Phylogenetic Analysis of Partial Bacterial 16S rDNA Sequences of Tropical Grass Pasture Soil under Acacia tortilis subsp. raddiana in Senegal

Diallo, Moudjahidou Demba; Martens, Miet; Vloemans, Nele; Cousin, Sylvie; Vandekerckhove, Tom; Neyra, Marc; Lajudie, Philippe de; Willems, Anne; Gillis, Monique; Vyverman, Wim; Gucht, Katleen Van der

doi:10.1078/072320204322881862

Cited by 18 publications

(4 citation statements)

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“…As suggested in other studies [3], [4], [5], we hypothesize that the availability of water affects the structure of microbial communities, resulting in different patterns during the dry and rainy seasons in the semi-arid climate of the Brazilian biome called Caatinga.…”

Section: Introductionsupporting

confidence: 55%

Water Regime Influences Bulk Soil and Rhizosphere of Cereus jamacaru Bacterial Communities in the Brazilian Caatinga Biome

et al. 2013

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We used the T-RFLP technique combined with Ion Torrent (PGM) sequencing of 16S rRNA and multivariate analysis to study the structure of bulk soil and rhizosphere bacterial communities of a cactus, Cereus jamacaru, from the Brazilian Caatinga biome, which is unique to Brazil. The availability of water shapes the rhizosphere communities, resulting in different patterns during the rainy and dry seasons. Taxonomic approaches and statistical analysis revealed that the phylum Actinobacteria strongly correlated with the dry season, while samples from the rainy season exhibited a strong correlation with the phylum Proteobacteria for rhizosphere samples and with the phyla Bacteroidetes, Firmicutes, Lentisphaerae, and Tenericutes for bulk soil samples. The STAMP software also indicated that the phylum Bacteroidetes, as well as two classes in the Proteobacteria phylum (γ and δ), were the most significant ones during the rainy season. The average abundance of the phylum Actinobacteria and the genus Bacillus was significantly greater during the dry season. Some significant genera found during the dry season might reflect their tolerance to the extreme conditions found in the Caatinga biome. They may also indicate the ecological function that microorganisms play in providing plants with some degree of tolerance to water stress or in assisting in their development through mechanisms of growth promotion. Alterations in microbial communities can be due to the different abilities of native microorganisms to resist and adapt to environmental changes.

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Section: Introductionsupporting

confidence: 55%

Water Regime Influences Bulk Soil and Rhizosphere of Cereus jamacaru Bacterial Communities in the Brazilian Caatinga Biome

et al. 2013

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“…After centrifugation at 6000 xg for 5 min, the supernatant was used for PCR. Amplification of 16S rDNA was performed in a 10 µl final volume containing 1 µl of total DNA, 10 µM of primer F27 (5′-AGAGTTTATCMTGGCTCAG-3′) and R1492 (5′-GRTACCTTGTTACGACTT-3′) [25], 10 mM of each dNTP, 5 mM MgCl 2 and 0.05U of Taq DNA polymerase (Eppendorf, Hamburg, Germany). A negative control (PCR mixture without DNA template) was included in all PCR experiments.…”

Section: Methodsmentioning

confidence: 99%

Native Bacterial Endophytes Promote Host Growth in a Species-Specific Manner; Phytohormone Manipulations Do Not Result in Common Growth Responses

2008

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BackgroundAll plants in nature harbor a diverse community of endophytic bacteria which can positively affect host plant growth. Changes in plant growth frequently reflect alterations in phytohormone homoeostasis by plant-growth-promoting (PGP) rhizobacteria which can decrease ethylene (ET) levels enzymatically by 1-aminocyclopropane-1-carboxylate (ACC) deaminase or produce indole acetic acid (IAA). Whether these common PGP mechanisms work similarly for different plant species has not been rigorously tested.Methodology/ Principal FindingsWe isolated bacterial endophytes from field-grown Solanum nigrum; characterized PGP traits (ACC deaminase activity, IAA production, phosphate solubilization and seedling colonization); and determined their effects on their host, S. nigrum, as well as on another Solanaceous native plant, Nicotiana attenuata. In S. nigrum, a majority of isolates that promoted root growth were associated with ACC deaminase activity and IAA production. However, in N. attenuata, IAA but not ACC deaminase activity was associated with root growth. Inoculating N. attenuata and S. nigrum with known PGP bacteria from a culture collection (DSMZ) reinforced the conclusion that the PGP effects are not highly conserved.Conclusions/ SignificanceWe conclude that natural endophytic bacteria with PGP traits do not have general and predictable effects on the growth and fitness of all host plants, although the underlying mechanisms are conserved.

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“…Total genomic DNA of the six selected bacterial isolates mentioned above was extracted from 1-day-old cultures. PCR procedure was carried out as described by Long et al 24 using primers F27 (5′-AGAGTTTATCMTGGC-TCAG-3′) and R1492 (5′-GRTACCTTGTTACGACTT-3′) 25 . A negative control (PCR mixture without DNA template) was included in all PCR reactions.…”

Section: Identification Of Selected Endophytic Bacterial Isolatesmentioning

confidence: 99%

Biological Control of Potato Tuber Soft Rot using <i>N</i>-Acyl-L-Homoserine Lactone-Degrading Endophytic Bacteria

Hà¹,

Minh²,

Hoi³

et al. 2018

Current Science

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Pectobacterium carotovorum (Pc) causing potato tuber soft rot uses N-acyl-L-homoserine lactones (AHLs) to control the production of virulence factors via quorum sensing (QS). Some bacteria produce enzymes to inactivate the AHL signals of pathogenic bacteria via a phenomenon known as quorum quenching. One hundred bacterial isolates from potato tubers were screened for AHL-degrading activity using biosensor strain Chromobacterium violaceum CV026. Of these isolates, 20 were able to inactivate AHLs from the pathogenic bacteria in vitro. Of the 20 isolates, 6 attenuated tissue maceration of potato tubers by Pc. Suppression of tuber soft rot was observed even when these isolates were applied 24 h after the pathogen was introduced. Their colonization in tubers was approximately 10 3 -10 4 cfu/g tuber, 7 days after inoculation. These isolates were identified as Bacillus sp., Variovorax sp., Variovorax paradoxus and Agrobacterium tumefaciens. Four of these isolates showed putative AHL-lactonase activity and provided the most significant protection against Pc. Therefore, AHL-degrading endophytic bacteria can be utilized as a novel biocontrol agent of potato tuber soft rot in Vietnam.

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Phylogenetic Analysis of Partial Bacterial 16S rDNA Sequences of Tropical Grass Pasture Soil under Acacia tortilis subsp. raddiana in Senegal

Cited by 18 publications

References 0 publications

Water Regime Influences Bulk Soil and Rhizosphere of Cereus jamacaru Bacterial Communities in the Brazilian Caatinga Biome

Water Regime Influences Bulk Soil and Rhizosphere of Cereus jamacaru Bacterial Communities in the Brazilian Caatinga Biome

Native Bacterial Endophytes Promote Host Growth in a Species-Specific Manner; Phytohormone Manipulations Do Not Result in Common Growth Responses

Biological Control of Potato Tuber Soft Rot using <i>N</i>-Acyl-L-Homoserine Lactone-Degrading Endophytic Bacteria

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