coral were enriched for metagenomic analysis and a high-quality draft genome of “Candidatus Prosthecochloris korallensis” was recovered by metagenome assembly and genome binning. Phylogenetic analysis shows “Ca. P. korallensis” belongs to the Prosthecochloris clade and is clustered with two Prosthecochloris clones derived from Caribbean corals. Genomic analysis reveals “Ca. P. korallensis” has potentially important ecological functions including anoxygenic photosynthesis, carbon fixation via the reductive tricarboxylic acid (rTCA) cycle, nitrogen fixation, and sulfur oxidization. Core metabolic pathway analysis suggests “Ca. P. korallensis” is a green sulfur bacterium capable of photoautotrophy or mixotrophy. Potential host-microbial interaction reveals a symbiotic relationship: “Ca. P. korallensis” might provide organic and nitrogenous nutrients to its host and detoxify sulfide for the host; the host might provide “Ca. P. korallensis” with an anaerobic environment for survival, carbon dioxide and acetate for growth, and hydrogen sulfide as an electron donor for photosynthesis.

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Section: Resultsmentioning

confidence: 95%

Metagenomic analysis reveals a green sulfur bacterium as a potential coral symbiont

Lin

Zhou

Tian

et al. 2017

Sci Rep

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“…For the first time, we performed a comparative analysis of diseased and healthy B. intermedia; we identified a decrease in Chlorophyta and an increase in Bacteroidetes and Cyanobacteria in diseased specimen. Until now, very few studies have been conducted on the microbiome in B. intermedia [21,24,26]. Proteobacteria, Actinobacteria, Planctomycetes, Cloroflexi, Verrucomicrobia, Acidobacteria, Chlorobi and Nitrospirae were identified within the B. intermedia microbiome [21].…”

Section: Discussionmentioning

confidence: 99%

“…Most research has been conducted on the microbial associations of marine sponges. Symbiotic associations of freshwater sponges have been insufficiently studied, even though these sponges also have diverse microbiomes including Actinobacteria, alpha-, beta-proteobacteria, Verrucomicrobia, and Flavobacterium [20][21][22][23][24][25][26].…”

Section: Introductionmentioning

confidence: 99%

Microbiome Changes of Endemic Lake Baikal Sponges during Bleaching Syndrome Development

et al. 2021

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The sponge (Porifera) microbiome is an indicator of both natural and anthropogenic stressors. Studying Baikal sponge microbial communities could help reveal if there is a connection between bacterial symbionts and a mass sponge bleaching event that was recently detected; 16S rRNA sequencing was performed among healthy and diseased freshwater sponges of Lubomirskia baikalensis and Baikalospongia intermedia, which were collected from Lake Baikal, Russia. A phylum-based taxonomic classification showed that Chlorophyta, Acidobacteria, Bacteroidetes, Actinobacteria and Cyanobacteria were most abundant across samples. When comparing healthy and diseased L. baikalensis samples, large variations in microbial composition were found at the phylum level. Comparative analyses, which were performed for the first time for B. intermedia, showed a decrease in Chlorophyta (unicellular green algae) and an increase in Bacteroidetes and Cyanobacteria in diseased specimens. At the genus level, the Opitutus (Verrucomicrobia), Planctomyces, and Nitrospira content increased in all diseased sponges, which reflected a general tendency toward an increase in Cyanobacteria in diseased sponges. Comparative analysis of the diseased and healthy sponge metagenomes showed that diseased sponges underwent various nonspecific changes in bacterial composition. The bacterial community composition is probably influenced by sponge type and degree of disease affection.

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“…(Manivasagan et al, 2014;Lee et al, 2020). Actinobacteria were found in all previously studied communities of freshwater sponges, constituting a significant part of the bacterial 16S rRNA sequences in them (Kaluzhnaya et al, 2011;2012;Kaluzhnaya and Itskovich, 2014;Gladkikh et al, 2014;Seo et al, 2016;Kulakova et al, 2018).…”

Section: Discussionmentioning

confidence: 97%

“…Proteobacteria is a very heterogeneous group, which includes both symbionts of eukaryotes and a large number of pathogenic and opportunistic microorganisms, photoand chemotrophic species of bacteria, autotrophs and heterotrophs. Alphaproteobacteria, as a rule, dominate proteobacteria in freshwater sponge microbiomes (Gernert et al, 2005;Costa et al, 2013;Kaluzhnaya et al, 2011;2012;Kaluzhnaya and Itskovich, 2014). Among Alphaproteobacteria, some phylotypes specific for communities of freshwater sponges have been identified, which indicates the possible co-evolution of sponges and some representatives of symbiotic bacteria as well as the existence of vertical transfer of symbiotic microorganisms (Taylor et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

PCR-screening of bacterial strains isolated from the microbiome of the Lubomirskia baicalensis sponge for the presence of secondary metabolite synthesis genes

Kaluzhnaya

Lipko

Ицкович

2021

LFWB

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Abstract. The microbial communities of sponges (Porifera) are often a source of natural bioactive metabolites. From the microbiome of the endemic Lubomirskia baicalensis sponge, 35 bacterial strains were isolated and identified using molecular methods. The strains belonged to the phyla Actinobacteria, Firmicutes, and Proteobacteria (classes Alpha- and Betapriteobacteria). To analyze the strains for the presence of genes in the synthesis of secondary metabolites, polyketide synthases (PKS), PCR screening was applied using degenerate primers. Overall, 15 out of 35 strains contained PCR products corresponding in size to a fragment of the ketosynthase domain of the PKS gene cluster. Thus, the proposed method is applicable for rapid screening of the potential ability of microorganisms of different taxonomic groups to produce secondary metabolites. The work contributes to the study of the taxonomic diversity of cultured microorganisms, potential producers of biologically active substances, isolated from the microbiomes of Baikal sponges.

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Phylogenetic diversity of microorganisms associated with the deep-water sponge Baikalospongia intermedia

Cited by 17 publications

References 39 publications

Metagenomic analysis reveals a green sulfur bacterium as a potential coral symbiont

Metagenomic analysis reveals a green sulfur bacterium as a potential coral symbiont

Microbiome Changes of Endemic Lake Baikal Sponges during Bleaching Syndrome Development

PCR-screening of bacterial strains isolated from the microbiome of the Lubomirskia baicalensis sponge for the presence of secondary metabolite synthesis genes

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