Phylogenetic Analysis of Parasitic Trematodes of the Genus<i>Euclinostomum</i>Found in Trichopsis and Betta Fish

Senapin, Saengchan; Phiwsaiya, Kornsunee; Laosinchai, Parames; Kowasupat, Chanon; Ruenwongsa, Pintip; Panijpan, Bhinyo

doi:10.1645/13-285.1

Cited by 21 publications

(10 citation statements)

References 17 publications

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“…as the only other member of the family Clinostomatidae for which sequences are available (COI: KC894795–KC894797) from three osphronemid fish, Trichopsis vittata, T. schalleri and Betta imbellis , in Thailand (Senapin et al . 2014) (Supplementary Table S1). This species, and the strigeids Alaria mustelae (JF904529) and Diplostomum baeri (GQ292501) were used as outgroups for rooting the trees.…”

Section: Methodsmentioning

confidence: 99%

Species delimitation in trematodes using DNA sequences: Middle-American Clinostomum as a case study

et al. 2016

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The recent development of genetic methods allows the delineation of species boundaries, especially in organisms where morphological characters are not reliable to differentiate species. However, few empirical studies have used these tools to delineate species among parasitic metazoans. Here we investigate the species boundaries of Clinostomum, a cosmopolitan trematode genus with complex life cycle. We sequenced a mitochondrial [cytochrome c oxidase subunit I (COI)] gene for multiple individuals (adults and metacercariae) from Middle-America. Bayesian phylogenetic analysis of the COI uncovered five reciprocally monophyletic clades. COI sequences were then explored using the Automatic Barcode Gap Discovery to identify putative species; this species delimitation method recognized six species. A subsample was sequenced for a nuclear gene (ITS1, 5·8S, ITS2), and a concatenated phylogenetic analysis was performed through Bayesian inference. The species delimitation of Middle-American Clinostomum was finally validated using a multispecies coalescent analysis (species tree). In total, five putative species are recognized among our samples. Mapping the second intermediate hosts (fish) onto the species tree suggests that metacercariae of these five species exhibit some level of host specificity towards their fish intermediate host (at the family level), irrespective of geographical distribution.

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Section: Methodsmentioning

confidence: 99%

Species delimitation in trematodes using DNA sequences: Middle-American Clinostomum as a case study

et al. 2016

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“…) and from Euclinostomum (Clinostomidae) (data from Senapin et al . ) were included as outgroups. Bayesian inference was based on five unlinked partitions of the data in which nucleotide substitution models and rates were expected to differ: ITS sequence, gaps in the ITS alignment (coded using FastGap v1.2, Borchsenius ) and one data set for each codon position in CO1, with Diplostomum baeri (Diplostomidae) designated as out‐group.…”

Section: Methodsmentioning

confidence: 99%

A large‐scale molecular survey ofClinostomum(Digenea, Clinostomidae)

et al. 2014

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Members of the genus Clinostomum Leidy, 1856 are parasites that mature in birds, with occasional reports in humans. Because morphological characters for reliable discrimination of species are lacking, the number of species considered valid has varied by an order of magnitude. In this study, sequences from the DNA barcode region of cytochrome c oxidase I (CO1) and/or internal transcribed spacer (ITS) from specimens from Mexico, Bolivia, Peru, Brazil, Kenya, China and Thailand were analysed together with published sequences from Europe, Africa, Indonesia and North America. Although ITS and CO1 distances among specimens were strongly correlated, distance‐based analysis of each marker yielded different groups. Putative species indicated by CO1 distances were consistent with available morphological identifications, while those indicated by ITS conflicted with morphological identifications in three cases. There was little overlap in sequence variation within and between species, particularly for CO1. Although ITS and CO1 distances tended to increase in specimens that were further apart geographically, this did not impair distance‐based species delineation. Phylogenetic analysis suggests a deep division between clades of Clinostomum inhabiting the New World and Old World, which parallels the distribution of their principal definitive hosts, the Ardeidae.

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“…A combination of molecular and morphological approaches is a useful way to resolve situations like this, as has already been shown in other clinostomids, i.e. Clinostomum Leidy, 1856 (Caffara et al ., 2011; Sereno-Uribe et al ., 2013), Euclinostomum Travassos, 1928 (Senapin et al ., 2014; Caffara et al ., 2016), Odhneriotrema Travassos, 1928 (Woodyard et al ., 2017) and Ithyoclinostomum Witenberg, 1925 (Briosio-Aguilar et al ., 2018). However, few DNA sequences from Clinostomoides are currently available for comparison.…”

Section: Introductionmentioning

confidence: 99%

Molecular data show Clinostomoides Dollfus, 1950 is a junior synonym of Clinostomum Leidy, 1856, with redescription of metacercariae of Clinostomum brieni n. comb.

et al. 2019

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The genus Clinostomoides Dollfus, 1950 was erected to accommodate a single worm from Ardea goliath sampled in the Belgian Congo. The specimen was distinguished from other clinostomids by its large size and posterior genitalia. In the following years, metacercariae of Clinostomoides brieni, have been described in Clarias spp. in southern and western Africa. A few authors have referred to Clinostomum brieni, but all such usages appear to be lapsus calami, and the validity of Clinostomoides remains widely accepted. In this study our aim was: position C. brieni among the growing clinostomids molecular database, and redescribe the species with emphasis on characters that have emerged as important in recent work. We sequenced two nuclear (partial 18S and ITS) and one mitochondrial marker (partial cytochrome c oxidase I) and studied morphology in metacercariae from hosts and localities likely to harbour the type species (Clarias spp., Democratic Republic of the Congo, South Africa). Phylogenetic analysis shows C. brieni belongs within Clinostomum Leidy, 1856. We therefore transfer C. brieni to Clinostomum, amend the diagnosis for the genus Clinostomum and provide a critical analysis of other species in Clinostomoides, all of which we consider species inquirendae, as they rest on comparisons of different developmental stages.

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Phylogenetic Analysis of Parasitic Trematodes of the GenusEuclinostomumFound in Trichopsis and Betta Fish

Cited by 21 publications

References 17 publications

Species delimitation in trematodes using DNA sequences: Middle-American Clinostomum as a case study

Species delimitation in trematodes using DNA sequences: Middle-American Clinostomum as a case study

A large‐scale molecular survey ofClinostomum(Digenea, Clinostomidae)

Molecular data show Clinostomoides Dollfus, 1950 is a junior synonym of Clinostomum Leidy, 1856, with redescription of metacercariae of Clinostomum brieni n. comb.

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