Phylogenetic Analysis of Canine Parvovirus VP2 Gene in Taiwan

Wang, Hsien‐Chi; Chen, Weida; Lin, Shiun-Long; Chan, Jacky Peng-Wen; Wong, Min‐Liang

doi:10.1007/s11262-005-1791-0

Cited by 48 publications

(45 citation statements)

References 10 publications

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“…A summary of our own experience of CPV detection during the last 8 years is presented in Table 6, which confirms that the sensitivity of VI (75.6%) is indeed lower than that of PCR (93.9%) although specificity is higher with VI (98.8%) than with PCR (94.1%). Therefore, as already described by ourselves and others [12,36], molecular methods such as PCR may be superior to others for routine detection of CPV and are very useful when known types of CPV are investigated in a large number of field samples [6,8,9,11,15,21,22,28,33,48,59]. In the present study, our routine PCR did reveal that the field CPV-2a or 2b viruses were decisively associated with all clinical cases ( Table 1).…”

Section: Discussionsupporting

confidence: 73%

“…This is a non-synonymous substitution that does not result in an antigenic change and thus new CPV-2a and 2b are genetic but not antigenic variants of the prototype CPV-2a and 2b. Today, the new CPV-2a and 2b have become the predominant CPV throughout the world, although the relative proportion of antigenic types varies from country to country [8,9,13,15,21,28,33,38,48,56,59]. Our recent study indicates that the new CPV-2b has been predominant since 1997 over the field of Japan [39].…”

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confidence: 87%

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Recombination Between Vaccine and Field Strains of Canine Parvovirus is Revealed by Isolation of Virus in Canine and Feline Cell Cultures

et al. 2008

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ABSTRACT. Canine parvovirus type 2 (CPV) is a pathogen that causes severe hemorrhagic gastroenteritis with a high fatality rate in pups worldwide. Since CPV emerged in the late 1970s, its origin has been explored with the conclusion that CPV originated from feline panleukopenia virus or a closely related virus. Both high mutation rate and recombination are assumed to be key factors in the evolution of parvoviruses. Here we provide evidence for natural recombination in CPV isolated from dogs in cell culture. Antigenic and genetic properties of isolates from 10 diseased pups were elucidated. Six pups had been vaccinated beforehand with live combined vaccine containing original antigenic type CPV (CPV-2). Six isolates recovered from 4 vaccinated pups in cell cultures were found to contain either CPV-2 or CPV-2-like viruses. The other isolates, including all those from non-vaccinated pups, were CPV-2b viruses. Antigenic typing of two CPV-2-like isolates, 03-029/M and 1887/f, with a monoclonal antibody panel suggested they were a mixture of CPV-2 and CPV2a (03-029/M) and a recombinant of CPV-2 and CPV-2b (1887/f). Genetic analysis of the VP1 gene indicated that isolate 03-029/M was a mixture of CPV-2, CPV-2a and a recombinant of CPV-2 and CPV-2a viruses, while isolate 1887/f was composed of a recombinant of CPV-2 and CPV-2b viruses. This is the first demonstration of natural CPV recombination in the field and suggests that recombination in the evolution of CPV is a more frequent and important process than previously believed. KEY WORDS: canine parvovirus, cell culture, dog, parvovirus, recombination.J. Vet. Med. Sci. 70(12): 1305-1314 Canine parvovirus type 2 (CPV), one of the feline parvovirus (FPV) subspecies, emerged suddenly throughout the world in the late 1970s as a new pathogen that caused severe hemorrhagic gastroenteritis and myocarditis in domestic dogs [1,2,7,20,23,40]. Mortality rate is usually high, particularly in non-immune pups. The origin of the virus is still not clear but the most likely hypothesis from phylogenetic analysis is that CPV originated from feline panleukopenia virus (FPLV) or a very closely related carnivore parvovirus of feral canids, such as foxes and mink [52,55]. There has been speculation that, during circulation for some time in an European local dog population, this ancestral CPV gradually adapted for domestic dogs and the emergent original CPV (antigenic type 2: CPV-2) rapidly spread globally as a new pathogen of domestic dogs.In the early 1980s, this first CPV-2 disappeared from the field having been replaced by a new antigenic variant designated CPV-2a, and another antigenic variant CPV-2b appeared soon afterwards. These variants can be distinguished by monoclonal antibodies (MAbs) [35,43,[45][46][47]. CPV-2a and 2b use both canine and feline transferrin receptors for binding to cells both in vitro and in vivo [17,42] and consequently can infect dogs as well as cats [34,56]. In contrast, CPV-2 can infect both feline and canine cells in vitro but infects only dogs in vivo [57]...

