Phylogenetic analysis of partial L1 gene of 10 human papillomavirus types isolated most commonly from women with normal and abnormal cervical cytology in Kuwait

Al‐Awadhi, Rana; Chehadeh, Wassim; Al-Jassar, Waleed; Al-Harmi, Jehad; Al-Saleh, Eyad; Kapila, Kusum

doi:10.1007/s00705-013-1658-y

Cited by 4 publications

(4 citation statements)

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“…This long control region of the HPV genome has been shown to be very informative and therefore very useful in the study of HPV variants [7, 12–14], as such it was used in this study. A close look at the phylogenetic tree (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…These are, a) lineage A (also known as European-Asian, EAS lineage), consisting of at least the sub-lineages A1, A2, A3, (known previously as European, E lineage) and A4 (previously known as Asian, As lineage); b) Lineage B (known previously as African-1 (Afr-1) lineage), consisting of at least two sub-lineages B1 (Afr-1a) and B2 (Afr-1b); c) Lineage C (known previously as African-2 (Afr-2) lineage), previously consisting of the sub-lineages Afr-2a and Afr-2b, and d) lineage D (Asian-American/North American, AA/NA lineage), consisting of at least the sub-lineages, D1 (known previously as North-American (NA) lineage), D2 (known previously as Asian-American-1 (AA1) lineage), and D3 (Asian-American-2 (AA2) lineage). These groups of lineages have more than 95% sequence homology in the long control region (LCR) [5, 7, 12–14]. …”

Section: Introductionmentioning

confidence: 99%

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Unique LCR variations among lineages of HPV16, 18 and 45 isolates from women with normal cervical cytology in Ghana

Awua

Adanu

Wiredu

et al. 2017

Virol J

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BackgroundIn addition to being useful for classification, sequence variations of human Papillomavirus (HPV) genotypes have been implicated in differential oncogenic potential and a differential association with the different histological forms of invasive cervical cancer. These associations have also been indicated for HPV genotype lineages and sub-lineages. In order to better understand the potential implications of lineage variation in the occurrence of cervical cancers in Ghana, we studied the lineages of the three most prevalent HPV genotypes among women with normal cytology as baseline to further studies.MethodsOf previously collected self- and health personnel-collected cervical specimen, 54, which were positive for HPV16, 18 and 45, were selected and the long control region (LCR) of each HPV genotype was separately amplified by a nested PCR. DNA sequences of 41 isolates obtained with the forward and reverse primers by Sanger sequencing were analysed.ResultsNucleotide sequence variations of the HPV16 genotypes were observed at 30 positions within the LCR (7460 – 7840). Of these, 19 were the known variations for the lineages B and C (African lineages), while the other 11 positions had variations unique to the HPV16 isolates of this study. For the HPV18 isolates, the variations were at 35 positions, 22 of which were known variations of Africa lineages and the other 13 were unique variations observed for the isolates obtained in this study (at positions 7799 and 7813). HPV45 isolates had variations at 35 positions and 2 (positions 7114 and 97) were unique to the isolates of this study.ConclusionThis study provides the first data on the lineages of HPV 16, 18 and 45 isolates from Ghana. Although the study did not obtain full genome sequence data for a comprehensive comparison with known lineages, these genotypes were predominately of the Africa lineages and had some unique sequence variations at positions that suggest potential oncogenic implications. These data will be useful for comparison with lineages of these genotypes from women with cervical lesion and all the forms of invasive cervical cancers.Electronic supplementary materialThe online version of this article (doi:10.1186/s12985-017-0755-z) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Unique LCR variations among lineages of HPV16, 18 and 45 isolates from women with normal cervical cytology in Ghana

Awua

Adanu

Wiredu

et al. 2017

Virol J

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“… 6 It is also frequently found in populations of adult women in countries such as Kuwait, Germany, Philippines, United States and Brazil. 7 …”

