Phylogenetic Analysis Based on DNA Barcoding and Genetic Diversity Assessment of Morinda officinalis How in Vietnam Inferred by Microsatellites

Abstract

Morinda officinalis How. is well-known as a valuable medicinal plant found in some regions of Vietnam. This species is mainly used for treating male impotence, irregular menstruation, and rheumatoid arthritis. This study aimed to identify the species of and genetic diversity in three M. officinalis populations: one each in Quang Binh (QB), Thua Thien Hue (TTH), and Quang Nam (QN). In this study, four DNA barcoding markers (ITS1, ITS2, matK, and rbcL) were used to identify the species and 22 microsatellite markers were applied for population structure and diversity analyses. The results showed that the sequences of gene regions studied in M. officinalis had a high similarity (>95%) to the ITS1, ITS2, matK, and rbcL sequences of M. officinalis on BLAST. Of the four DNA barcoding markers used, ITS1 and ITS2 showed higher efficiency in DNA amplification of M. officinalis. From this study, 27 GenBank codes were published on BLAST. The results also revealed high levels of genetic diversity in populations. The average observed and expected heterozygosity values were HO = 0.513 and HE = 0.612, respectively. The average FST value was 0.206. Analysis of molecular variance (AMOVA) showed 70% variation within populations and 30% among populations. The population structure of M. officinalis inferred in STRUCTURE revealed that the optimum number of genetic groups for the admixture model was K = 2. These findings provided vital background information for future studies in the conservation of M. officinalis in both ex situ and in situ plans.

Delineating the Area for Sustainable Cultivation of Morinda officinalis Based on the MaxEnt Model

“…In contemporary times, the escalating demand for Morinda officinalis has resulted in their excessive exploitation, consequently depleting the available raw materials. However, current research in the academic community on Morinda officinalis mainly focuses on its chemical composition [2][3][4], genetic diversity [5], etc. Only a small number of scholars have conducted research on its germplasm resource protection [6], and there is almost no related research on plant ecology.…”

Anti-inflammatory and Antioxidant Activities of Root Extract of Morinda officinalis from Quang Binh Province, Vietnam

“…[11][12][13][14] The antioxidant, anti-chromosomal mutagenesis, anticancer, and analgesic properties of M. officinalis iridoids have also been reported. [15][16][17] In Vietnam, the focus on M. officinalis involves its development in breeding procedure 3 , cultivation 2 , and evaluation of species genetic diversity 4,18 , while assessing the species' active compounds has not been investigated or given attention. This study aimed to evaluate the biological potential of the root extract of M. officinalis in line with our goal of finding bioactive natural compounds from traditional therapeutic plants.…”