Phylogenetic characterisation of bacterial symbionts in the accessory nidamental glands of the sepioid Sepia officinalis (Cephalopoda: Decapoda)

Grigioni, Sveva; Boucher-Rodoni, Renata; Demarta, Antonella; Tonolla, Mauro; Peduzzi, Raffaele

doi:10.1007/s002270050679

Cited by 48 publications

(35 citation statements)

References 18 publications

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“…Roseobacters are often most abundant in bacterial communities associated with marine algae, including natural phytoplankton blooms and algal cultures (see, e.g., references 4, 42, 78, 90, and 125). Roseobacter sequences are also abundant in communities associated with polar sea ice (16,17), diseased corals (21,80), sponges (111,119), hypersaline microbial mats (54), cephalopods (cuttlefish and squid) (9,46), scallop larvae (93), sea grasses (120), and coastal biofilms (24,25) (Table 2).…”

Section: Abundance and Distribution In Marine Environmentsmentioning

confidence: 99%

“…Ashen and Goff (8) identified Roseobacter phylotypes in three gallbearing species of the marine red alga Prionitis. Clade members are also dominant components of bacterial assemblages associated with the reproductive accessory nidamental glands in the cephalopods Loligo pealei (squid) and Sepia officinialis (cuttlefish) (9,46). Roseobacters have developed close associations with Pfiesteria and Pfiesteria-like species, where they are found within the nutrient-rich phycosphere of, or polarly attached to, these dinoflagellates (4).…”

Section: Emerging Physiologiesmentioning

confidence: 99%

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Overview of the Marine Roseobacter Lineage

Buchan

González

Moran

2005

Appl Environ Microbiol

765

738

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3Despite the overwhelming bacterial diversity present in the world's oceans, the majority of recognized marine bacteria fall into as few as nine major clades (36), many of which have yet to be cultivated in the laboratory. Molecular-based approaches targeting 16S rRNA genes demonstrate that the Roseobacter clade is one of these major marine groups, typically comprising upwards of 20% of coastal and 15% of mixed-layer ocean bacterioplankton communities (see, e.g., references 36, 37, 42, 98, and 109). Roseobacters are well represented across diverse marine habitats, from coastal to open oceans and from sea ice to sea floor (see, e.g., references 16, 28, 37, 42, 52, and 98). Members have been found to be free living, particle associated, or in commensal relationships with marine phytoplankton, invertebrates, and vertebrates (see, e.g., references 4, 6, 7, 44, 49, 115, and 119). Furthermore, representatives of the clade stand out as representing one of the most readily cultivated of the major marine lineages (36). These isolated representatives are serving as the foundation for an improved understanding of marine bacterial ecology and physiology. DESCRIPTION OF THE GROUPThe Roseobacter clade falls within the ␣-3 subclass of the class Proteobacteria, with members sharing Ͼ89% identity of the 16S rRNA gene. The first strain descriptions appeared in 1991, about the time that 16S rRNA-based approaches for cataloging microbial diversity were revealing the immensity of prokaryotic diversity in the world's oceans. Interest in the clade has risen steadily since the initial discovery of these strains; at present the clade contains 36 described species, representing 17 genera, and literally hundreds of uncharacterized isolates and clone sequences. The first described members were Roseobacter litoralis and Roseobacter denitrificans, both pinkpigmented bacteriochlorophyll a-producing strains isolated from marine algae (99). Subsequent cultivation of clade members, however, revealed that many strains are neither pink nor bacteriochlorophyll a producers (see, e.g., references 20, 41, 43, and 61). With the exceptions of the described strains of the genus Ketogulonicigenium (113) and several clones from a South African gold mine (GenBank accession numbers AF546906, -13, -17, -22 to -24, and -26), the Roseobacter clade is exclusively marine or hypersaline, with characterized isolates demonstrating either a salt requirement or tolerance (see, e.g., references 60 and 62 ABUNDANCE AND DISTRIBUTION IN MARINE ENVIRONMENTSBased on culture collections, 16S rRNA clone libraries, and single-cell analyses, roseobacters have been identified in most marine environments sampled. The group is prevalent in 16S rRNA gene inventories of seawater (Table 1) and marine sediments (Table 2) and is noticeably absent from analogous inventories of freshwater and terrestrial soil environments. Fluorescent in situ hybridization (FISH) studies quantifying Roseobacter populations in coastal waters of the southeastern United States and the North Sea indicate ...

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Section: Abundance and Distribution In Marine Environmentsmentioning

confidence: 99%

Section: Emerging Physiologiesmentioning

confidence: 99%

Overview of the Marine Roseobacter Lineage

Buchan

González

Moran

2005

Appl Environ Microbiol

765

738

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“…Phaeobacter were found in turbot larva rearings (Hjelm et al, 2004), cutaneous mucus of seahorses (Balcázar et al, 2010) and on cephalopods (Grigioni et al, 2000;Barbieri et al, 2001). In the Chinese Changjiang estuary, close relatives of P. gallaeciensis are among the most abundant bacterial groups (Sekiguchi et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Phaeobacter gallaeciensis genomes from globally opposite locations reveal high similarity of adaptation to surface life

et al. 2012

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Phaeobacter gallaeciensis, a member of the abundant marine Roseobacter clade, is known to be an effective colonizer of biotic and abiotic marine surfaces. Production of the antibiotic tropodithietic acid (TDA) makes P. gallaeciensis a strong antagonist of many bacteria, including fish and mollusc pathogens. In addition to TDA, several other secondary metabolites are produced, allowing the mutualistic bacterium to also act as an opportunistic pathogen. Here we provide the manually annotated genome sequences of the P. gallaeciensis strains DSM 17395 and 2.10, isolated at the Atlantic coast of north western Spain and near Sydney, Australia, respectively. Despite their isolation sites from the two different hemispheres, the genome comparison demonstrated a surprisingly high level of synteny (only 3% nucleotide dissimilarity and 88% and 93% shared genes). Minor differences in the genomes result from horizontal gene transfer and phage infection. Comparison of the P. gallaeciensis genomes with those of other roseobacters revealed unique genomic traits, including the production of iron-scavenging siderophores. Experiments supported the predicted capacity of both strains to grow on various algal osmolytes. Transposon mutagenesis was used to expand the current knowledge on the TDA biosynthesis pathway in strain DSM 17395. This first comparative genomic analysis of finished genomes of two closely related strains belonging to one species of the Roseobacter clade revealed features that provide competitive advantages and facilitate surface attachment and interaction with eukaryotic hosts.

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“…The accessory nidamental glands (ANGs) of female cephalopods are reproductive organs containing dense consortia of bacteria dominated by Alphaproteobacteria from the Roseobacter clade (5,11,13). Although the function of this organ has not been demonstrated, the production of antimicrobial and/or antifouling compounds from ANG bacterial isolates has been proposed (4,5).…”

mentioning

confidence: 99%

“…Besides being implicated in a number of largescale ecological roles, i.e., carbon cycling (12) and sulfur metabolism (10), roseobacters are also found as members of many eukaryotic-bacterial symbioses. For example, they form obligate associations with marine algae (1), are major colonizers of corals (2), and are commonly found as dominant members in the ANGs of several cephalopods-including loliginid squid (5), cuttlefish (11), and the Hawaiian bobtail squid Euprymna scolopes (unpublished data).…”

mentioning

confidence: 99%