Phylogenetic Analysis of Acute Bee Paralysis Virus Strains

Bakonyi, Tamás; Grabensteiner, Elvira; Kolodziejek, Jolanta; Rusvai, Miklós; Topolska, G.; Ritter, W.; Nowotny, Norbert

doi:10.1128/aem.68.12.6446-6450.2002

Cited by 66 publications

(48 citation statements)

References 24 publications

(29 reference statements)

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“…Whether these differences are enough to warrant separate nomenclature for these viruses remains to be decided. Both the nucleotide and amino acid differences between DWV, KV, and VDV-1 are very much in line with the variability found among strains of other bee viruses (1,5,11,24,36). Furthermore, in light of what has been known about DWV for several decades, neither KV nor VDV-1 is particularly unique.…”

Section: Discussionmentioning

confidence: 55%

Molecular and Biological Characterization of Deformed Wing Virus of Honeybees ( Apis mellifera L.)

Lanzi

Miranda

Boniotti

et al. 2006

J Virol

298

370

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Deformed wing virus (DWV) of honeybees (Apis mellifera) is closely associated with characteristic wing deformities, abdominal bloating, paralysis, and rapid mortality of emerging adult bees. The virus was purified from diseased insects, and its genome was cloned and sequenced. The genomic RNA of DWV is 10,140 nucleotides in length and contains a single large open reading frame encoding a 328-kDa polyprotein. The coding sequence is flanked by a 1,144-nucleotide 5 nontranslated leader sequence and a 317-nucleotide 3 nontranslated region, followed by a poly(A) tail. The three major structural proteins, VP1 (44 kDa), VP2 (32 kDa), and VP3 (28 kDa), were identified, and their genes were mapped to the N-terminal section of the polyprotein. The C-terminal part of the polyprotein contains sequence motifs typical of well-characterized picornavirus nonstructural proteins: an RNA helicase, a chymotrypsin-like 3C protease, and an RNA-dependent RNA polymerase. The genome organization, capsid morphology, and sequence comparison data indicate that DWV is a member of the recently established genus Iflavirus.Deformed wing virus (DWV) is the one of the main viruses associated with the collapse of honeybee (Apis mellifera L.) colonies due to infestation with the ectoparasitic mite Varroa destructor (4,8,12). The virus was first isolated from a sample of symptomatic honeybees from Japan in the early 1980s and is currently distributed worldwide, wherever varroa mites are found (2, 18). A recent survey of adult bee populations detected DWV in over 90% of French apiaries (66) and in 100% of mite samples. The incidence was slightly reduced when pupal samples were analyzed, especially in the spring (66). DWV has also been detected by serology in the dwarf bee A. florae Fabr. (F. R. Hunter-Fujita, M. S. Mossadegh, and B. V. Ball, Abstr. 36th "Apimondia" Int. Apic. Congr., abstr. 230, 1999) and in the Asian honeybee A. cerana Fabr. (2) and by reverse transcriptase (RT) PCR in bumblebees (29). It is serologically related to Egypt bee virus (8, 9, 13), first isolated in 1977 from infected adults from Egypt (10). Typical symptoms of deformed wing disease are vestigial and crumpled wings, bloated abdomens, paralysis, and a severely shortened adult life span for emerging worker and drone bees (44). For a long time it was believed that these symptoms were due to the feeding activity of the mites (23, 42, 72) until it was shown that, in diseased colonies, deformed bees could emerge from cells not parasitized by varroa mites (49, 55) and that the symptoms can persist in the absence of mites (13). The symptoms are perfectly correlated with the presence of large amounts of DWV, as well as with the reduced virus titers and lower prevalence found in asymptomatic bees from the same colonies (17,19,20,54,67,68). The combination of mites and virus causes immunosuppression in the bees and increased susceptibility to other opportunistic pathogens (76), leading to a progressive reduction in colony performance and a complex disease profile at colony level...

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Section: Discussionmentioning

confidence: 55%

Molecular and Biological Characterization of Deformed Wing Virus of Honeybees ( Apis mellifera L.)