Section: Discussionmentioning

confidence: 99%

Detection of human papillomavirus in dental biofilm and the uterine cervix of a pregnant adolescent

et al. 2015

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CONTEXT: Adolescence and pregnancy are considered to be risk factors for human papillomavirus (HPV) infection. The relationship between this infection in the uterine cervix and oral HPV infection is controversial. CASE REPORT: This report describes a case of a pregnant 16-year-old adolescent who presented HPV infection in the uterine cervix and the mouth. Smears were collected from the cervix and the tongue/ palate. Dental biofilm samples were also collected. The microarray technique was used to detect HPV. The HPV 56 subtype was observed in the cervical smear and HPV 6 in dental biofilm. CONCLUSION: In this pregnant adolescent, HPV infection was present in both the cervix and the mouth, but the HPV subtypes infecting these two areas were different. RESUMO CONTEXTO:A adolescência e a gestação são consideradas fatores de risco para a infecção pelo papilomavírus humano (HPV). A relação entre essa infecção no colo do útero e na boca num mesmo paciente é controversa. RELATO DE CASO: Descrever o caso de uma adolescente grávida de 16 anos que apresentou a infecção pelo HPV no colo do útero e na boca. Esfregaços foram realizados no colo do útero e em língua/palato. Amostras de biofilme dental também foram coletadas. Para detectar o HPV, foi utilizada a técnica do microarranjo. O HPV 56 foi o subtipo encontrado no esfregaço cervical e o tipo HPV 6 no biofilme dental. CONCLUSÕES: Observamos, nessa adolescente grávida, a presença do HPV na boca e no colo do útero, mas os subtipos virais que infectavam essas duas regiões eram distintos. IMSc. Postgraduate Student,

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“…The primer sequences were as follows: WUT forward 5′-GGTACTCCCCATTATGCAGCC-3′ and WUT-reverse 5′-GGTTGGAGGGGCTGCAA-3′. PCR amplification was carried out as described previously [12].…”

Section: Amplification Of the Wuv Vp2 Typing Region And Phylogenetic mentioning

confidence: 99%

Human Washington University Polyomavirus in Patients with Respiratory Tract Infection in Kuwait

Essa¹,

Chehadeh²,

Al‐Nakib³

2017

Med Princ Pract

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Objective: To determine Washington University (WU) polyomavirus strains circulating among hospitalized patients with respiratory tract infections (RTI) in Kuwait. Materials and Methods: Samples from 459 hospitalized children and adult RTI patients were screened for respiratory viruses by polymerase chain reaction from April 2013 to April 2016. The VP2 gene from WU virus (WUV)-positive samples was sequenced and subjected to phylogenetic analysis. Results: Of the 459 hospitalized RTI patients, 18 (3.9%) patients were positive for WUV infection. WUV infection was common among children aged ≤11 years (9 patients, 50%). Among the 18 WUV-infected hospitalized patients, viral co-infection was detected in 9 patients (50%). The most common viruses associated with mixed infection were respiratory syncytial virus and human rhinovirus (2 patients, 11.1% each). Of the 18 WUV-infected patients, 4 were sequenced and subjected to phylogenetic analysis. The circulating strains belong to type Ia and IIIb. Conclusion: This study enabled us to detect WUV among hospitalized RTI patients. Co-infection with other respiratory viruses was notable. Two circulating WUV genotypes (Ia and IIIb) were identified among hospitalized RTI patients in Kuwait.

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Phylogenetic analysis of partial L1 gene of 10 human papillomavirus types isolated most commonly from women with normal and abnormal cervical cytology in Kuwait

Cited by 4 publications

References 39 publications

Unique LCR variations among lineages of HPV16, 18 and 45 isolates from women with normal cervical cytology in Ghana

Unique LCR variations among lineages of HPV16, 18 and 45 isolates from women with normal cervical cytology in Ghana

Detection of human papillomavirus in dental biofilm and the uterine cervix of a pregnant adolescent

Human Washington University Polyomavirus in Patients with Respiratory Tract Infection in Kuwait

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