Phylogenetic Analysis of Astigmatid Mites Sarcoptes scabiei and Dermatophagoides farinae using ITS-2 as a Genetic Marker

Naz, Shumaila; Chaudhry, Farhana Riaz; Rizvi, Dilawar Abbas; Ismail, Muhammad

doi:10.37185/lns.1.1.75

Cited by 3 publications

(2 citation statements)

References 18 publications

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“…Portions of the cutaneous samples stored at −20°C were macerated in liquid nitrogen, and genomic DNA was extracted from each sample using a Universal Genomic DNA Extraction Kit (D2100; Solarbio, China) and subjected to PCR analysis. Fragments of the cytochrome oxidase 1 (cox‐1) (374 bp) and the second internal transcribed spacer gene (ITS2) (361 bp) genes of S. scabiei were amplified ( S2 ) (Fraser et al., 2018; Naz et al., 2020). The amplification products were sequenced by Sangon Biotech (Shanghai) Co., Ltd and identified by BLAST (NCBI).…”

Section: Methodsmentioning

confidence: 99%

Sarcoptic mange is an emerging threat to biodiversity in the Qinling Mountains in China

Chen

Zhang

et al. 2022

Transbounding Emerging Dis

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Sarcoptic mange, a disease caused by the burrowing mite Sarcoptes scabiei, is globally endemic and an emerging threat to wildlife. Although many studies have shown that wildlife diseases play key roles in biodiversity conservation, knowledge about sarcoptic mange is still insufficient. In this study, we aim to improve the understanding of the impacts of sarcoptic mange on wildlife populations, the mechanisms involved in its eco‐epidemiology and the associated risks to public and ecosystem health by investigating mass death events in gorals and serows in the Qinling Mountains. We conducted interviews with practitioners and local people in the central Qinling Mountains. From the same locations, we collected 24 cutaneous samples from various animals and surveillance data from infrared cameras. Pathological, parasitological and microbiological examinations of the samples were performed. Mite‐induced cutaneous lesions, mites and eggs were observed in samples from dead gorals and one dead serow but not in other species. Molecular analysis confirmed the mites to be S. scabiei and shared the same cox 1 genotype. The data obtained from the interviews and infrared cameras indicated that the death of wildlife was related to sarcoptic mange infection and that there had been a decrease in the goral population since the outbreak of the disease. We confirmed that sarcoptic mange was the major cause of the mass death events and may have spread from the western to eastern Qinling Mountains. Based on our findings, we propose several protection strategies to help preserve biodiversity in the Qinling Mountains.

show abstract

Section: Methodsmentioning

confidence: 99%

Sarcoptic mange is an emerging threat to biodiversity in the Qinling Mountains in China

Chen

Zhang

et al. 2022

Transbounding Emerging Dis

View full text Add to dashboard Cite

show abstract

“…Portions of cutaneous samples stored at -20°C were macerated in liquid nitrogen, and genomic DNA was extracted from each sample using a Universal Genomic DNA Extraction Kit (D2100, Solarbio, China) and subjected to PCR analysis. Fragments of S. scabiei cytochrome oxidase 1 (cox-1) (374 bp) and the second internal transcribed spacer gene (ITS2) (361 bp) were amplified (S2) (Fraser et al, 2018;Naz et al, 2020). The amplification products were sequenced by Sangon Biotech (Shanghai) Co., Ltd and identified by BLAST (NCBI).…”

Section: Molecular Assaysmentioning

confidence: 99%

Sarcoptic Mange is an Emerging Threat to Biodiversity in the Qinling Mountains in China

Chen

Zhang

et al. 2022

Preprint

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Sarcoptic mange, a disease caused by the burrowing mite Sarcoptes scabiei, is globally endemic and an emerging threat to wildlife. Although many studies have shown that wildlife diseases play key roles in biodiversity conservation, knowledge about sarcoptic mange is still insufficient. In this study, we aim to improve the understanding of the impacts of sarcoptic mange on wildlife populations, the mechanisms involved in its ecoepidemiology, and the associated risks to public and ecosystem health by investigating mass death events in gorals and serows in the Qinling Mountains. We conducted interviews with practitioners and local people in the central Qinling Mountains. From the same locations, we collected 24 cutaneous samples from various animals and surveillance data from infrared cameras. Pathological, parasitological and microbiological examinations of the samples were performed. Mite-induced cutaneous lesions, mites and eggs were observed in samples from dead gorals and one dead serow, but not in other species. Molecular analysis confirmed the mites to be S. scabiei that originated from rabbits. The data obtained from the interviews and infrared cameras indicated that the death of wildlife was related to sarcoptic mange infection and that there had been a decrease in the goral population since the outbreak of the disease. We confirmed that sarcoptic mange was the major cause of the mass death events and may have spread from the western to eastern Qinling Mountains. Based on our findings, we propose several protection strategies to help preserve biodiversity in the Qinling Mountains.

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Molecular Characterization of Agonoscena pistaciae Burckhardt and Lauterer (Hemiptera Psyllidae) Populations Spreading in Southeast Anatolia

2022

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Phylogenetic Analysis of Astigmatid Mites Sarcoptes scabiei and Dermatophagoides farinae using ITS-2 as a Genetic Marker

Cited by 3 publications

References 18 publications

Sarcoptic mange is an emerging threat to biodiversity in the Qinling Mountains in China

Sarcoptic mange is an emerging threat to biodiversity in the Qinling Mountains in China

Sarcoptic Mange is an Emerging Threat to Biodiversity in the Qinling Mountains in China

Molecular Characterization of Agonoscena pistaciae Burckhardt and Lauterer (Hemiptera Psyllidae) Populations Spreading in Southeast Anatolia

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