Lanzi

Miranda

Boniotti

et al. 2006

J Virol

298

370

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“…This virus has been reported in other South America countries, such as Argentina, Brazil, and Uruguay (Antúnez et al, 2005;Teixeira et al, 2008;Reynaldi et al, 2010). The Chilean isolate nucleotide sequence had a high percentage of similarity (in the Capsid protein gene) to Brazilian and Uruguayan isolates (97% and 93%, respectively) (there is no published data on the ABPV sequence from Argentina), confirming that the ABPV capsid polyprotein gene is a relatively conserved genomic region (Bakonyi et al, 2002). The proximity of Brazil and Uruguay to Chile suggests they are origin of the virus in Chile.…”

Section: Discussionmentioning

confidence: 68%

Prevalence and phylogenetic analysis of honey bee viruses in the Biobío Region of Chile and their association with other honey bee pathogens

Rodríguez¹,

Vargas²,

Antúnez

et al. 2014

Chilean J. Agric. Res.

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Different episodes of mortalities of honey bee (Apis mellifera L.) colonies have been associated with the presence of honey bee pathogens. Since the Biobío Region has among the highest number of apiaries in Chile, the aim of the present study was to identify viruses in the Region affecting honey bees, evaluate their relation to other pathogens, and conduct a phylogenetic analysis. Pupae and adult bees were collected from 60 apiaries of Apis mellifera L. in the Biobío Region over 2 yr. RNA viruses were detected by reverse transcription PCR (RT-PCR), and Acarapis woodi, Nosema spp., and Varroa destructor via PCR. Three viruses were detected: Acute bee paralysis virus (ABPV), Black queen cell virus (BQCV) and Deformed wing virus (DWV) in 2%, 10%, and 42% of the apiaries, respectively. No statistical correlation was observed between the presence of the different viruses, V. destructor, A. woodi, and the two Nosema species, and the bee development stages. One year after the first sampling, DWV and BQCV were detected mainly in foraging adult bee samples. Three percent of the apiaries were infected with N. apis and 18% with N. ceranae, 5% were positive for V. destructor, while A. woodi was not detected. PCR products were sequenced and compared to the Genbank database. Chilean sequences of ABPV, BQCV, and DWV showed high percentages of similarity to other isolates in South America.

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“…3C). Among them, 11 sequences were IGR-IRES elements in distinct dicistroviruses and 10 sequences were those of geographic isolates of ABPV (Bakonyi et al 2002). The parameter A1 identified the 3Ј end of the IGR-IRESs of dicistroviruses exactly, except for that of TrV, which had C at the 3Ј end of p6 and G at the 5Ј end of the capsid coding region.…”

Section: Computational Search For Ires Elementsmentioning

confidence: 99%

Structural variant of the intergenic internal ribosome entry site elements in dicistroviruses and computational search for their counterparts

Hatakeyama¹,

Shibuya²,

Nishiyama³

et al. 2004

RNA

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The intergenic region (IGR) located upstream of the capsid protein gene in dicistroviruses contains an internal ribosome entry site (IRES). Translation initiation mediated by the IRES does not require initiator methionine tRNA. Comparison of the IGRs among dicistroviruses suggested that Taura syndrome virus (TSV) and acute bee paralysis virus have an extra side stem loop in the predicted IRES. We examined whether the side stem is responsible for translation activity mediated by the IGR using constructs with compensatory mutations. In vitro translation analysis showed that TSV has an IGR-IRES that is structurally distinct from those previously described. Because IGR-IRES elements determine the translation initiation site by virtue of their own tertiary structure formation, the discovery of this initiation mechanism suggests the possibility that eukaryotic mRNAs might have more extensive coding regions than previously predicted. To test this hypothesis, we searched full-length cDNA databases and whole genome sequences of eukaryotes using the pattern matching program, Scan For Matches, with parameters that can extract sequences containing secondary structure elements resembling those of IGR-IRES. Our search yielded several sequences, but their predicted secondary structures were suggested to be unstable in comparison to those of dicistroviruses. These results suggest that RNAs structurally similar to dicistroviruses are not common. If some eukaryotic mRNAs are translated independently of an initiator methionine tRNA, their structures are likely to be significantly distinct from those of dicistroviruses.

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Phylogenetic Analysis of Acute Bee Paralysis Virus Strains

Cited by 66 publications

References 24 publications

Molecular and Biological Characterization of Deformed Wing Virus of Honeybees ( Apis mellifera L.)

Molecular and Biological Characterization of Deformed Wing Virus of Honeybees ( Apis mellifera L.)

Prevalence and phylogenetic analysis of honey bee viruses in the Biobío Region of Chile and their association with other honey bee pathogens

Structural variant of the intergenic internal ribosome entry site elements in dicistroviruses and computational search for their counterparts

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