Phylogenetic and antigenic characterization of new fish nodavirus isolates from Europe and Asia

Skliris, Georgios; Krondiris, John V.; Sideris, Diamantis C.; Shinn, Andrew P.; Starkey, W.; Richards, R. H.

doi:10.1016/s0168-1702(01)00225-8

Cited by 100 publications

(94 citation statements)

References 15 publications

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“…To date, nodavirus has been isolated from approximately 50 fish species worldwide and several studies have established that this virus is globally distributed in the environment [15,19,20]. In addition, the number of possible host species is increasing with increasing monitoring efforts.…”

Section: Discussionmentioning

confidence: 99%

“…In the Mediterranean, Nodaviruses from the RGNNV, SJNNV groups as well as reassortants have been detected [15][16][17]. Phylogenetic analyses [18][19][20] have revealed that genetic lineages of the Nodaviruses show low host specificity and generally correspond to geographic location. This finding suggests that genotypes have generally emerged due to spill-over from reservoirs that include a broad range of wild marine fish, although some isolates revealed links to commercial movement [4].…”

Section: Introductionmentioning

confidence: 99%

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Nodaviruses in Wild Fish Population Collected Around Aquaculture Cage Sites from Coastal Areas of Tunisia

Cherif¹,

Amdouni²

2017

Fish Aquac J

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This report describes the viral epidemiology of wild fish adjacent to cage farms within the Tunisian coasts and is focused on viral nervous necrosis virus (VNNV). A total of 92 apparently healthy wild marine fish were collected near aquaculture facilities in five different coastal areas of Tunisia. The brains and eyes of fish were examined by quantitative real time reverse transcriptase-polymerase chain reaction (qRT-PCR) to detect the nodavirus coat protein gene of. A total of 57 out of 92 (61.9%) samples were positive for nodavirus by qRT-PCR. This finding indicates that carrier fish occur at a considerable level in populations of wild marine fish. Samples from 13 fish species were found to be positive to the virus genome: Sarpa salpa, Trachurs trachurus, Boobs boops, Sardinella aurita, Diplodus vulgaris, Diplodus puntazzo Liza aurata, Diplodue sargus, Sparus aurata, Sardina pilchardus, Spicara maena, Spondyliosoma cantharus, and Diplodus annularis. The partial sequences of the RNA2 coat protein gene of these strains were identical with RGNNV type previously identified within farmed sea bass and sea bream species in Tunisia, with a homology >97%. With respect to the proximity of the sampling sites to the coast and to rearing facilities, results analysis can suggest that these viruses may be indigenous to Tunisian coastal waters.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Nodaviruses in Wild Fish Population Collected Around Aquaculture Cage Sites from Coastal Areas of Tunisia

Cherif¹,

Amdouni²

2017

Fish Aquac J

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“…Betanodavirus is a distinct group from the insect nodaviruses (Alphanodaviruses) [90] in the 7th report of the International Committee on Taxonomy of Viruses [8,86]. The recognized members are: barfin flounder nervous necrosis virus (BFNNV), red-spotted grouper nervous necrosis virus (RGNNV), striped jack nervous necrosis virus (SJNNV) and tiger puffer nervous necrosis virus (TPNNV) and RGNNV is most widely distributed [64,90,91,107].…”

Section: Aetiologymentioning

confidence: 99%

“…The open reading frame (ORF) contains a 982-amino-acid with a calculated molecular mass of 110.74 kDa. On the other hand, the smaller segment, RNA2 (1433 nt) having a G?C content of 53.24 % contains a 338-amino-acid major ORF which encodes the coat protein with a calculated molecular mass of 37.059 kDa flanked by 26 nt 5 0 -NTR and a 390 nt 3 0 -NTR [19,75,107,118]. RNA2 forms a major ORF of capsid protein and consists of a highly conserved region and a variable region.…”

Section: Genome Organizationmentioning

confidence: 99%

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Betanodavirus of Marine and Freshwater Fish: Distribution, Genomic Organization, Diagnosis and Control Measures

et al. 2012

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Characterization of Virus-like Particles Assembled in a Recombinant Baculovirus System Expressing the Capsid Protein of a Fish Nodavirus

Lin

Tang

et al. 2001

Virology

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Betanodaviruses are causative agents of neurological disorders in several species of fish. We cloned and sequenced the RNA2 segment of two grouper viruses isolated from Epinephelus malabaricus (malabaricus grouper nervous necrosis virus, MGNNV) and Epinephelus lanceolatus (dragon grouper nervous necrosis virus, DGNNV). The sequences of the two RNAs were 99% identical and comparison with previously sequenced RNA2 segments of fish nodaviruses striped jack nervous necrosis virus, Atlantic halibut virus, sea bass encephalitis virus, and greasy grouper nervous necrosis virus (GGNNV) revealed that MGNNV and DGNNV were most closely related to GGNNV. No correlation of sequence with geographical habitat was detected. The MGNNV coat protein, the gene product of RNA2, was expressed in Sf21 cells with a recombinant baculovirus system and virus-like particles (VLPs) spontaneously formed. Two types of VLPs were observed: a slower sedimenting particle was RNase-sensitive and stain-permeable, while the faster sedimenting particle survived RNase treatment and was not stain-permeable. An image reconstruction of the latter, obtained with electron cryomicroscopy data, revealed a morphology consistent with T = 3 quasi-symmetry but with features significantly different from insect nodavirus structures at the same resolution. This assembly system allows the first biophysical comparisons of fish and insect nodavirus structure, assembly, and stability.

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Phylogenetic and antigenic characterization of new fish nodavirus isolates from Europe and Asia

Cited by 100 publications

References 15 publications

Nodaviruses in Wild Fish Population Collected Around Aquaculture Cage Sites from Coastal Areas of Tunisia

Nodaviruses in Wild Fish Population Collected Around Aquaculture Cage Sites from Coastal Areas of Tunisia

Betanodavirus of Marine and Freshwater Fish: Distribution, Genomic Organization, Diagnosis and Control Measures

Characterization of Virus-like Particles Assembled in a Recombinant Baculovirus System Expressing the Capsid Protein of a Fish Nodavirus

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