Phylogenetic and genomic relationships in the genus Glycine Willd. based on sequences from the ITS region of nuclear rDNA

Abstract

Phylogenetic relationships among all 18 species of the genus Glycine were inferred from nucleotide sequence variation in the internal transcribed spacer (ITS) region of nuclear ribosomal DNA. Pairwise sequence divergence values ranged from 0.2% (a single nucleotide) between Glycine max and Glycine soja to 8.6% between Glycine hirticaulis and Glycine falcata. The length of the ITS1 and ITS2 sequences ranged from 215 to 238 nucleotides and from 205 to 222 nucleotides, respectively, and that of 5.8S was 168 nucleotides across all the species. Phylogenetic analyses of the ITS region clearly resolved all the genomic groups that were established previously based on cytogenetic and biochemical studies. Based on this study, we assign new genome symbols: HH to Glycine arenaria, H1H1 to Glycine hirticaulis, H2H2 to Glycine pindanica, II to Glycine albicans, and I1I1 to Glycine lactovirens. Parsimony analysis of the entire ITS region, using subgenus Soja as outgroup, resulted in a trichotomy consisting of the clades: G. falcata (F genome), Glycine cyrtoloba and Glycine curvata (C genome), and all other species (A, B, D, E, H, and I genomes) of the subgenus Glycine.

Patterns of Reproductive Isolation in Three Angiosperm Genera

“…Comparisons between allopatric and sympatric isolation were made using a randomization test in which the observed difference between slopes was compared to a distribution of differences generated from 1000 pseudorandom datasets; N, sample size; N spp , number of unique taxa used in crosses; P, value determined from randomization test (number of values exceeding the observed difference in slopes/1000 Our analyses in Glycine also highlight the influence that individual species with unusual crossing behavior can exert on overall patterns of reproductive isolation within a genus, and the utility of these analyses in identifying such taxa. Glycine falcata has been consistently recognized as an unusual member of its genus, on the basis of numerous autapomorphic morphological, mating, and biochemical features (Hermann 1962;Mies and Hymowitz 1973;Hymowitz and Singh 1987;Doyle et al 1990;Singh et al 1992;Kollipara et al 1997). For example, G. falcata is a primarily cleistogamous species with high selfing rates that are exaggerated by the presence of subterranean inflorescences-a unique morphological feature among the Glycine species we analyzed (Doyle et al 1990;Singh et al 1992).…”

Morfología y genética del árbol Anadenanthera colubrina var. cebil (Fabaceae) en Salta (noroeste de Argentina)

“…In fact, ITS based studies improved our knowledge on plant phylogeny providing direct evidence of species relationships, such as calcydenia or glycine (Baldwin, 1992(Baldwin, , 1993Baldwin et al, 1995;Kollipara, Singh, & Hymowitz, 1997). Also in other species such as fungi, as an example, high variability in ITS region allowed to better understand the infraspecific variability (Simon & Weiß, 2008;Schoch et al, 2012).…”

Ribosomal DNA variation in landraces of bambara groundnut

“…It is a conserved region that codes for ribosomal RNA (rRNA) which is involved in intra cellular protein synthesis (Kollipara et al, 1997). The ribosomal DNA consists of tandemly repeated units of 18S, 5.8S, 28S, intragenic spacer region (IGS) or non transcribed spacer (NTS) and internally transcribed spacer (ITS) as shown in Figure 1.…”

“…And it is this variation that is used to discriminate between individuals of the same or different species (Kollipara et al, 1997). For more than two decades now, the rDNA has been used as a powerful tool in comparative evolutionary and phylogenetic studies (Hillis and Davis, 1986;Mindell and Honeycutt, 1990;Wesson et al, 1992;Schlotterer et al, 1994;Vogler and DeSalle, 1994;Tang et al, 1996).…”