Phylogenetic analyses of Japanese golden chanterelles and a new species description, Cantharellus anzutake sp. nov.

Abstract

The Japanese golden chanterelle commonly identified as Cantharellus cibarius was sampled 3 in a broad range of forest vegetation. A total of 90 fresh and 11 herbarium specimens were 4 examined microscopically, subjected to sequencing analysis of their nuclear ribosomal RNA 5 (rDNA) and tef-1 genes, and their characteristics were compared with those of European C. 6 cibarius. Based on morphological and ecological characteristics, basidioma samples from 7 Japan were divided into four species. While specimens of Cantharellus sp. 4 from Hokkaido 8 Island were included in the European C. cibarius clade phylogenetically, the other three 9 species formed three unique clades. Among these, Cantharellus anzutake sp. nov. is sister to 10 the clade of C. cibarius and was widely sampled from the northern limit of Honshu Island to 11 the southern limit of Kumejima Island in Ryukyu Islands. Although C. anzutake was 12 morphologically similar to C. cibarius, the two species were phylogenetically distinct. Other 13 morphologically similar but genetically distinct chanterelle species from India exhibited 14 macroscopic and microscopic differences compared with C. anzutake.

New Cantharellus species from South Korea

“…Hence, positive identification frequently requires molecular sequence data. Our identification is here based on the high quality ITS sequence we obtained for VA 16.142 and which is identical to the one deposited for the C. anzutake holotype (GenBank LC085359 , similarity 100% for 100% coverage); both these ITS differ from other yellow species of chanterelles described from Asia by a ca 100 bp deletion in the ITS1 region ( Ogawa et al 2018 ).…”

“…In addition, identical ITS sequence data also document the presence, in South Korea, of the recently described C. anzutake from Japan, a species belonging to subg. Cantharellus and based on a 100 base pair deletion in the internal transcribed spacer 1 (ITS1) of the rDNA (Ogawa et al 2018). Obtained tef-1 sequence data from all of our recent collections of chanterelles in South Korea could not confirm the presence of any of the European or North American Cantharellus previously reported from South Korea (Park and Lee 1991;Kim 2004;Kim et al 2006;Lee 2011) nor any of the chanterelles already described from India (Das et al 2015;Kumari et al 2011Kumari et al , 2013, neighbouring China (Shao et al 2011(Shao et al , 2014(Shao et al , 2016aTian et al 2012;, Japan (Suhara and Kurogi 2015;Ogawa et al 2018) or Malaysia (Corner 1966;Eyssartier et al 2009) Materials and methods…”

“…in Korea ( Antonín et al 2017 ; Buyck et al 2018 ), we have reviewed the still limited taxonomic knowledge on Cantharellus species in Asia. During the past two years two more new chanterelles have been described from Asia: C. anzutake W. Ogawa, N. Endo, M. Fukuda and A. Yamada from Japan ( Ogawa et al 2018 ) and C. hainanensis N.K. Zeng, Zhi Q. Liang & S. Jiang from China ( An et al 2017 ).…”

Figure 8 from: Buyck B, Hofstetter V, Ryoo R, Ka K-H, Antonín V (2020) New Cantharellus species from South Korea. MycoKeys 76: 31-47. https://doi.org/10.3897/mycokeys.76.58179

“…Hence, positive identification frequently requires molecular sequence data. Our identification is here based on the high quality ITS sequence we obtained for VA 16.142 and which is identical to the one deposited for the C. anzutake holotype (GenBank LC085359, similarity 100% for 100% coverage); both these ITS differ from other yellow species of chanterelles described from Asia by a ca 100 bp deletion in the ITS1 region (Ogawa et al 2018).…”

Figure 6 from: Buyck B, Hofstetter V, Ryoo R, Ka K-H, Antonín V (2020) New Cantharellus species from South Korea. MycoKeys 76: 31-47. https://doi.org/10.3897/mycokeys.76.58179

“…in Korea (Antonín et al 2017;Buyck et al 2018), we have reviewed the still limited taxonomic knowledge on Cantharellus species in Asia. During the past two years two more new chanterelles have been described from Asia: C. anzutake W. Ogawa, N. Endo, M. Fukuda and A. Yamada from Japan (Ogawa et al 2018) and C. hainanensis N.K. Zeng, Zhi Q. Liang & S. Jiang from China .…”