Phylogenetic Diversity and Characterization of Novel and Efficient Cellulase Producing Bacterial Isolates from Various Extreme Environments

Pandey, Sangeeta; Singh, Surender; Yadav, Ajar Nath; Nain, Lata; Saxena, Anil Kumar

doi:10.1271/bbb.130121

Cited by 87 publications

(32 citation statements)

References 35 publications

(30 reference statements)

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“…Our present results were in support with the earlier study that demonstrated thermostable cellulase production at an optimum temperature of 50°C by Brevibacillus thermoruber (Derekova et al 2008). There are many reports on production of thermostable cellulase and xylanase from bacterial isolates from thermal springs/high temperature habitats (Gerasimova and Kuisiene 2012; Kumar et al 2014b;Pandey et al 2013;Suman et al 2015). In general the cellulases and xylanases are of special interest due to abundance of cellulose and hemicellulose in nature, moreover hydrolysis of these substrates require temperature tolerant/thermostable enzymes (Bajaj and Abbass 2011).…”

Section: Resultssupporting

confidence: 93%

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Hot springs of Indian Himalayas: potential sources of microbial diversity and thermostable hydrolytic enzymes

et al. 2017

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Microbial communities in hot springs at high elevations have been extensively studied worldwide. In this sense, the Indian Himalaya regions is valuable ecosystems for providing both the extreme 'cold' and 'hot' sites for exploring microbial diversity. In the present study, a total of 140 thermophilic bacteria were isolated from 12 samples collected from Manikaran and Yumthang hot springs of Indian Himalayas. The bacterial isolates were studied for phylogenetic profiling, growth properties at varying conditions and potential sources of extracellular thermostable hydrolytic enzymes such as protease, amylase, xylanase and cellulase. Based on production of extracellular hydrolases, 51 isolates from Manikaran (28) and Yumthang thermal springs (23) were selected and identified using 16S rRNA gene sequencing which included 37 distinct species of 14 different genera namely Anoxybacillus, Bacillus, Brevibacillus, Brevundimonas, Burkholderia, Geobacillus, Paenibacillus, Planococcus, Pseudomonas, Rhodanobacter, Thermoactinomyces, Thermobacillus, Thermonema and Thiobacillus. Out of 51 hydrolase producing bacteria, 24 isolates showed stability at wide range of temperature and pH treatments. In present investigation, three thermotolerant bacteria namely, Thermobacillus sp NBM6, Paenibacillus ehimensis NBM24 and Paenibacillus popilliae NBM68 were found to produced cellulase-free xylanase. These potential extracellular thermostable hydrolytic enzymes producing thermophilic bacteria have a great commercial prospect in various industrial, medical and agriculture applications.

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Section: Resultssupporting

confidence: 93%

“…Cellulase free xylanase are of paramount significance in some of industries (Adhyaru et al 2014;Pandey et al 2013). Treatment with xylanase at elevated temperature disrupts the cell wall structure, facilitates lignin removal in the various stages of bleaching of paper, Therefore xylanase must lack cellulolytic activity.…”

Section: Resultsmentioning

confidence: 99%

Hot springs of Indian Himalayas: potential sources of microbial diversity and thermostable hydrolytic enzymes

et al. 2017

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“…The amount of DNA extracted was assessed by electrophoresis on an 0.8 % agarose gel. The 16S rRNA gene was amplified as described earlier (Pandey et al 2013) using the universal primers pA (5′-AGAGTTTG ATCCTGGCTCAG-3′) and pH (5′-AAGGAGGTGATCCA GCCGCA-3′) (Edwards et al 1989). The PCR-amplified 16S rRNA gene was purified by a QIA quick PCR product purification kit (Qiagen, Hilden, Germany).…”

Section: Physico-chemical Properties Of Samplesmentioning

confidence: 99%

Assessment of genetic diversity and plant growth promoting attributes of psychrotolerant bacteria allied with wheat (Triticum aestivum) from the northern hills zone of India

et al. 2015

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The biodiversity of wheat-associated bacteria from the northern hills zone of India was deciphered. A total of 247 bacteria was isolated from five different sites. Analysis of these bacteria by amplified ribosomal DNA restriction analysis (ARDRA) using three restriction enzymes, AluI, MspI and HaeIII, led to the grouping of these isolates into 19-33 clusters for the different sites at 75 % similarity index. 16S rRNA gene based phylogenetic analysis revealed that 65 %, 26 %, 8 % and 1 % bacteria belonged to four phyla, namely Proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes, respectively. Overall, 28 % of the total morphotypes belonged to P s e u d o m o n a s followed by B a c i l l u s ( 2 0 % ) , Stenotrophomonas (9 %), Methylobacterium (8 %), Arthrobacter (7 %), Pantoea (4 %), Achromobacter, Acinetobacter, Exiguobacterium and Staphylococcus (3 %), Enterobacter, Providencia, Klebsiella and Leclercia (2 %), Brevundimonas, Flavobacterium, Kocuria, Kluyvera and Planococcus (1 %). Representative strains from each cluster were screened in vitro for plant growth promoting traits, which included solubilisation of phosphorus, potassium and zinc; production of ammonia, hydrogen cyanide, indole-3-acetic acid and siderophore; nitrogen fixation, 1-aminocyclopropane-1-carboxylate deaminase activity and biocontrol against Fusarium graminearum, Rhizoctonia solani and Macrophomina phaseolina. Cold-adapted isolates may have application as inoculants for plant growth promotion and biocontrol agents for crops growing under cold climatic conditions.

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“…Among Bacillus and Bacillus-derived genera, Bacillus thuringiensis, Bacillus subtilis, Bacillus pumilus, Bacillus mycoides, Bacillus megaterium, and Bacillus firmus are ubiquitous in rhizosphere saline soil [1,27,28]. The genus Bacillus consists of a heterogenic group of endospores forming Gram-positive rods, which survive for extended periods under adverse environmental conditions due to endospores.…”

Section: Biodiversity Of Halophilic Microbesmentioning

confidence: 99%

Biodiversity and biotechnological applications of halophilic microbes for sustainable agriculture

2018

J App Biol Biotech

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Phylogenetic Diversity and Characterization of Novel and Efficient Cellulase Producing Bacterial Isolates from Various Extreme Environments

Cited by 87 publications

References 35 publications

Hot springs of Indian Himalayas: potential sources of microbial diversity and thermostable hydrolytic enzymes

Hot springs of Indian Himalayas: potential sources of microbial diversity and thermostable hydrolytic enzymes

Assessment of genetic diversity and plant growth promoting attributes of psychrotolerant bacteria allied with wheat (Triticum aestivum) from the northern hills zone of India

Biodiversity and biotechnological applications of halophilic microbes for sustainable agriculture

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