Phylogenetic and biochemical characterization of the oil-producing yeast Lipomyces starkeyi

Abstract

Lipomyces starkeyi is an oleaginous yeast, and has been classified in four distinct groups, i.e., sensu stricto and custers α, β, and γ. Recently, L. starkeyi clusters α, β, and γ were recognized independent species, Lipomyces mesembrius, Lipomyces doorenjongii, and Lipomyces kockii, respectively. In this study, we investigated phylogenetic relationships within L. starkeyi, including 18 Japanese wild strains, and its related species, based on internal transcribed spacer sequences and evaluated biochemical characters which reflected the phylogenetic tree. Phylogenetic analysis showed that most of Japanese wild strains formed one clade and this clade is more closely related to L. starkeyi s.s. clade including one Japanese wild strain than other clades. Only three Japanese wild strains were genetically distinct from L. starkeyi. Lipomyces mesembrius and L. doorenjongii shared one clade, while L. kockii was genetically distinct from the other three species. Strains in L. starkeyi s.s. clade converted six sugars, D-glucose, D-xylose, L-arabinose, D-galactose, D-mannose, and D-cellobiose to produce high total lipid yields. The Japanese wild strains in subclades B, C, and D converted D-glucose, D-galactose, and D-mannose to produce high total lipid yields. Lipomyces mesembrius was divided into two subclades. Lipomyces mesembrius CBS 7737 converted D-xylose, L-arabinose, D-galactose, and D-cellobiose, while the other L. mesembrius strains did not. Lipomyces doorenjongii converted all the sugars except D-cellobiose. In comparison to L. starkeyi, L. mesembrius, and L. doorenjongii, L. kockii produced higher total lipid yields from D-glucose, D-galactose, and D-mannose. The type of sugar converted depended on the subclade classification elucidated in this study.

Microbial lipid production from lignocellulosic hydrolyzates: effect of carbohydrate mixtures and acid‐hydrolysis byproducts on cell growth and lipid production by Lipomyces starkeyi

“…suggest that the yeast L. starkeyi can grow equally well on the individual sugars as on the mixtures of sugars. Similar results were reported by Zhao et al ., Oguri et al ., and Gong et al . The results presented in Fig.…”

“…Lipomyces starkeyi has the ability to grow on various carbon sources such as D‐glucose, L‐arabinose, D‐xylose, L‐galactose, D‐mannose and D‐cellobiose . There are recent reports dealing with cell growth and lipid production by L. starkeyi in the presence of byproducts of lignocellulosic hydrolyzates in the medium.…”

“…16 Lipomyces starkeyi has the ability to grow on various carbon sources such as D-glucose, L-arabinose, D-xylose, L-galactose, D-mannose and D-cellobiose. 17,18 There are recent reports dealing with cell growth and lipid production by L. starkeyi in the presence of byproducts of lignocellulosic hydrolyzates in the medium. Chen et al 19 reported that furfural at 0.5 g L −1 reduced the cell growth and lipid production significantly while 1 g L −1 of HMF reduced cell growth by 10% but there was no impact on lipid content.…”

Identification and Phylogenetic Analysis of Two Marine Petroleum-Degrading Yeasts from South China Sea Offshore Based on Magnetic Nanoparticles Extraction

“…Multiple sequence alignments were performed using the ClustalW software and the neighbor-joining(N-J) phylogenetic tree was constructed using Mega5.1 software. 35 …”

Efficiency of synthesis of extracellular polysaccharides strains of Lipomyces yeast

“…Дрожжи рода Lipomyces встречаются в почвах южного и северного полушария Земли, кроме высокогорных районов и почв тундры, где почвообразовательные процессы находятся на ранних стадиях развития, однако богаты этими дрожжами почвы степной и лесной зон [5].…”