Phylogenetic Analysis and Sequencing of the Mitochondrial Cytochrome Oxidase Sub Unit I (Coi) of White Backed Plant Hopper, Sogatella Furcifera (Horvath)

Abstract

The family Delphacidae is the largest and most economically important one amongst the hoppers, Fulgoroidea. It has representatives in tropical and subtropical regions that occur in a variety of environments, and its known fossil records dates back to Jurassic period. Sogatella furcifera, the white backed plant hopper is a serious pest of rice in many Asian countries. We have performed the phylogenetic reconstruction of the S. furcifera using the mitochondrial cytochrome oxidase subunit I (COI) gene. Phylogenetic reconstructions of the COI regions were done using Neighbor joining method. This study gives a clear picture of the mitochondrial genome of the insect and gives the phylogenetics of S. furcifera which help us to develop a sustainable and accurate strategy for management of the pest.

Molecular Phylogenetic Study of Periplaneta Fuliginosa From Lakshadweep Islands, India Using Cytochrome Oxidase Subunit Gene Sequence

“…These are the most widely used gene for molecular barcoding and phylogeny analysis of organisms especially higher eukaryotes for its high level of sequence variation compared to other region of mitochondrial DNA. Molecular phylogenetic analysis using partial mitochondrial COI gene sequences were reported in a range of insect taxa like dipterans 13,14 , lepidopterans 10,15 , heteropterans 16 and hymenopterans 5,17 .…”

Molecular Phylogenetic Analysis of Asota Orbona and Asota Caricae (Lepidoptera: Erebidae) Using Mitochondrial Coi Gene

“…A major feature of DNA barcoding is that it allows prompt identification of pest during young instars 2,3. The mitochondrial DNA has been extensively analysed and proven to be an important tool in species delimitation as it possesses biological properties making it suitable as a marker for molecular biodiversity. Fragment size of mitochondrial cytochrome oxidase subunit I (COI) gene has been shown to provide high resolution to identify cryptic species, thereby increasing taxonomy-based biodiversity estimates and its usefulness has been confirmed for identifying Coleoptera 4 , Diptera 5,6 , Odonata 7 , Hemiptera 8,9 , Hymenoptera 10 and Lepidoptera 11,12 . DNA barcoding has proved to be a versatile tool with a variety of applications, for example, by facilitating the association between different developmental stages in insects.…”