Phylogenetic analysis of intestinal bacteria and their adhesive capability in relation to the intestinal mucus of carp

Namba, Aki; Mano, Nobuhiro; Hirose, Hajime

doi:10.1111/j.1365-2672.2006.03204.x

Cited by 53 publications

(37 citation statements)

References 30 publications

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“…The genus Aeromonas was significantly more abundant in the intestinal mucosa than in the intestinal content. These observations are consistent with previous studies reporting that Aeromonas with adhesive capability can colonise the surface of intestinal mucosa and that the intestine might be a primary location for stressinduced infections by Aeromonas [53]. However, bacteria associated with the mucosa may be regarded as indigenous species, and they are involved in host nutrition, mucosal defence and host immunity [54].…”

Section: −3supporting

confidence: 82%

Comparative Analysis of the Intestinal Bacterial Communities in Different Species of Carp by Pyrosequencing

et al. 2014

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Gut microbiota is increasingly regarded as an integral component of the host, due to important roles in the modulation of the immune system, the proliferation of the intestinal epithelium and the regulation of the dietary energy intake. Understanding the factors that influence the composition of these microbial communities is essential to health management, and the application to aquatic animals still requires basic investigation. In this study, we compared the bacterial communities harboured in the intestines and in the rearing water of grass carp (Ctenopharyngodon idellus), crucian carp (Carassius cuvieri), and bighead carp (Hypophthalmichthys nobilis), by using 454-pyrosequencing with barcoded primers targeting the V4 to V5 regions of the bacterial 16S rRNA gene. The specimens of the three species were cohabiting in the same pond. Between 6,218 and 10,220 effective sequences were read from each sample, resulting in a total of 110,398 sequences for 13 samples from gut microbiota and pond water. In general, the microbial communities of the three carps were dominated by Fusobacteria, Firmicutes, Proteobacteria and Bacteroidetes, but the abundance of each phylum was significantly different between species. At the genus level, the overwhelming group was Cetobacterium (97.29±0.46 %) in crucian carp, while its abundance averaged c. 40 and 60 % of the sequences read in the other two species. There was higher microbial diversity in the gut of filterfeeding bighead carp than the gut of the two other species, with grazing feeding habits. The composition of intestine microbiota of grass carp and crucian carp shared higher similarity when compared with bighead carp. The principal coordinates analysis (PCoA) with the weighted UniFrac distance and the heatmap analysis suggested that gut microbiota was not a simple reflection of the microbial community in the local habitat but resulted from species-specific selective pressures, possibly dependent on behavioural, immune and metabolic characteristics.

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Section: −3supporting

confidence: 82%

Comparative Analysis of the Intestinal Bacterial Communities in Different Species of Carp by Pyrosequencing

et al. 2014

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“…Pathogens as Escherichia coli that inhabit the gastrointestinal tract of pigs present surface proteins that recognise in a specific manner receptors in the mucus (Fang et al, 2000). Moreover these results support the previously observed by Namba et al (2007) who reported that intestinal bacteria which colonise the surface of intestinal mucosa, showed a high-adhesive capability to fish intestinal mucus. Adhesive strains isolated from faeces could be released to the lumen during the renewal of mucus.…”

Section: Resultssupporting

confidence: 82%

Potential probioticLactobacillus strains for piglets from an arid coast

Macías-Rodríguez

Zagorec²,

Ascencio

et al. 2008

Ann. Microbiol.

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Important centres for the pork industry have become growth in arid regions in the world and pig production needs alternatives to increase the productivity. A screening of predominant Lactobacillus strains from healthy piglets was performed in order to select specific probiotics. The ability of 164 strains to grow at different temperatures and concentrations of NaCl was evaluated. Results showed that all of them grew at 45 °C, 75% at 50 °C and 64% resisted 680 mM of salt. Adhesion to mucus and gastric mucin was evaluated showing 45% of strains isolated from faeces were able to adhere whereas 71% of strains from mucus showed mucus binding activity. Among the 164 isolates, 27 adhesive strains were identified using comparisons with 16S rDNA and intergenic 16-23S sequences. Results indicated that L. fermentum and L. reuteri were the most common species in faeces and mucus, respectively. Ability to grow in gastrointestinal mucus was evaluated showing that 92.6% of strains were able to replicate. Additionally, bacterial strain grown in 3.5% MRS with bile salts was evaluated. These results indicated that animals inhabiting isolated arid coasts are a rich source of probiotics, which resist adverse environmental conditions and can colonise the intestinal tract of pigs.

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“…The adhesive capability of intestinal bacteria is essential for colonization and growth in the intestinal tract (Namba et al, 2007). Hence, highly adhesive bacteria can reside for a longer period in intestinal tracts.…”

Section: Introductionmentioning

confidence: 99%

<i>Lactobacillus </i>Strain with High Adhesion Stimulates Intestinal Mucin Expression in Broiler

et al. 2012

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Lactobacillus fermentum 1.2029 strain with high adhesion was selected for studying the mucin expression of the small intestine epithelium of broilers after the strain was orally administrated. The strain was allowed to adhere to Caco-2 and to a hydrophobic solvent to evaluate its adhesive properties. Adhesion to intestinal mucin of chickens was also tested to further characterize the in vivo adhesion of the strain. Afterward, the acid and bile salt tolerance of the strain was also evaluated. L fermentum 1.2029 showed good adhesive ability and was able to survive in low pH conditions and in the presence of bile salts. The in vivo study showed that the strain significantly increased goblet cell density in the jejunum and the level of MUC2 mRNA in both the jejunum and ileum (P＜0.05). L. fermentum 1.2029 treatment didn't affect the thickness of the mucous layer. These results demonstrate that L. fermentum 1.2029 has protective functions, thereby regulating the goblet cell density and the expression of mucin in the small intestine of broilers. This work also suggests that L. fermentum 1.2029 is a potential probiotic feed additive for the improvement of the intestinal function of chickens.

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Phylogenetic analysis of intestinal bacteria and their adhesive capability in relation to the intestinal mucus of carp

Cited by 53 publications

References 30 publications

Comparative Analysis of the Intestinal Bacterial Communities in Different Species of Carp by Pyrosequencing

Comparative Analysis of the Intestinal Bacterial Communities in Different Species of Carp by Pyrosequencing

Potential probioticLactobacillus strains for piglets from an arid coast

<i>Lactobacillus </i>Strain with High Adhesion Stimulates Intestinal Mucin Expression in Broiler

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