Phylogenetic Analysis and Lipoxygenase (LOX) Gene Family Variation in The Pistachio

Abstract

Lipoxygenases (LOX) gene family is a type of nonheme iron-containing dioxygenases, which has a very important aspect in plant development and fruit quality. LOX gene, which is responsible for lipid oxidation, the main role for the biosynthesis pathway of unsaturated fatty acids. Although some studies have investigated the LOX gene family in several species including arabidopsis, soybean, peanut and apple, there is no information from Pistachio; and the phylogeny of this gene family in the Pistacia genus is still not determined. In this study, Arabidopsis thaliana LOX1 gene (NCBI Reference Sequence: NM_104376.3) was selected and used as a query sequence for performing a BLASTN search. Among all sequence query which was found by NCBI platform, 9 sequences were selected for further analysis. Phylogenetic tree of full-length LOX gene sequences from the Pistacia genus was constructed using the Maximum Likelihood method with MEGA software. By using phylogenic analysis, we identified variations in gene structure and revealed the phylogenetic evolutionary relationship of this gene family. Additionally, this may serve as a reference value for assessing the genetic relationships among various LOX genes in Pistacia genus species. This variations provides us the possibility the design the primer to achieve us to find the exact LOX gene in Pistacia genus and future research on the evolutionary history and transgenic research on LOX genes.