Phylogenetic Analysis of Aedes aegypti Based on Mitochondrial ND4 Gene Sequences in Almadinah, Saudi Arabia

Ali, Khalil H. Al; El-Badry, Ayman A.; Ali, Mouhanad Al; El‐Sayed, Wael S.; El-Beshbishy, Hesham A.

doi:10.15171/ijb.1329

Cited by 7 publications

(4 citation statements)

References 22 publications

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“…quinquefasciatus and Cx. pipiens, which are the main vectors of bancroftian filariasis, Wuchereria bancrofti worldwide including the Middle East countries, have been reported from the south-western districts of Saudi Arabia [6]. 3).…”

Section: Culex Quinquefasciatus (Figurementioning

confidence: 99%

“…Mosquito-borne diseases, i.e., malaria [1,2], filariasis [3], Rift Valley fever [4,5], West Nile virus [6], dengue, and others [7,8], pose potential public health threats in Saudi Arabia.…”

Section: Introductionmentioning

confidence: 99%

“…Several authors have investigated the distribution of mosquito species in Saudi Arabia [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

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Culex (Diptera: Culicidae) Mosquitoes in Jazan Region, Saudi Arabia, and Their Molecular Identification

Noureldin

Dafalla

Hakami

et al. 2021

International Journal of Zoology

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Morphological characteristics have been the gold standard method to identify mosquito species. However, morphological identification has many limitations including lack of expertise and damaging of external characters due to improper specimen handling. Therefore, we used the polymerase chain reaction technique (PCR) as an integrated tool to identify Culex mosquito species to establish a more precise and reliable identification system related to their spatial distribution in Jazan region. We identified Culex mosquito species and subspecies using taxonomic keys, and then we used the polymerase chain reaction technique (PCR) as an integrated tool to confirm and refine the list of Culex mosquito species in the region. Phylogenetic trees were constructed for the identified species, and their distinctive clustering was compared with their reference’s species in the GenBank. We identified 7026 adult Culex mosquitoes belonging to 4 species. Culex tritaeniorhynchus was the predominant species (45%), followed by Cx. quinquefasciatus (32%), then Culex sitiens (20%), and Cx. pipiens (3%). The most infested areas by Culex in the region were Gizan and Sabya. The PCR achieved 100% success in identifying the four Culex mosquito species. We also report the molecular identification of Cx. quinquefasciatus and Cx. pipiens species for the first time in Jazan region while the molecular identification of Cx. tritaeniorhynchus and Cx. sitiens was reported for the first time in Jazan region and the whole Saudi Arabia. This study utilized for the first time PCR to identify Culex mosquito species in Jazan region. The PCR is a complementary and integrated taxonomy-based identification tool for mosquito species. This integration has the capacity to promote and enhance vector surveillance and control programs, as well as defining the genetic diversity of species in the region.

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Section: Culex Quinquefasciatus (Figurementioning

confidence: 99%

“…Mosquito-borne diseases, i.e., malaria [1,2], filariasis [3], Rift Valley fever [4,5], West Nile virus [6], dengue, and others [7,8], pose potential public health threats in Saudi Arabia.…”

Section: Introductionmentioning

confidence: 99%

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Culex (Diptera: Culicidae) Mosquitoes in Jazan Region, Saudi Arabia, and Their Molecular Identification

Noureldin

Dafalla

Hakami

et al. 2021

International Journal of Zoology

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“…Microsatellite markers have been widely used to characterize genetic population structure in A. aegypti worldwide (Gloria‐Soria et al., 2016). In Saudi Arabia, only two studies from Madinah and Jeddah have addressed the population dynamics of A. aegypti using the mitochondrial cytochrome‐c‐oxidase subunit‐1 (CO1) and dehydrogenase subunit‐4 (ND4) (Ali et al., 2016; Khater et al., 2021) gene regions, but no finer scale genetic studies (i.e., using microsatellites or SNPs) have been conducted yet. Similarly, a study of the gene flow dynamics in southern and southwestern Saudi Arabia (i.e., Jazan and Sahil) is still absent (Mashlawi et al., 2022).…”

Section: Introductionmentioning

confidence: 99%

Microsatellite‐based analysis reveals Aedes aegypti populations in the Kingdom of Saudi Arabia result from colonization by both the ancestral African and the global domestic forms

Mashlawi,

Alqahtani,

Abuelmaali

et al. 2024

Evolutionary Applications

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The Aedes aegypti (Linnaeus, 1762) mosquito is the main vector of dengue, chikungunya and Zika and is well established today all over the world. The species comprises two forms: the ancestral form found throughout Africa and a global domestic form that spread to the rest of the tropics and subtropics. In Saudi Arabia, A. aegypti has been known in the southwest since 1956, and previous genetic studies clustered A. aegypti from Saudi Arabia with the global domestic form. The purpose of this study was to assess the genetic structure of A. aegypti in Saudi Arabia and determine their geographic origin. Genetic data for 17 microsatellites were collected for A. aegypti ranging from the southwestern highlands of Saudi Arabia on the border of Yemen to the north‐west in Madinah region as well as from Thailand and Uganda populations (as representatives of the ancestral African and global domestic forms, respectively). The low but significant level of genetic structuring in Saudi Arabia was consistent with long‐distance dispersal capability possibly through road connectivity and human activities, that is, passive dispersal. There are two main genetic groupings in Saudi Arabia, one of which clusters with the Ugandan population and the other with the Thailand population with many Saudi Arabian individuals having mixed ancestry. The hypothesis of genetic admixture of the ancestral African and global domestic forms in Saudi Arabia was supported by approximate Bayesian computational analyses. The extent of admixture varied across Saudi Arabia. African ancestry was highest in the highland area of the Jazan region followed by the lowland Jazan and Sahil regions. Conversely, the western (Makkah, Jeddah and Madinah) and Najran populations corresponded to the global domesticated form. Given potential differences between the forms in transmission capability, ecology and behaviour, the findings here should be taken into account in vector control efforts in Saudi Arabia.

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Screening of the normal bacterial flora in the gut of Aedes aegypti Mosquito in Saudi Arabia

Alghamdi

Farrag

Abdel‐Gaber

et al. 2023

Journal of King Saud University - Science

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Phylogenetic Analysis of Aedes aegypti Based on Mitochondrial ND4 Gene Sequences in Almadinah, Saudi Arabia

Cited by 7 publications

References 22 publications

Culex (Diptera: Culicidae) Mosquitoes in Jazan Region, Saudi Arabia, and Their Molecular Identification

Culex (Diptera: Culicidae) Mosquitoes in Jazan Region, Saudi Arabia, and Their Molecular Identification

Microsatellite‐based analysis reveals Aedes aegypti populations in the Kingdom of Saudi Arabia result from colonization by both the ancestral African and the global domestic forms

Screening of the normal bacterial flora in the gut of Aedes aegypti Mosquito in Saudi Arabia

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