Phylogenetic analysis of influenza A(H1N1)pdm viruses isolated in Ukraine during the 2009–2010 pandemic season

Abstract

To perform the phylogenetic analysis of segments, encoding hemagglutinin and neuraminidase of A(H1N1) pdm influenza viruses, isolated in Ukraine. Methods. In this study the real-time polymerase chain reaction (RT-PCR), sequencing and phylogenetic analysis methods were used. Results. Key mutations in amino acid sequences of proteins of Ukrainian pandemic influenza isolates were analyzed. High genetic similarity of Ukrainian and foreign pandemic isolates (99 %) was observed. Conclusions. The stability of Ukrainian isolates genes during 2009-2010 pandemic season was shown.