Phylogenetic analysis of classical swine fever virus (CSFV) field isolates from outbreaks in South and Central America

Pereda, Ariel; Greiser‐Wilke, I.; Schmitt, Beverly J.; Rincón, M. A.; Mogollón, J. D.; Sabogal, Z. Y.; Lora, Ángela; Sanguinetti, Héctor Ramón; Piccone, María E.

doi:10.1016/j.virusres.2005.01.011

Cited by 43 publications

(31 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The novel assays mentioned above are not suitable for subtyping the field isolates [5][6][7][8][9], except for the real-time RT-PCR which was able to discriminate CSFV at the major group level [7]. However, previous reports revealed that there was great diversity even within single subgroups which could be predominant in the field or even in one country [3,[10][11][12][13]. Therefore, subtyping at the major group level is not sufficient.…”

Section: Discussionmentioning

confidence: 98%

“…The disease has been eradicated in several countries or under control in other countries. Sporadical outbreaks were reported in the Americas and Asia as well as some European countries during the past few years [3,4,[10][11][12][13][16][17][18][19]. To control the disease in endemic areas, live attenuated C-strain vaccine has been proven to be efficacious and safe [20,21].…”

Section: Discussionmentioning

confidence: 99%

“…Recently, three multiplex real-time RT-PCR assays have been developed for differentiation [7][8][9], and one of the three methods could also differentiate CSFV isolates of three major groups [7]. However, isolates in a specific subgroup with great diversity were always predominant in the field or even in one country [3,[10][11][12][13]. Differentiation and subtyping of CSFV in a specific subgroup is not possible by the assays mentioned above.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Genetic characterization of E2 gene of classical swine fever virus by restriction fragment length polymorphism and phylogenetic analysis

Chen

Yuan

et al. 2010

Virus Genes

View full text Add to dashboard Cite

An RT-nested PCR (RT-nPCR)-based restriction fragment length polymorphism (RFLP) analyses of the E2 gene were developed for genetic subtyping and differentiation of vaccinated and infected classical swine fever virus (CSFV) strains. RT-nPCR identified 96 CSFV-positive samples from 321 clinical specimens from southeastern China during 2003-2008. The PCR products of positive samples were further differentiated using MspI digestion, 23 were identified as the C-strain, 62 as field strains, and 11 as mixture of the vaccine strain and field ones. RFLP with BglI, DdeI, DraI, and PstI were then used for subtyping of the field CSFV isolates. Thirty-eight field isolates phylogenetically classified as subgroup 2.1 based on E2 were divided into 11 subtypes by this RFLP scheme. Both RFLP profiling and sequence-based phylogenetic analysis revealed genetic diversity of CSFV in the field. Three novel substitutions at amino acid positions 17, 93, and 286 were identified in the predominant subtype VI strains isolated in 2008 as compared to other strains including historical subtype VI strains. These results suggest that CSFV in China experienced gradual variations and evolutionary accumulation progress. Thus, the RFLP methods targeting on the CSFV E2 gene are suitable for epidemiological survey in endemic area where the C-strain is applied for vaccination. Combination of the RFLP schemes with sequence-based phylogenetic analysis could provide more detailed information on transmission of CSFV in the region or even its evolution.

show abstract

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Genetic characterization of E2 gene of classical swine fever virus by restriction fragment length polymorphism and phylogenetic analysis

Chen

Yuan

et al. 2010

Virus Genes

View full text Add to dashboard Cite

show abstract

“…Phylogenetic analyses performed in different parts of the world confirmed that CSF virus isolates that differ by genetic typing seem to be characteristic for certain geographic regions (Bartak and Greiser-Wilke, 2000;Blacksell et al, 2005;Chen et al, 2008;Arce et al, 2005;Kamakawa et al, 2006;Li et al, 2006;Pereda et al, 2005;Sabogal et al, 2006;Stadejek et al, 1997;Vilcek et al, 1997).…”

