Phylogenetic analysis of Metarhizium spp. isolated from soil in Japan

Nishi, Oumi; Hasegawa, Keiichi; Iiyama, Kazuhiro; Yasunaga-Aoki, Chisa; Shimizu, Susumu

doi:10.1007/s13355-011-0045-y

Cited by 30 publications

(25 citation statements)

References 29 publications

(53 reference statements)

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“…This habitat preference was indicated in two previous studies (Nishi et al 2011, 2013) and was confirmed in the current study. A comparison of in vitro germination of conidia demonstrated that the two species showed a higher germination rate under cold conditions and a lower germination rate under hot conditions compared with the other six species including Mpi (Nishi et al 2013).…”

Section: Discussionsupporting

confidence: 92%

“…in Japan from a previous study of Nishi et al (2011) and additional soil isolates were analysed in terms of species composition and habitat preferences. The results of the current study revealed the following: (1) 302 Japanese isolates were classified into 26 genotypes by PCR–RFLP of 5′-EF1-α and rDNA IGS and were identified as eight species by DNA sequence analysis of 5′-EF1-α; (2) Metarhizium spp.…”

Section: Discussionmentioning

confidence: 99%

“…in Japan were identified as eight species (Nishi et al 2011). These species were significantly associated with in vitro germination characteristics of conidia under high and low temperatures (Nishi et al 2013).…”

Section: Introductionmentioning

confidence: 99%

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Species associations and distributions of soil entomopathogenic fungi Metarhizium spp. in Japan

et al. 2017

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Metarhizium Sorokīn (Hypocreales: Clavicipitaceae) is a genus of facultative parasites of insects found in soils from various environments and is used for pest management. Understanding the habitat selection of Metarhizium spp. is critical to improve the efficacy and persistence of these fungi as microbial insecticides. This study sought to determine the habitat preferences of Metarhizium spp. in Japan. We identified 302 isolates of Metarhizium spp. as eight species by a combination of PCR–RFLP and phylogenetic analysis of DNA sequences. M. pingshaense was the predominant species in Japan and was most frequently isolated from both forest and agricultural environments. On the other hand, M. brunneum and M. pemphigi were comparatively restricted to forest environments. A similar species association was detected in a small area that was intensively investigated, where 7 species including 14 genotypes were isolated from soil. The results of this study have revealed different habitat preferences among Metarhizium spp. in Japan.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

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Species associations and distributions of soil entomopathogenic fungi Metarhizium spp. in Japan

et al. 2017

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“…The strains Fkk38-1, Kkj2-1, Kmm6-1, Myz9-2, Oit8-3, Okn1-1, and Sag14-1 were isolated from soil using oatmealbased semi-selective agar medium (6-% fine oat flour, 1.25-% agar, 0.03-% chloramphenicol, 0.1-% cycloheximide) (Nishi et al 2011); these were also identified as M. majus on the basis of the 5′-EF1-α sequence in the present study as well as in the study conducted by Nishi et al (2011). Fifteen isolates from the seven species of Metarhizium (M. anisopliae, M. brunneum, M. guizhouense, M. lepidiotae, M. pemphigi, M. pingshaense, and M. robertsii) only used for virulence comparisons were also isolated from soil and identified as each species in the same manner by Nishi et al (2011).…”

Section: Fungal Isolatesmentioning

confidence: 99%

Phylogenetic status and pathogenicity of Metarhizium majus isolated from a fruit beetle larva in Japan

et al. 2015

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Some isolates of Metarhizium anisopliae sensu lato with large conidia (MALC), which was formerly known as M. anisopliae var. majus, from a scarabaeid host, such as isolates from the coconut rhinoceros beetle (Oryctes spp.), have a narrow host range and are particularly adapted to their original host. Despite recent taxonomic revisions of the genus Metarhizium on the basis of DNA sequences, variations in host preferences of MALC have not been linked to DNA sequence variations. This study focused on the phylogenetic status and pathogenicity of MALC isolated from the fruit beetle larva Protaetia orientalis submarumorea (Pos) in Japan to investigate the relationship between virulence and nucleotide sequence variation among MALC. On the basis of the results of the phylogenetic analyses of DNA sequences from five loci, we identified an isolate from Pos (Hn1) as M. majus, which comprised many isolates with large conidia from scarabaeid insects. In the virulence assay of genetically diverse Metarhizium isolates, only Hn1 and its most closely related isolates from soil showed pathogenicity to Pos; however, these Pos-pathogenic isolates showed weak virulence against silkworms. In the analysis of the intergenic spacer region of rDNA, the Pos-pathogenic isolates displayed unique sequence variation and were clearly distinguished from closely related lineages, including isolates from Oryctes sp., which formed a separate monophyletic group. These results indicate that the Pos-pathogenic isolates from Japan are particularly adapted to Pos and can be genetically distinguished from M. majus isolates from different scarabaeid insects.

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“…The entomopathogenic fungus M. anisopliae is widely used for biocontrol of pest insects, and many commercial products are on the market or under development (Zimmermann, 2007;Nishi et al, 2011). There have been many attempts to use this fungus as a practical biocontrol agent against many insect pests, from termites in USA (Copping, 2001) to grasshoppers and locusts in Africa (Lomer et al, 2001).…”

Section: Disscussionmentioning

confidence: 99%

Virulence of entomopathogenic fungi against Plagiodera versicolora (Laicharting, 1781) (Coleoptera: Chrysomelidae)

Demir¹,

Seda²,

Emine³

et al. 2013

Afr. J. Agric. Res.

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The willow leaf beetle, Plagiodera versicolora (Laicharting, 1781), is one of the most destructive pests of many Salix and Populus species worldwide. A total of 12 entomopathogenic fungi including Beauveria bassiana (Bals.) Vuill., B. cf. bassiana, Metarhizium anisopliae (Metch) Sorok, and Isaria fumosorosea (Wize) (formerly Paecilomyces fumosoroseus) were tested against the larvae of this pest under laboratory conditions in order to find any possible fungal biocontrol agent against it. A conidial concentration of 1 × 10 7 conidia ml -1 for each isolate was applied to third instar larvae of the pest. Among tested fungal isolates, the highest mortality was obtained as 100% from B. bassiana KTU-57 within 14 days after inoculation (p < 0.05). This isolate also caused the highest mycosis value (83%) (p < 0.05). It was selected for dose-response test according to the screening test, and lethal concentration at 50% (LC 50 ) value of the fungus on the larvae of the pest was calculated as 1.03 × 10 5 conidia ml -1 based on probit analysis. Consequently, B. bassiana KTU-57 appears to be a significant promising isolate against the willow leaf beetle as a possible biocontrol agent.

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Phylogenetic analysis of Metarhizium spp. isolated from soil in Japan

Cited by 30 publications

References 29 publications

Species associations and distributions of soil entomopathogenic fungi Metarhizium spp. in Japan

Species associations and distributions of soil entomopathogenic fungi Metarhizium spp. in Japan

Phylogenetic status and pathogenicity of Metarhizium majus isolated from a fruit beetle larva in Japan

Virulence of entomopathogenic fungi against Plagiodera versicolora (Laicharting, 1781) (Coleoptera: Chrysomelidae)

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