Phylogenetic analysis of atmospheric halotolerant bacterial communities at high altitude in an Asian dust (KOSA) arrival region, Suzu City

Maki, Takeshi; Susuki, Shinzi; Kobayashi, Fuminori; Kakikawa, Makiko; Tobo, Yutaka; Yamada, Masatoshi; Higashi, T.; Matsuki, Atsushi; Hong, Chun-Sang; Hasegawa, Hiroshi; Iwasaka, Yasunobu

doi:10.1016/j.scitotenv.2010.04.002

Cited by 100 publications

(70 citation statements)

References 41 publications

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“…3 and 4a). Some clones recovered from the 3 air samples were identical to B. subtilis, which were detected at high altitudes above the Taklamakan Desert (Maki et al, 2008) and were associated with the Asian dust mineral particles collected in downwind areas (Maki et al, 2010;Jeon et al, 2011;Tanaka et al, 2011;Maki et al, 2014;Fig. 4a).…”

Section: Bacterial Populations Associated With Dust Eventsmentioning

confidence: 95%

“…Dust events are known to transport airborne microorganisms, thereby supporting the microbial immigration to downwind ecosystems (Griffin et al, 2007;Maki et al, 2010;Yamaguchi et al 2012). In Asian regions, airborne microorganisms transported over long distances increase allergenic burden, consequently increasing the incidence of asthma (Ichinose et al, 2005) and contributing to the dispersion of diseases such as Kawasaki disease in humans (Rodó et al, 2011) and rust diseases in plants (Brown and Hovmøller, 2002).…”

Section: Introductionmentioning

confidence: 99%

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Vertical distribution of airborne bacterial communities in an Asian-dust downwind area, Noto Peninsula

Maki

Hara

Kobayashi

et al. 2015

Atmospheric Environment

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Bioaerosols at high altitudes were collected using an aircraft and a balloon.Pyrosequencing using 16S rDNA revealed vertical distributions of airborne bacteria.16S rDNA clone libraries suggested the transport of terrestrial and marine bacteria.Meteorological conditions contribute to vertical variations in airborne bacteria. 3 AbstractBacterial populations transported from ground environments to the atmosphere get dispersed throughout downwind areas and can influence ecosystem dynamics, human health, and climate change. However, the vertical bacterial distribution in the free troposphere was rarely investigated in detail. We collected aerosols at altitudes of 3,000 m, 1,000 m, and 10 m over the Noto Peninsula, Japan, where the westerly winds carry aerosols from continental and marine areas. During the sampling period on March 10, 2012, the air mass at 3,000 m was transported from the Chinese desert region by the westerly winds, and a boundary layer was formed below 2,000 m. Pyrosequencing targeting 16S rRNA genes (16S rDNA) revealed that the bacterial community at 3,000 m was predominantly composed of terrestrial bacteria, such as Bacillus and Actinobacterium species. In contrast, those at 1,000 m and 10 m included marine bacteria belonging to the classes Cyanobacteria and Alphaproteobacteria. The entire 16S rDNA sequences in the clone libraries were identical to those of the terrestrial and marine bacterial species, which originated from the Chinese desert region and the Sea of Japan, respectively. The origins of air masses and meteorological conditions contribute to vertical variations in the bacterial communities in downwind atmosphere.

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Section: Bacterial Populations Associated With Dust Eventsmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Vertical distribution of airborne bacterial communities in an Asian-dust downwind area, Noto Peninsula

Maki

Hara

Kobayashi

et al. 2015

Atmospheric Environment

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“…We analyzed the effects of Asian dust in the 86 patients enrolled in our study. Further studies with a larger number of patients are needed to reveal the environmental factors, including pollens, chemicals, weather conditions and bioaerosols, which could adversely affect these respiratory diseases (Griffin, 2007;Maki et al, 2010;Tobo et al, 2010), and to demonstrate whether the symptoms could vary in terms of asthma, cough variant asthma and atopic cough.…”

Section: Discussionmentioning

confidence: 99%

Exacerbation of daily cough and allergic symptoms in adult patients with chronic cough by Asian dust: A hospital-based study in Kanazawa

