Phylogenetic analysis of nucleoprotein gene of Rabies virus in Malaysia from 2015 to 2018

Leow, B L; Khoo, C. K.; Aini, S Syamsiah; Roslina, H; Hanim, Mohd Saeid Faizah

doi:10.47665/tb.38.2.043

Cited by 4 publications

(6 citation statements)

References 8 publications

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“…Rabies virus from Sarawak (Malaysia) is genetically very close to the virus from East Kalimantan (Indonesia). This evidence is also supported by previous research in Malaysia [ 8 ], which showed that the virus from Sarawak is in a group with the virus from Indonesia. Still, the virus from Peninsular Malaysia is in a group of viruses from Thailand, Vietnam, and others [ 8 ].…”

Section: Discussionsupporting

confidence: 88%

“…This evidence is also supported by previous research in Malaysia [ 8 ], which showed that the virus from Sarawak is in a group with the virus from Indonesia. Still, the virus from Peninsular Malaysia is in a group of viruses from Thailand, Vietnam, and others [ 8 ]. Thus, Indonesian RABV generally tends to be similar to the virus from Sarawak and China (ASIA-3 cluster).…”

Section: Discussionsupporting

confidence: 88%

“…The N gene is the most conserved, so it is possible to accurately distinguish virus strains by analyzing their genetic differences [ 16 ]. Therefore, the N gene is very suitable as a basis for a molecular epidemiological study of rabies and its origin, genetic diversity, genetic identification of viral lineages, sublineages, and variants and as an analysis of their geographic distribution [ 8 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

“…In Asia, several countries have endemic rabies, such as India, Bangladesh, Philippines, Thailand, Indonesia, Myanmar, China (mainland China), and Korea [5][6][7]. Several districts and countries, including Hong Kong, Taiwan, Japan, and Malaysia, have been declared free of rabies, but sporadically, there are still cases of rabies in both animals and humans [6,8]. Not all areas in Indonesia are endemic to rabies, but only some parts of the islands of Sumatra, Kalimantan, Sulawesi, and Flores [9].…”

Section: Introductionmentioning

confidence: 99%

“…In general, the RABV genome encodes five structural proteins, namely, nucleoprotein (N), phosphoprotein (P), matrix protein (M), glycoprotein (G), and RNA-dependent RNA polymerase (L), which are sequentially arranged as 3'-N-P-M-G-L-5' [8,[15][16][17]. Among these five genes, there are different degrees of sequence conservation in the order of nucleoprotein > matrix protein > glycoprotein > phosphoprotein [15].…”

Section: Introductionmentioning

confidence: 99%

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Molecular analysis and geographic distribution of the recent Indonesian rabies virus

Cahyanti,

Syukur,

Purwati

et al. 2023

Vet World

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Background and Aim: Some Indonesian islands, including Sumatra, Kalimantan, Sulawesi, Java, and East Nusa Tenggara, have endemic rabies. Rabies outbreaks in Bali began from 2008 to 2011 and continue to occur sporadically. This study aimed to study the molecular analysis and geographical distribution of Indonesian rabies virus (RABV) from 2016 to 2021 and compare to previous periods. Materials and Methods: Virus isolates from 2016 to 2021 were extracted from dog brains and sequenced at the nucleoprotein gene locus. They were compared with data sequences available in the GenBank database. Indonesian RABV from the previous three periods (before 1989, 1997–2003, and 2008–2010) was extracted from the GenBank database. The genetic diversity in this study was based on the N gene of Indonesian RABV. Results: Asian RABV, which is genetically close to the Indonesian virus, is a virus from China (ASIA-3 cluster) and from the Southeast Asia region, namely, virus isolates from Sarawak and Malaysia and some Cambodian isolates. Rabies virus, which was isolated from the Bali islands, was the new cluster first detected and published in Bali, Indonesia, in 2008, while RABV from West Sumatra Province, which was isolated from 2016 to 2021, was also considered a new cluster that is genetically distant from other clusters in Indonesia. Conclusion: The RABV in Indonesia is divided into five clusters. The isolates from West Sumatra Province from 2016 to 2021 were a new cluster genetically distant from other Indonesian viruses. Keywords: genetic diversity, geographical distribution, nucleoprotein gene, rabies.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Molecular analysis and geographic distribution of the recent Indonesian rabies virus

Cahyanti,

Syukur,

Purwati

et al. 2023

Vet World

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Large-scale phylogenetic analysis reveals genetic diversity and geographic distribution of rabies virus in South-East and South Asia

Zhang

Sun

Gong

et al. 2023

Infection, Genetics and Evolution

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Molecular characterization of rabies virus from wild and domestic animals in the Sultanate of Oman

Ali,

Al Mawly

et al. 2024

Zoonoses and Public Health

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AimsRabies virus (RV) is endemic in some Arabian countries. However, it is difficult to control RV without understanding the epidemiological evolution of endemic RV isolates. The current study aimed to characterize RV from domestic and wild animal clinical cases in Oman.Methods and ResultsTwelve brain samples from domestic (Five camels, three goats and one cattle) and wild animals (Two foxes and one honey badger) were investigated from different locations in Oman between 2017 and 2020. All samples were confirmed by RV nucleoprotein (N) gene‐specific primers. Seven out of the 12 amplified samples were successfully sequenced and subjected to sequence and phylogenetic analysis. The detected RVs shared an in‐between 96.8%–98.7% and 96.9%–99% nucleotide and amino acid identities, respectively. However, the wild animal RVs shared only 92.6%–93.9% and 95.9% nucleotide and amino acid identities with the domestic animal RVs, respectively. Negri bodies were detected histologically in six brain samples from camels (n = 3), goats (n = 1) and foxes (n = 2). The RVs from domestic animals shared 97%–98.7% and 98%–100% nucleotide and amino acid identities with the previously published fox RVs from Oman and Gulf countries. Phylogenetic analysis suggested that all RV sequences belong to a distinct clade confined to the previously reported clade V within the Middle Eastern Cluster.ConclusionsAs indicated by the analysis of RVs from different locations between 2017 and 2020, a genetic variant isolated to the Gulf region may exist within the Middle East clade. Moreover, it appears that new RV lineages are emerging rapidly within this region. Therefore, a comprehensive genomic and phylogenetic analysis of the circulating RV is important for the development of future prevention and control strategies.

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Phylogenetic analysis of nucleoprotein gene of Rabies virus in Malaysia from 2015 to 2018

Cited by 4 publications

References 8 publications

Molecular analysis and geographic distribution of the recent Indonesian rabies virus

Molecular analysis and geographic distribution of the recent Indonesian rabies virus

Large-scale phylogenetic analysis reveals genetic diversity and geographic distribution of rabies virus in South-East and South Asia

Molecular characterization of rabies virus from wild and domestic animals in the Sultanate of Oman

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