Phyllosphere microbiome: Diversity and functions

Bashir, Iqra; War, Aadil Farooq; Rafiq, Iflah; Reshi, Zafar A.; Rashid, Irfan; Shouche, Yogesh S.

doi:10.1016/j.micres.2021.126888

Cited by 112 publications

(108 citation statements)

References 268 publications

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“…Fungal communities play significant roles in decomposition, pathogenesis and plant nutrient uptake [ 12 , 13 , 14 ], and most current research is interested in the fungal communities of the rhizosphere (the root–soil interface). On the other hand, leaf-associated fungi are abundant, and community compositions are often highly diverse [ 15 , 16 ] and can determine plant health and productivity. For example, fungal pathogens can cause devastating plant diseases, such as rice blast, anthracnose, leaf spot, soybean rust, wilt, blight, mildew and dieback in multiple crop species [ 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

Leaf-Associated Epiphytic Fungi of Gingko biloba, Pinus bungeana and Sabina chinensis Exhibit Delicate Seasonal Variations

Bao

Sun

Liu

et al. 2022

JoF

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Plant-leaf surface on Earth harbors complex microbial communities that influence plant productivity and health. To gain a detailed understanding of the assembly and key drivers of leaf microbial communities, especially for leaf-associated fungi, we investigated leaf-associated fungal communities in two seasons for three plant species at two sites by high-throughput sequencing. The results reveal a strong impact of growing season and plant species on fungal community composition, exhibiting clear temporal patterns in abundance and diversity. For the deciduous tree Gingko biloba, the number of enriched genera in May was much higher than that in October. The number of enriched genera in the two evergreen trees Pinus bungeana and Sabina chinensis was slightly higher in October than in May. Among the genus-level biomarkers, the abundances of Alternaria, Cladosporium and Filobasidium were significantly higher in October than in May in the three tree species. Additionally, network correlations between the leaf-associated fungi of G. biloba were more complex in May than those in October, containing extra negative associations, which was more obvious than the network correlation changes of leaf-associated fungi of the two evergreen plant species. Overall, the fungal diversity and community composition varied significantly between different growing seasons and host plant species.

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Section: Introductionmentioning

confidence: 99%

Leaf-Associated Epiphytic Fungi of Gingko biloba, Pinus bungeana and Sabina chinensis Exhibit Delicate Seasonal Variations

Bao

Sun

Liu

et al. 2022

JoF

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“…Most likely, this is related to the fact that the phyllosphere, including leaves, represents a fluctuating, unstable and relatively harsh environment. Microbial communities associated with the phyllosphere are exposed to various environmental factors, such as ultraviolet radiations, temperature, and humidity fluctuations, constricting the growth and activity of most microorganisms [ 10 ].…”

Section: Discussionmentioning

confidence: 99%

“…The phyllosphere represents a fluctuating, unstable and relatively harsh environment. Microbial communities associated with the phyllosphere are exposed to various environmental factors, i.a., ultraviolet radiations, temperature and humidity fluctuations, with negative impacts on the growth of most microorganisms [ 10 ]. Nevertheless, this environment comprises a living habitat for lots of various communities of microorganisms well adapted to these unfavourable conditions [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Microbial Community, Metabolic Potential and Seasonality of Endosphere Microbiota Associated with Leaves of the Bioenergy Tree Paulownia elongata × fortunei

Woźniak

Gałązka

Marzec-Grządziel

et al. 2022

IJMS

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The microbial structure and metabolic function of plant-associated endophytes play a key role in the ecology of various environments, including trees. Here, the structure and functional profiles of the endophytic bacterial community, associated with Paulownia elongata × fortunei, in correlation with seasonality, were evaluated using Biolog EcoPlates. Biolog EcoPlates was used to analyse the functional diversity of the microbiome. The total communities of leaf endophyte communities were investigated using 16S rRNA V5–V7 region amplicon deep sequencing via Illumina MiSeq. Community level physiological profiling (CLPP) analysis by the Biolog EcoPlate™ assay revealed that the carboxylic acids (19.67–36.18%) and amino acids (23.95–35.66%) were preferred by all by all communities, whereas amines and amides (0.38–9.46%) were least used. Seasonal differences in substrate use were also found. Based on the sequencing data, mainly phyla Proteobacteria (18.4–97.1%) and Actinobacteria (2.29–78.7%) were identified. A core microbiome could be found in leaf-associated endophytic communities in trees growing in different locations. This work demonstrates the application of Biolog EcoPlates in studies of the functional diversity of microbial communities in a niche other than soil and shows how it can be applied to the functional analyses of endomicrobiomes. This research can contribute to the popularisation of Biolog EcoPlates for the functional analysis of the endomicrobiome. This study confirms that the analysis of the structure and function of the plant endophytic microbiome plays a key role in the health control and the development of management strategies on bioenergy tree plantations.

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“…Although the methodology for isolating single phages is time-consuming and biased towards speci c, plaque-forming vira, it is an important task that will further promote the study of metaviromes [2,3]. Erwinia is a major bacterial genus, its members are often present as commensals and pathogens in the phyllosphere [4]. Erwinia billingiae is often identi ed as an epiphyte that can compete as an antagonist against the plant pathogen E. amylovora, the causal agent of re blight in apple and pear trees, but E. billingiae has also been observed to be pathogenic towards plants [5][6][7][8][9].…”

Section: Main Textmentioning

confidence: 99%

Three novel Erwinia billingiae phages represent three new genera isolated from organic waste

Alanin

Olsen

Djurhuus

et al. 2022

Preprint

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Despite the ecological significance of viral communities, phages remain understudied. Current genomic databases lack high-quality phage genomes linked to specific bacteria. Bacteria of the Erwinia genus are known to colonize the phyllosphere of plants, both as commensals and pathogens. We isolated three Erwinia billingiae phages; Zoomie, Pecta, and Snitter from organic household waste. Based on sequence similarity to their closest relatives, we propose that they represent three new genera “Pectavirus” within the Zobellviridae family, “Snittervirus” in the subfamily Tempevirinae, family Drexlerviridae, and “Zoomievirus” within the Autographviridae family, which may constitute a yet undefined sub-family together with the genus Limelightvirus.

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Phyllosphere microbiome: Diversity and functions

Cited by 112 publications

References 268 publications

Leaf-Associated Epiphytic Fungi of Gingko biloba, Pinus bungeana and Sabina chinensis Exhibit Delicate Seasonal Variations

Leaf-Associated Epiphytic Fungi of Gingko biloba, Pinus bungeana and Sabina chinensis Exhibit Delicate Seasonal Variations

Microbial Community, Metabolic Potential and Seasonality of Endosphere Microbiota Associated with Leaves of the Bioenergy Tree Paulownia elongata × fortunei

Three novel Erwinia billingiae phages represent three new genera isolated from organic waste

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