Phylogenetic and phylogeographic relationships in Ixodes holocyclus and Ixodes cornuatus (Acari: Ixodidae) inferred from COX1 and ITS2 sequences

Song, Simon; Shao, Renfu; Atwell, R. B.; Barker, Stephen C.; Vankan, D.

doi:10.1016/j.ijpara.2011.03.008

Cited by 65 publications

(57 citation statements)

References 39 publications

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“…Sanger sequencing was performed in a 48 well plate with robotic instrumentation using T7 Transcription Start (5'-CTAATACGACTCACTATAGGG-3') universal sequencing primer (Promega, Madison, WI-USA). Samples were sequenced using an Applied Biosystems TM ABI 3730 DNA Analyzer and the conditions described by Song et al (2011). The sequencing reactions were done using the Big Dye  Terminator v3.1 cycle sequencing kit.…”

Section: Genetic Analysismentioning

confidence: 99%

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New insights from molecular characterization of the tick Rhipicephalus (Boophilus) microplus in Brazil

Csordas

Garcia

Cunha

et al. 2016

Rev. Bras. Parasitol. Vet.

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Section: Genetic Analysismentioning

confidence: 99%

“…The internal transcribed spacer 2 (ITS-2) is located between the 5.8S and 28S ribosomal subunits of rDNA (CRUICKSHANK, 2002). These genes are arranged in repeated units known as ribosomal cistrons, which have repeated copies and have been used to study phylogenetic relationships (CAMPBELL et al, 1993;SONG et al, 2011;BURGER et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

New insights from molecular characterization of the tick Rhipicephalus (Boophilus) microplus in Brazil

Csordas

Garcia

Cunha

et al. 2016

Rev. Bras. Parasitol. Vet.

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“…These include the second internal transcribed spacer region (ITS2) of the nuclear ribosomal gene cluster. Since the ITS2 region does not encode for proteins it is thought that it is under minimal selection pressure, which in turn allows for the accumulation of nucleotide substitutions (Barker, 1998;Coleman, 2003;Song et al, 2011). Thus, the genetic variation in this region may allow for the elucidation of evolutionary relationships within and between species.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity, acaricide resistance status and evolutionary potential of a Rhipicephalus microplus population from a disease-controlled cattle farming area in South Africa

Robbertse

Baron

Merwe

et al. 2016

Ticks and Tick-borne Diseases

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The Southern cattle tick, Rhipicephalus microplus is a hematophagous ectoparasite of great veterinary and economic importance. Along with its adaptability, reproductive success and vectoring capacity, R. microplus has been reported to develop resistance to the major chemical classes of acaricides currently in use. In South Africa, the Mnisi community in the Mpumalanga region offers a unique opportunity to study the adaptive potential of R.microplus. The aims of this study therefore included characterising acaricide resistance and determining the level and pattern of genetic diversity for R. microplus in this region from one primary population consisting of 12 communal dip-stations. The level of acaricide resistance was evaluated using single nucleotide polymorphisms (SNPs) in genes that contribute to acaricide insensitivity. Additionally, the ribosomal internal transcribed spacer 2 (ITS2) gene fragments of collected individuals were sequenced and a haplotype network was constructed. A high prevalence of alleles attributed to resistance against formamidines (amitraz) in the octopamine/tyramine (OCT/Tyr) receptor (frequency of 0.55) and pyrethroids in the carboxylesterase (frequency of 0.81) genes were observed. Overall, the sampled tick population was homozygous resistant to pyrethroid-based acaricides in the voltage-gated sodium channel (VGS) gene. A total of 11 haplotypes were identified in the Mnisi R.microplus population from ITS2 analysis with no clear population structure. From these allele frequencies it appears that formamidine resistance in the Mnisi community is on the rise, as the R. microplus populations is acquiring or generating these resistance alleles. Apart from rearing multi-resistant ticks to commonly used acaricides in this community these ticks may pose future problems to its surrounding areas.

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“…The traditional morphology based identification is sometimes problematic due to variations caused by blood meal [17] and chances of geographical strains of tick species having different vectorial capacity [18][19][20], genetic introgression, fertile hybrids [21][22], and resistance to acaricides [23] are always there. During the past two decades several molecular markers have been used to resolve relationships and solve problems facing systematics of hard ticks in the family Ixodidae [24][25][26][27][28][29][30][31][32][33][34][35][36][37][38][39][40]. In this context, there is lack of any study on molecular analysis of hard ticks from the India [2,41].…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic analysis of some hard ticks from India using mitochondrial 16s rDNA

2016

J App Biol Biotech

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The present study was conducted to analyze inter-relationships between Indian hard tick using mitochondrial 16s rDNA. The sequence alignment consisted of 85 sequences, 32 sequences of 16s rDNA belonging to 7 species of two genera viz. Rhipicephalus and Hyalomma generated from PCR amplified products and 53 sequences of hard ticks from India available in genbank database. The NJ analyses conducted in MEGA6 revealed that Haemaphysalinae is basal to the clade of Rhipicephalinae + Hyalomminae in the metastriate lineage while Ixodinae was basal in Ixodidae. There were two large clades, one clade of Hyalomma anatolicum, Hy. excavatum, Hy. hussaini and Hy. brevipunctata and second clade of Rhipicephalus (Rhipicephalus) + Rhipicephalus (Boophilus). The results provide evidence for the contention of polyphyly of Rh. (Rh.) sanguineus and species complex status of Rh. (B.) microplus. A further molecular study from wider distributional area using more genetic markers is needed to confirm these observations.

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Phylogenetic and phylogeographic relationships in Ixodes holocyclus and Ixodes cornuatus (Acari: Ixodidae) inferred from COX1 and ITS2 sequences

Cited by 65 publications

References 39 publications

New insights from molecular characterization of the tick Rhipicephalus (Boophilus) microplus in Brazil

New insights from molecular characterization of the tick Rhipicephalus (Boophilus) microplus in Brazil

Genetic diversity, acaricide resistance status and evolutionary potential of a Rhipicephalus microplus population from a disease-controlled cattle farming area in South Africa

Phylogenetic analysis of some hard ticks from India using mitochondrial 16s rDNA

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