Section: -140mentioning

confidence: 96%

Control and eradication of Classic Swine Fever in wild boar

2009

EFSA Journal

View full text Add to dashboard Cite

SummaryClassical swine fever (CSF) is a disease that has been causing major socio-economic damages in the EU during the last decades. Although considerable progress has been made in the eradication and prevention of the disease, the threat for an epidemic still exists.The virus is endemic in the wild boar population of several member states (MS). Wild boar cannot be managed as domestic pigs. Hunting and vaccination have been tentatively used in order to stop transmission by reducing the number of susceptibles. Oral vaccination of wild boar with modified live vaccine based on the C-strain (the only suitable) is used; this vaccine does not allow serological differentiation between vaccinated and infected animals.The aim of the control measures for CSF in wild boar is to reduce the risk of transmission to domestic pigs, to prevent an "endemic phase evolution" or to reduce the endemic phase duration.In order to support and to improve the control and eradication measures as regards CSF in wild boar, EFSA was requested by the Commission to provide scientific advice on the efficacy of the available surveillance, hunting and vaccination measures to control and eradicate CSF in feral pig populations (wild boar), considering the possible use of new diagnostic tests and vaccines.In order to reply to the mandate data were collected from MS through two questionnaires (CSF vaccines, hunting practices and CSF vaccination in wild boar). Data were also extracted from the EU CSF wild boar data base, after requesting permission from the participating MS.Considering that the data received were rather limited and not comparable, the WG decided to use a model in order to fill the gaps in the available knowledge. For the evaluation of the efficacy of CSF control in wild boar a continuous metapopulation compartmental model developed in an EU research project (FP6-5015599-CSFVACCINE&WILDBOAR) was applied.Although the disease manifests in the same manner in both domestic pig and wild boar it is more difficult to identify in wild boar because clinical monitoring of these is hardly possible. Wild boar found dead constitutes the main alert sign of CSF. Hunters and gamekeepers should be instructed to report the finding of each dead wild boar to the competent authority at all times. In case of high-risk situations, a passive surveillance should be complemented by an active serological surveillance. The active sampling of wild boar is not as efficient as in domestic pigs considering that hunting is the sole practical system to obtain samples and that the aim of hunting is quite different.Consequently, the sample size is not controlled by authorities and fits rarely the aim of the survey in terms of detecting the presence of disease at a certain level. In addition, the interpretation of serological results is confounded by maternal derived antibodies (until the 6th month of age), vaccination and the sampling quality.There is no definition at EU level of spatial and temporal units for surveillance, neither for criteria defining a disease free wi...

show abstract

“…For a fast diagnostic approach, short 59-NTR (150 nt)-and E2 (190 nt)-encoding sequence fragments are usually used for sequencing and subsequent typing. Based on these fragments, several studies have been published with CSFV isolates from all over the world (Bartak & Greiser-Wilke, 2000;Biagetti et al, 2001;Blacksell et al, 2004;Cha et al, 2007;Jemersic et al, 2003;Li et al, 2006;Pereda et al, 2005;Sabogal et al, 2006;Stadejek et al, 1997;Tu et al, 2001;Vilcek et al, 1997;Vlasova et al, 2003). Regarding these fragments, extensive datasets are available at the CRL .…”

Section: Discussionmentioning

confidence: 99%

Molecular epidemiology of current classical swine fever virus isolates of wild boar in Germany

Leifer

Hoffmann

Höper

et al. 2010

Journal of General Virology

View full text Add to dashboard Cite

Classical swine fever (CSF) has caused significant economic losses in industrialized pig production, and is still present in some European countries. Recent CSF outbreaks in Europe were mainly associated with strains of genogroup 2 (subgroup 2.3). Although there are extensive datasets regarding 2.3 strains, there is very little information available on longer fragments or whole classical swine fever virus (CSFV) genomes. Furthermore, there are no detailed analyses of the molecular epidemiology of CSFV wild boar isolates available. Nevertheless, complete genome sequences are supportive in phylogenetic analyses, especially in affected wild boar populations. Here, German CSFV strains of subgroup 2.3 were fully sequenced using two different approaches: (i) a universal panel of CSFV primers that were developed to amplify the complete genome in overlapping fragments for chain-terminator sequencing; and (ii) generation of a single full-length amplicon of the CSFV genome obtained by long-range RT-PCR for deep sequencing with next-generation sequencing technology. In total, five different strains of CSFV subgroup 2.3 were completely sequenced using these newly developed protocols. The approach was used to study virus spread and evolutionary history in German wild boar. For the first time, the results of our study clearly argue for the possibility of a long-term persistence of genotype 2.3 CSFV strains in affected regions at an almost undetectable level, even after long-term oral vaccination campaigns with intensive monitoring. Hence, regional persistence in wild boar populations has to be taken into account as an important factor in the continual outbreaks in affected areas.

show abstract

Phylogenetic analysis of classical swine fever virus (CSFV) field isolates from outbreaks in South and Central America

Cited by 43 publications

References 18 publications

Genetic characterization of E2 gene of classical swine fever virus by restriction fragment length polymorphism and phylogenetic analysis

Genetic characterization of E2 gene of classical swine fever virus by restriction fragment length polymorphism and phylogenetic analysis

Control and eradication of Classic Swine Fever in wild boar

Molecular epidemiology of current classical swine fever virus isolates of wild boar in Germany

Contact Info

Product

Resources

About