Phylogenetic diversity of isolates belonging to genera Geobacillus and Aeribacillus isolated from different geothermal regions of Turkey

Abstract

The phylogenetic diversity of 31 thermophilic bacilli belonging to genera Geobacillus and Aeribacillus were investigated which were isolated from various geothermal sites of Turkey. Twenty-seven of these isolates were found to be belonged within the genus Geobacillus, whereas 4 of them were identified as Aeribacillus pallidus. The comparative 16S rRNA gene sequence analyses revealed that the A. pallidus isolates displayed sequence similarity values from 98.0 to 99.6% to their closest relative. Furthermore, Geobacillus isolates showed sequence similarity values from 88.9 to 99.8% with the reference type strains. According to the phylogenetic analysis, isolates belonging to genus Geobacillus were diverged into Moreover, the sequence comparisons of E173a, E265, C161ab and A142 isolates demonstrated that they represented novel species among genus Geobacillus as they shared lower than 96.7% sequence similarity to all the described type species. The AluI-, HaeIII-and TaqI-AR-DRA results were in congruence with the 16S rRNA gene sequence analyses. By ARDRA results, the isolates were able to be differentiated and clustered, the discriminative restriction fragments of these isolates and type species were determined and the novelty of E173, E265, C161ab and A142 isolates could be displayed. Some differentiating phenotypic characters and the ability of amylase, glucosidase and protease production of these bacilli were also studied and biotechnologically valuable thermostable enzyme producing isolates were introduced in order to use in further studies.

Culture dependent diversity and phylogeny of thermophilic bacilli from a natural hot spring reservoir in the Gir Forest, Gujarat (India)

“…The bacterial isolates were Gram positive, displaying varia tion in their cell morphology, cell size and arrange ments (Table 1). Similar studies have been reported on the culture dependent diversity by Cihan et al (2012), where the bacterial isolates, belonging to the genus Geobacillus, grew between 50 and 70°C and also dis played Gram positive or Gram variable features, endospore formation, catalase activity and motility (Cihan et al, 2012).…”

“…This approach includes the phenotypic and genotypic information, based on various molecular fingerprint ing methods to generate various clusters of the bacte ria, belonging to the same genus (Vaneechoutte et al, 1992;Cihan et al, 2012;. Presently, RFLP 16S rDNA is recognized as a powerful and rapid method to identify relatives of a large number of the newer isolates (Vinuesa et al, 2005); (Appunu et al, 2008).…”

“…However, as per the In Silico ARDRA patterns, its position in the restriction map (5' to 3' direction) var ied considerably, which was used to further diversify the bacterial community in hot spring at the Tulsi Shyam, India (Table 4). Similarly, Cihan et al (2012) compared the experimental and theoretical ARDRA profiles, for judging the diversity of Geobacillus sp. and Aeribacillus sp., using the restriction enzymes Hae III, Alu I and Taq I.…”

“…The emerging profiles are used either to cluster the community into genotypic groups or for the strain typing. Reportedly, ARDRA was used in the analysis of the mixed bacterial popula tions, belonging to various environmental niches (Cihan et al, 2012;. Consequently, the polyphasic approaches are gaining attention in the field of microbial ecology to screen the microbes pro ducing novel enzymes, having unique properties for the newer applications (Derekova et al, 2008;Kikani et al, 2010;.…”

Unraveling the Lipolytic Activity of Thermophilic Bacteria Isolated from a Volcanic Environment

“…This high 16S rDNA similarity is characteristic of this relatively recently characterized genus [37] that includes species with promising biotechnological potential [38]. Members of geobacilli have been reported to represent the majority of the species that inhabit a variety of diverse “hot” environments such as marine thermal vents [39, 40], high temperature oil fields [37], hot springs [41], geothermal regions [42], and sugar refinery wastewaters [43]. …”