Phylogenetic diversity, antibiotic resistance and virulence traits of Aeromonas spp. from untreated waters for human consumption

Carvalho, Maria J.; Martínez‐Murcia, Antonio; Esteves, Ana Cristina; Saavedra, María José

doi:10.1016/j.ijfoodmicro.2012.09.008

Cited by 56 publications

(45 citation statements)

References 47 publications

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“…The widespread occurrence of Aeromonas ssp. in fish and seafood, vegetables, meat, poultry, raw milk, and different water sources has been confirmed by others (Villari et al, 2000; Kingombe et al, 2004; Heaton and Jones, 2008; Xanthopoulos et al, 2010; Ottaviani et al, 2011; Carvalho et al, 2012; Latif-Eugenín et al, 2016). …”

Section: Introductionsupporting

confidence: 59%

Species Distribution and Prevalence of Putative Virulence Factors in Mesophilic Aeromonas spp. Isolated from Fresh Retail Sushi

2017

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Aeromonas spp. are ubiquitous bacteria that have received increasing attention as human pathogens because of their widespread occurrence in food, especially seafood and vegetables. The aim of this work was to assess the species identity and phylogenetic relationship of 118 Aeromonas strains isolated from fresh retail sushi from three producers, and to characterize the isolates with respect to genetic and phenotypic virulence factors. We also evaluate the potential hazard associated with their presence in ready-to-eat seafood not subjected to heat treatment. Mesophilic Aeromonas salmonicida was most prevalent (74%), followed by A. bestiarum (9%), A. dhakensis (5%), A. caviae (5%), A. media (4%), A. hydrophila (2%), and A. piscicola (1%). All isolates were considered potentially pathogenic due to the high prevalence of genes encoding hemolysin (hlyA) (99%), aerolysin (aerA) (98%), cytotoxic enterotoxin (act) (86%), heat-labile cytotonic enterotoxin (alt) (99%), and heat-stable cytotonic enterotoxin (ast) (31%). The shiga-like toxins 1 and 2 (stx-1 and stx-2) were not detected. Moreover, there was heterogeneity in toxin gene distribution among the isolates, and the combination of act/alt/hlyA/aerA was most commonly detected (63%). β-hemolysis was species-dependent and observed in 91% of the isolates. All A. media and A. caviae strains were non-hemolytic. For isolates belonging to this group, lack of hemolysis was possibly related to the absence of the act gene. Swimming motility, linked to adhesion and host invasion, occurred in 65% of the isolates. Partial sequencing of the gyrB gene demonstrated its suitability as a genetic marker for Aeromonas species identification and for assessment of the phylogenetic relationship between the isolates. The gyrB sequence divergence within a given species ranged from 1.3 to 2.9%. A. bestiarum, A. salmonicida, and A. piscicola were the most closely related species; their sequences differed by 2.7–3.4%. The average gyrB sequence similarity between all species was 93%, demonstrating its acceptable taxonomic resolution. The presence of multiple species of potential pathogenic Aeromonas in fresh retail sushi raises new food safety issues related to the increased consumption of ready-to-eat food composed of raw ingredients.

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Section: Introductionsupporting

confidence: 59%

Species Distribution and Prevalence of Putative Virulence Factors in Mesophilic Aeromonas spp. Isolated from Fresh Retail Sushi

2017

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“…(Carvalho et al, 2012;Henriques et al, 2006;Lee et al, 2008;Moura et al, 2012). In this study, the majority of ESBLproducing isolates harboured intI1 gene, while the incidence of detection of the class 2 integrons was considerably lower.…”

Section: Discussionmentioning

confidence: 45%

“…Recently, the most disseminated ESBLs among Gram-negative bacteria worldwide, the CTX-M ESBLs (Cantón et al, 2012), have been identified in clinical isolates of Aeromonas, with CTX-M-15 detected in A. hydrophila (Gómez-Garcés et al, 2011) and CTX-M-3 in A. caviae . In addition, several Aeromonas species, among which A. hydrophila, have been found to carry integrons encoding co-resistance to tetracyclines, aminoglycosides, chloramphenicol and trimethoprim (Carvalho et al, 2012;Kadlec et al, 2011;Moura et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Aeromonas spp. simultaneously harbouring blaCTX-M-15, blaSHV-12, blaPER-1 and blaFOX-2, in wild-growing Mediterranean mussel (Mytilus galloprovincialis) from Adriatic Sea, Croatia

Maravić

Skočibušić

Šamanić

et al. 2013

International Journal of Food Microbiology

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“…Freshwater constitute favourable living ecosystem for numerous organisms such as Aeromonas spp [1]. Water constitutes not only a way of dissemination of antibiotic-resistant organisms among human and animal population, as drinking water is produced from surface water, but also the route by which resistance genes are introduced in natural bacterial ecosystems.…”

Section: Introductionmentioning

confidence: 99%

Antibiotic Resistance Bacteria from Rivers Water in Algeria

Djenadi¹

2017

J Bacteriol Parasitol

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The aim of the current investigation was to look on the presence of the antibiotic resistance bacteria in natural environment in Algeria. From fresh water, multidrug resistant bacteria were harvested, and then investigated for their resistance profile and extend spectrum Beta lactamase and metallo Beta lactamase production. From isolate bacteria only Aeromonas hydrophila catch our attention, because of their metallo Beta lactamase production and alimentary and clinical impact. This finding confirmed our hypothesis that natural environment could be colonized by multidrug resistant bacteria especially to carbapenem antibiotic who may be induced by mutation or horizontal gene transfer. The spread of these kinds of resistance organisms may become a serious issue for the public health and food security.

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Phylogenetic diversity, antibiotic resistance and virulence traits of Aeromonas spp. from untreated waters for human consumption

Cited by 56 publications

References 47 publications

Species Distribution and Prevalence of Putative Virulence Factors in Mesophilic Aeromonas spp. Isolated from Fresh Retail Sushi

Species Distribution and Prevalence of Putative Virulence Factors in Mesophilic Aeromonas spp. Isolated from Fresh Retail Sushi

Aeromonas spp. simultaneously harbouring blaCTX-M-15, blaSHV-12, blaPER-1 and blaFOX-2, in wild-growing Mediterranean mussel (Mytilus galloprovincialis) from Adriatic Sea, Croatia

Antibiotic Resistance Bacteria from Rivers Water in Algeria

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