Higashi

Kambayashi

Ohkura

et al. 2014

Atmospheric Environment

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AbbreviationsTotal suspended particulate (TSP); Particulate matter (PM); Light detection and ranging (LIDAR); Polycyclic aromatic hydrocarbons (PAHs) 1 AbstractThe health effects associated with Asian dust have attracted attention due to the rapid increase in the number of Asian dust events in East Asia in recent years. The aim of this study was to investigate the associations between Asian dust and daily cough, as well as allergic symptoms, in adult patients who suffer from chronic cough. We enrolled 86 adult patients from Kanazawa University Hospital, Japan, who were diagnosed with asthma, cough variant asthma, atopic cough or a combination of these conditions. From January to June 2011, subjects recorded their symptoms in a diary every day. Asian dust and non-Asian dust periods were defined according to the dust extinction coefficient, measured using the light detection and ranging (LIDAR). The daily levels of total suspended particulates, polycyclic aromatic hydrocarbons (PAHs) and coexisting factors related to allergies, such as the Japanese cedar pollen count, were measured.McNemar's test showed that there were significantly more cough-positive patients during Asian dust periods than during the non-Asian dust period (p = 0.022). In addition, during Asian dust periods when the daily levels of Japanese cedar pollen, Japanese cypress pollen and PAHs were elevated, there were significantly more patients who experienced itchy eyes than during the non-Asian dust period (p < 0.05). On the other hand, there were no significant differences in the allergic symptoms, including sneezing or a runny nose and nasal congestion. This is the first report to show that Asian dust triggers cough and allergic symptoms in adult patients with chronic cough.

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“…The members of B. subtilis group including halotolerant bacteria were dominantly associated with dust mineral particles collected at altitudes some hundreds of meters above the Taklamakan Desert (Maki et al 2008) and Suzu City during Kosa events (Maki et al 2010). In the snow cover of Mt.…”

Section: Discussionmentioning

confidence: 99%

“…In previous studies, aerosol sampling, using a balloon (Kobayashi et al 2007) and a tower (Li et al 2010) at altitudes ranging from 200m to 800m demonstrated that bioaerosols are composed of several species of bacteria. In particular, members of the genus Bacillus were associated with Kosa mineral particles collected at high altitudes in downwind area during Kosa events (Maki et al 2010). It has been reported that airborne microbial communities at ground level in Asian regions change significantly in species composition and abundance depending on Kosa events (Hara and Zhang 2012).…”

Section: Introductionmentioning

confidence: 98%

NaCl-amendment assay targeting airborne bacteria in tropospheric bioaerosols transported by westerly wind over Noto Peninsula

et al. 2012

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Bioaerosol particles including bacteria, fungi and virus are originated from marine and terrestrial environments. The airborne microorganisms are transported for long-distance through the free troposphere and are thought to influence the downwind ecosystems and human life. However, microbial communities in the free troposphere have not been understood in detail because the direct sampling of microbial cells at high altitude requires sophisticated sampling techniques. In this study, for the investigation of microbial species compositions in the free troposphere, air sampling using an aircraft was performed over the Noto Peninsula in Japan, where the tropospheric winds carry aerosol particles from continental areas. Two air samples were collected at 3000 m on March 27th, 2010, when air mass was carried from the Gobi Desert to Japan area.Microorganisms from one air sample grew in culture media containing up to 15% NaCl, suggesting that halotolerant bacteria maintain their viabilities in the free troposphere. DGGE analysis revealed that the amended cultures were dominated by Bacillus subtilis, and the isolates obtained from the amended cultures were identical to B. subtilis.Furthermore, the 16S rDNA clone library (culture independent survey) of the other air sample grew was composed of three phylotypes belonging to Firmicutes, Bacteroidetes, and Proteobacteria with the sequences of Firmicutes phylotype corresponding to that of the cultured B. subtilis sequence. Microscopic observation using FISH method indicated that B. subtilis particles occupied 80% of total eubacterial particles on the mineral particles. The halotolerant bacteria identical to B. subtilis would dominate at high altitudes over Noto Peninsula where the prevailing westerly wind was blowing.2

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Phylogenetic analysis of atmospheric halotolerant bacterial communities at high altitude in an Asian dust (KOSA) arrival region, Suzu City

Cited by 100 publications

References 41 publications

Vertical distribution of airborne bacterial communities in an Asian-dust downwind area, Noto Peninsula

Vertical distribution of airborne bacterial communities in an Asian-dust downwind area, Noto Peninsula

Exacerbation of daily cough and allergic symptoms in adult patients with chronic cough by Asian dust: A hospital-based study in Kanazawa

NaCl-amendment assay targeting airborne bacteria in tropospheric bioaerosols transported by westerly wind over Noto Peninsula

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