Phylogenetic analysis of Metabacterium polyspora: clues to the evolutionary origin of daughter cell production in Epulopiscium species, the largest bacteria

Angert, Esther R.; Brooks, Austin E.; Pace, Norman R.

doi:10.1128/jb.178.5.1451-1456.1996

Cited by 45 publications

(30 citation statements)

References 35 publications

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“…polyspora' and its nearest relative, the polysporogenic anaerobe Clostridium lentocellum (Angert et al, 1996;Pace, 1996). The data obtained show that A .…”

Section: Status Of Anaerobacter Polyendosporusmentioning

confidence: 55%

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Note: Phylogenetic status of Anaerobacter polyendosporus, an anaerobic, polysporogenic bacterium

Сиунов

Nikitin²,

Сузина³

et al. 1999

International Journal of Systematic and Evolutionary Microbiology

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The almost complete sequence of the 16s rRNA gene of the Gram-positive polysporogenic bacterium Anaerobacter polyendosporus was determined. This allowed phylogenetic analysis of A. polyendosporus by comparing sequences Sciences, Pushchjno, Moscow region, 142292, Russia of the 165 rRNA gene of this bacterium to similar genes of other Gram-positive bacteria. It was shown that this polysporogenic bacterium belongs to the CIostridium cluster I, subcluster A. Phylogenetically, A. polyendosporus is distantly related to another polysporogenic, but non-cultivatable, bacterium, 'Metabacterium polyspora' and can be satisfactorily clustered within the saccharolytic clostridia with a low DNA G+C content grouped in subcluster A.A. polyendosporus was most closely related to Clostridium intestinale (94.8 Y o identity of 16s rRNA genes) and Clostridium fallax (93.1 %). Like other members of the CIostridium cluster I, subcluster A, A. polyendosporus possesses such common phenotypic features as a Gram-positive cell wall structure, anaerobiosis, derivation of energy from carbohydrate fermentation yielding butyric acid among other organic acids and the capacity for endogenous spore-formation. However, the scale of evolutionary change in the 16s rRNA gene between A. polyendosporus and phylogenetically related CIostridium species does not correspond to the profound changes in the phenotype of A. polyendosporus. Distinctive phenotypic features of the latter are large cell size, polysporogenesis (up to seven spores per cell), alternative modes of development and an unusual membrane ultrastructure.

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“…polyspora' and its nearest relative, the polysporogenic anaerobe Clostridium lentocellum (Angert et al, 1996;Pace, 1996). The data obtained show that A .…”

Section: Status Of Anaerobacter Polyendosporusmentioning

confidence: 55%

“…Data have recently been obtained on the almost complete sequence of the ' Metabacterium polyspora ' 16s rRNA gene, making it possible to define its phylogenetic relationship to other micro-organisms (Angert et al, 1996;Pace, 1996). However, the phylogenetic position of Anaerobacter is still obscure.…”

mentioning

confidence: 99%

Note: Phylogenetic status of Anaerobacter polyendosporus, an anaerobic, polysporogenic bacterium

Сиунов

Nikitin²,

Сузина³

et al. 1999

International Journal of Systematic and Evolutionary Microbiology

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“…These three tests have all been used to identify endospores in a variety of endospore-forming species. Specifically, the popping test has been used to distinguish endospores from vegetative cells in B. subtilis, Bacillus megaterium, and Metabacterium polyspora (3,40). For the popping test, samples were incubated in 200 l of 1 M HCl for 10 min at 60°C.…”

Section: Sample Collectionmentioning

confidence: 99%

“…The close phylogenetic relationship between M. polyspora and Epulopiscium spp. and morphological similarities between intracellular offspring production in these lineages suggest that the two reproductive processes are related (1,3).…”

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confidence: 99%

Nocturnal Production of Endospores in Natural Populations ofEpulopiscium-Like Surgeonfish Symbionts

et al. 2005

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Prior studies have described a morphologically diverse group of intestinal microorganisms associated with surgeonfish. Despite their diversity of form, 16S rRNA gene surveys and fluorescent in situ hybridizations indicate that these bacteria are low-G؉C gram-positive bacteria related to Epulopiscium spp. Many of these bacteria exhibit an unusual mode of reproduction, developing multiple offspring intracellularly. Previous reports have suggested that some Epulopiscium-like symbionts produce dormant or phase-bright intracellular offspring. Close relatives of Epulopiscium, such as Metabacterium polyspora and Clostridium lentocellum, are endospore-forming bacteria, which raises the possibility that the phase-bright offspring are endospores. Structural evidence and the presence of dipicolinic acid demonstrate that phase-bright offspring of Epulopiscium-like bacteria are true endospores. In addition, endospores are formed as part of the normal daily life cycle of these bacteria. In the populations studied, mature endospores were seen only at night and the majority of cells in a given population produced one or two endospores per mother cell. Phylogenetic analyses confirmed the close relationship between the endospore-forming surgeonfish symbionts characterized here and previously described Epulopiscium spp. The broad distribution of endospore formation among the Epulopiscium phylogenetic group raises the possibility that sporulation is a characteristic of the group. We speculate that spore formation in Epulopiscium-like symbionts may be important for dispersal and may also enhance survival in the changing conditions of the fish intestinal tract.

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“…appears to have evolved from bacterial endospore formation (3), based on the evolutionary relationship of Epulopiscium with endospore-forming bacteria (5,18) and morphologically defined stages that appear to be shared by the two processes (3)(4)(5). If intracellular offspring formation in Epulopiscium is related to endospore formation, we predict that genes involved early in sporulation will be conserved in Epulopiscium and used in offspring production (4).…”

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confidence: 99%

The spoIIE Homolog of Epulopiscium sp. Type B Is Expressed Early in Intracellular Offspring Development

et al. 2011

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Epulopiscium sp. type B is an enormous intestinal symbiont of the surgeonfish Naso tonganus. Intracellular offspring production in Epulopiscium shares features with endospore formation. Here, we characterize the spoIIE homolog in Epulopiscium. The timing of spoIIE gene expression and presence of interacting partners suggest that the activation of F occurs early in Epulopiscium offspring development.

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Phylogenetic analysis of Metabacterium polyspora: clues to the evolutionary origin of daughter cell production in Epulopiscium species, the largest bacteria

Cited by 45 publications

References 35 publications

Note: Phylogenetic status of Anaerobacter polyendosporus, an anaerobic, polysporogenic bacterium

Note: Phylogenetic status of Anaerobacter polyendosporus, an anaerobic, polysporogenic bacterium

Nocturnal Production of Endospores in Natural Populations ofEpulopiscium-Like Surgeonfish Symbionts

The spoIIE Homolog of Epulopiscium sp. Type B Is Expressed Early in Intracellular Offspring Development

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