Phylogenetic analysis, biofilm formation, antimicrobial resistance and relationship between these characteristics in Uropathogenic Escherichia coli

Talieh Mostaghimi,; Pournajaf, Abazar; Bijani, Ali; Mohammadi, Mohsen; Rajabnia, Mehdi; Halaji, Mehrdad

doi:10.1007/s11033-023-09031-x

Cited by 1 publication

(2 citation statements)

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“…Much higher frequencies of hlyCA (17.0%) and cnf (16.4%) were found among UPEC than PEC in this study. However, those from UPEC observed in this study were higher than in the previous study from Thailand (7% and 1%, respectively) [ 40 ]. These genes are involved in tissue damage and have been linked to patients with pyelonephritis rather than cystitis [ 46 , 47 ].…”

Section: Discussioncontrasting

confidence: 88%

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Phylogenetic diversity and virulence gene characteristics of Escherichia coli from pork and patients with urinary tract infections in Thailand

Ketkhao,

Utrarachkij,

Parikumsil

et al. 2024

PLoS ONE

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Extraintestinal pathogenic Escherichia coli (ExPEC), especially uropathogenic E. coli (UPEC) are responsible for urinary tract infections (UTIs), while diarrheagenic E. coli (DEC) cause foodborne illnesses. These pathogenic E. coli are a serious threat to human health and a public concern worldwide. However, the evidence on pork E. coli (PEC) harboring UPEC virulence-associated genes is currently limited. Therefore, this study aimed to determine the phylogroups, virulence genes, and their association between PEC and UPEC from UTI patients. In this study, 330 E. coli were obtained from archived stock culture isolated from pork (PEC; n = 165) and urine of patients with UTIs (UPEC; n = 165) during 2014–2022. Phylogroups, UPEC- and diarrheagenic E. coli (DEC) associated virulence genes were assessed using PCR assays. The results showed that phylogroups A (50.3%), and B1 (32.1%) were commonly found among PEC whereas phylogroups B2 (41.8%), and C (25.5%) were commonly detected in the UPEC. PEC and UPEC carried similar virulence-associated genes with different percentages. The most frequent UPEC virulence-associated gene among UPEC, and PEC strains was fimH, (93.3%, and 92.1%), followed by iucC (55.2%, and 12.7%), papC (21.8%, and 4.2%), afaC (22.4%, and 0%), hlyCA (17%, and 0.6%), cnf (16.4%, and 0.6%), and sfa/focDE (8.5%, and 4.8%). Additionally, 6 of 27 UPEC virulence-associated gene patterns were found in both PEC and UPEC strains regardless of phylogroups. Furthermore, the DEC virulence-associated genes were found in only 3 strains, one from PEC harboring eae, and two from UPEC carried fimH-bfpA or afaC-CVD432 indicating hybrid strains. Cluster analysis showed a relationship between PEC and UPEC strains and demonstrated that PEC harboring UPEC virulence-associated genes in pork may be associated with UPEC in humans. Food safety and hygiene practices during pork production chain are important procedures for minimizing cross-contamination of these strains that could be transmitted to the consumers.

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Section: Discussioncontrasting

confidence: 88%

“…It is known that E . coli from phylogroups A and B1 are often commensal [ 28 ], while human UPEC strains causing UTIs belong to phylogroups B2 and D [ 39 , 40 ]. Therefore, most of the PEC and UPEC in this study were commensal and ExPEC, respectively.…”

Section: Discussionmentioning

confidence: 99%