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Section: Discussionsupporting

confidence: 73%

mentioning

confidence: 87%

Recombination Between Vaccine and Field Strains of Canine Parvovirus is Revealed by Isolation of Virus in Canine and Feline Cell Cultures

et al. 2008

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“…2). The results were in agreement with those reported by Hirasawa et al [7] in Japan, Steniel et al [19] in South Africa, Pereira et al [14] in Brazil, Wang et al [24] in Taiwan and Hong et al [8] in US. In a recent report Sanjukta et al [17] also reported CPV-2b as the prevalent strain in certain North Indian states.…”

supporting

confidence: 93%

Molecular Typing of Canine parvovirus Occurring in Pondicherry by Multiplex PCR and PCR–RFLP

Parthiban¹,

Mukhopadhyay²,

Antony³

et al. 2010

Indian J. Virol.

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The present study was aimed at molecular typing of Canine parvovirus (CPV) occurring in Pondicherry using PCR based assays. CPV-2a and CPV-2b types were detected by PCR in 68 (53.12%) out of 128 faecal samples/rectal swabs. All the 68 samples found positive by PCR assay were subjected to multiplex PCR assay with CPV-2ab and CPV-2b primer pairs. Sixty-seven (98.52%) samples were characterized as CPV-2b type and one sample (1.47%) was categorized as CPV-2a type. Sixty clinical samples found negative by CPV-2ab primers were subjected to another PCR assay with CPV-555 primer pair for detecting CPV-2c. Though three samples (5%) responded to this PCR, subsequent RFLP of these PCR products with MboII did not show any cleavage indicating the absence of CPV-2c in Pondicherry. It was inferred that CPV-2b was the most prevalent CPV type in Pondicherry. It was further concluded that the CPV-2 variants (CPV-2a, CPV-2b and CPV-2c) currently circulating in the field worldwide could be diagnosed by employing multiplex PCR and PCR-RFLP assays.

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“…As it was expected and reported previously (Decaro et al 2009) all the CPV-2c VP2 sequences formed a monophyletic cluster (cluster 7). Despite the strong phylogenetic association with CPV-2a ancestor, the Lithuanian CPV VP2 sequences show more or less geographically defined evolution pattern (especially five CPV samples in the first cluster), as were identified in other regions and studies (Battilani et al 2002, Wang et al 2005, Kang et al 2008. Phylogenetic analysis showed some evidence for geographical clustering at an international level, suggesting that currently there are limited opportunities for global transmission, as has previously been suggested by others (Hoelzer et al 2008).…”

Section: Discussionmentioning

confidence: 49%

Phylogenetic characterization of Canine Parvovirus VP2 partial sequences from symptomatic dogs samples

Zienius¹,

Lelešius²,

Kavaliauskis³

et al. 2016

Polish Journal of Veterinary Sciences

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The aim of the present study was to detect canine parvovirus (CPV) from faecal samples of clinically ill domestic dogs by polymerase chain reaction (PCR) followed by VP2 gene partial sequencing and molecular characterization of circulating strains in Lithuania.Eleven clinically and antigen-tested positive dog faecal samples, collected during the period of 2014-2015, were investigated by using PCR. The phylogenetic investigations indicated that the Lithuanian CPV VP2 partial sequences (3025-3706 cds) were closely related and showed 99.0-99.9% identity. All Lithuanian sequences were associated with one phylogroup, but grouped in different clusters. Ten of investigated Lithuanian CPV VP2 sequences were closely associated with CPV 2a antigenic variant (99.4% nt identity). Five CPV VP2 sequences from Lithuania were related to CPV-2a, but were rather divergent (6.8 nt differences). Only one CPV VP2 sequence from Lithuania was associated (99.3% nt identity) with CPV-2b VP2 sequences from France, Italy, USA and Korea. The four of eleven investigated Lithuanian dogs with CPV infection symptoms were vaccinated with CPV-2 vaccine, but their VP2 sequences were phylogenetically distantly associated with CPV vaccine strains VP2 sequences (11.5-15.8 nt differences).Ten Lithuanian CPV VP2 sequences had monophyletic relations among the close geographically associated samples, but five of them were rather divergent (1.0% less sequence similarity). The one Lithuanian CPV VP2 sequence was closely related with CPV-2b antigenic variant. All the Lithuanian CPV VP2 partial sequences were conservative and phylogenetically low associated with most commonly used CPV vaccine strains.

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Phylogenetic Analysis of Canine Parvovirus VP2 Gene in Taiwan

Cited by 48 publications

References 10 publications

Recombination Between Vaccine and Field Strains of Canine Parvovirus is Revealed by Isolation of Virus in Canine and Feline Cell Cultures

Recombination Between Vaccine and Field Strains of Canine Parvovirus is Revealed by Isolation of Virus in Canine and Feline Cell Cultures

Molecular Typing of Canine parvovirus Occurring in Pondicherry by Multiplex PCR and PCR–RFLP

Phylogenetic characterization of Canine Parvovirus VP2 partial sequences from symptomatic dogs samples

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