Phyllostachys edulis extract induces apoptosis signaling in osteosarcoma cells, associated with AMPK activation

Abstract

ObjectiveBamboo is distributed worldwide, and its different parts are used as foods or as a traditional herb. Recently, antitumoral effects of bamboo extracts on several tumors have been increasingly reported; however, antitumoral activity of bamboo extracts on osteosarcoma remains unclear. In the present study, we investigated effects of an aqueous Phyllostachys edulis leaf extract (PEE) on osteosarcoma cells and the underlying mechanism of inhibition.MethodsThe growth of human osteosarcoma cell lines 143B and MG-63 and lung fibroblast MRC-5 cells was determined by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Apoptosis was demonstrated using TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) assay and flow cytometric analysis. Phosphorylation and protein levels were determined by immunoblotting.ResultsAfter treatment with PEE, viability of 143B and MG-63 cells was dose-dependently reduced to 36.3%±1.6% of control values, which were similar to AICAR (5-aminoimidazole-4-carboxamide 1-β-D-ribofuranoside) treatments. In parallel, ratios of apoptotic cells and cells in the sub-G1 phase were significantly increased. Further investigation showed that PEE treatments led to activation of caspase cascades and changes of apoptotic mediators Bcl2, Bax, and p53. Consistently, our results revealed that PEE activated adenosine monophosphate-activated protein kinase (AMPK) signaling, and the AMPK activation was associated with the induction of apoptotic signaling.ConclusionOur results indicated that PEE suppressed the growth of 143B and MG-63 cells but moderately affected MRC-5 cells. PEE-induced apoptosis may attribute to AMPK activation and the following activation of apoptotic signaling cascades. These findings revealed that PEE possesses antitumoral activity on human osteosarcoma cells by manipulating AMPK signaling, suggesting that PEE alone or combined with regular antitumor drugs may be beneficial as osteosarcoma treatments.

Chemical Constituent Profiling of Phyllostachys heterocycla var. Pubescens with Selective Cytotoxic Polar Fraction through EGFR Inhibition in HepG2 Cells

“…Previous studies proved the potential of vigorous bamboo leaf extract as a tumor suppressive and cancer-preventive food supplement [ 8 ]. In addition, another species, Phyllostachys edulis, extract proved to induce apoptosis signaling in osteosarcoma cells, associated with AMPK activation [ 9 ]. The present study deals with Phyllostachys heterocycla var.…”

Ethanol extract of Kalopanax septemlobus leaf induces caspase-dependent apoptosis associated with activation of AMPK in human hepatocellular carcinoma cells

“…For example, metformin, a common diabetes drug, shows anticancer effects against HCC by activation of AMPK (27,(34)(35)(36). Similarly, a number of medicinal herb extracts exert anticancer effects through activation of AMPK-dependent cell death pathways (29,31,(37)(38)(39)(40).…”

Etnobotánica urbana: el conocimiento botánico local sobre las plantas alimenticias y medicinales, y sus usos, en la conurbación Buenos Aires-La Plata

“…M: antioxidante, digestivo, hipotensor, antiinflamatorio, vulnerario. E: antiinflamatorio, hipotensor, protector cardiovascular, antiarterioesclerosis, antioxidante, antidiabético, anticáncer (Lin et al 2008;Zhang et al 2011;Upadhyay & Mohan Rao 2013;Chou et al 2014;Pang & Panee 2016). , potenciador de la circulación, cardioprotector, analgésico, inmunomodulador, antiviral, antimicrobiano, antialérgico, antiinflamatorio, hipoglucémico, antidiabético, hepatoprotector, hipocolesterolémico, antiarterioesclerosis, anticáncer, antitusivo (Lam et al 2000;Hwang et al 2012;Adams & Lien 2013;Meng et al 2014;, anti-mastitis , antiartrítico (Jung et al 2001), anti-colitis (Haarberg et al 2015), antiestrogénico (Collins et al 2009), potenciador cognitivo, anti-Alzheimer, anti-esquizofrenia (Park et al 2010(Park et al , 2015, ansiolítico (Kwon et al 2014).…”