Phyllosphere-associated microbiota in built environment: Do they have the potential to antagonize human pathogens?

Wicaksono, Wisnu Adi; Reisenhofer-Graber, Tamara; Erschen, Sabine; Kusstatscher, Peter; Berg, Christian; Krause, Robert; Cernava, Tomislav; Berg, Gabriele

doi:10.1016/j.jare.2022.02.003

Cited by 13 publications

(5 citation statements)

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“…Fluctuations in UV radiation and temperature as well as water and nutrients heterogeneous result in an ephemeral, and unstable biotope for phyllosphere inhabitants ( Vorholt, 2012 ). Apart from environmental variables and geospatial dispersion patterns of microorganisms ( Bokulich et al, 2014 ; Wicaksono et al, 2023 ), factors related to phenotypic traits such as leaf morphology ( Hunter et al, 2010 ; Kusstatscher et al, 2020 ), cuticle synthesis ( Bodenhausen et al, 2014 ; Ritpitakphong et al, 2016 ) or volatile and hormone signaling ( Vorholt, 2012 ) account for the selection of a distinct microbiome. Moreover, previous investigations have shown a strong impact of urban intensity ( Laforest-Lapointe et al, 2017 ), crop management practices ( Grube et al, 2011 ; Knorr et al, 2019 ; Chen et al, 2021 ), and season ( Redford and Fierer, 2009 ; Rastogi et al, 2012 ) on the phyllosphere microbiome.…”

Section: Introductionmentioning

confidence: 99%

The mature phyllosphere microbiome of grapevine is associated with resistance against Plasmopara viticola

Wicaksono

Morauf²,

Müller

et al. 2023

Front. Microbiol.

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Phyllosphere microbiota represents a substantial but hardly explored reservoir for disease resistance mechanisms. The goal of our study was to understand the link between grapevine cultivars susceptibility to Plasmopara viticola, one of the most devastating leaf pathogens in viticulture, and the phyllosphere microbiota. Therefore, we analyzed a 16S rRNA gene library for the dominant phyllosphere bacterial phyla Alphaproteobacteria of seven Vitis genotypes at different developmental stages, i.e., flowering and harvesting, via amplicon sequencing. Young leaves had significantly higher Alphaproteobacterial richness and diversity without significant host-specificity. In contrast, the microbial communities of mature leaves were structurally distinct in accordance with P. viticola resistance levels. This statistically significant link between mature bacterial phyllosphere communities and resistant phenotypes was corroborated by beta diversity metrics and network analysis. Beyond direct host-driven effects via the provision of microhabitats, we found evidence that plants recruit for specific bacterial taxa that were likely playing a fundamental role in mediating microbe-microbe interactions and structuring clusters within mature communities. Our results on grape-microbiota interaction provide insights for targeted biocontrol and breeding strategies.

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Section: Introductionmentioning

confidence: 99%

The mature phyllosphere microbiome of grapevine is associated with resistance against Plasmopara viticola

Wicaksono

Morauf²,

Müller

et al. 2023

Front. Microbiol.

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“…In agriculture, biocontrol has been rigorously investigated as a replacement for pesticidal, antimicrobial, and antifungal compounds used to eliminate disease-causing organisms [ 74 , 75 ]. As many plant-associated microbes are naturally antagonistic towards human-associated pathogens [ 76 ], the adaptation of this method in built environments has become an emerging area of research. The Bacillus species used in probiotic cleaning products are also often associated with plants and soil [ 77 ].…”

Section: Techniques For Investigating the Microbiome Of The Built Env...mentioning

confidence: 99%

Biocontrol in built environments to reduce pathogen exposure and infection risk

Gottel,

Hill,

Neal

et al. 2024

The ISME Journal

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The microbiome of the built environment comprises bacterial, archaeal, fungal, and viral communities associated with human-made structures. Even though most of these microbes are benign, antibiotic-resistant pathogens can colonise and emerge indoors, creating infection risk through surface transmission or inhalation. Several studies have catalogued the microbial composition and ecology in different built environment types. These have informed in vitro studies that seek to replicate the physicochemical features that promote pathogenic survival and transmission, ultimately facilitating the development and validation of intervention techniques used to reduce pathogen accumulation. Such interventions include using Bacillus-based cleaning products on surfaces or integrating bacilli into printable materials. Though this work is in its infancy, early research suggests the potential to use microbial biocontrol to reduce hospital- and home-acquired multidrug-resistant infections. Although these techniques hold promise, there is an urgent need to better understand the microbial ecology of built environments and to determine how these biocontrol solutions alter species interactions. This review covers our current understanding of microbial ecology of the built environment and proposes strategies to translate that knowledge into effective biocontrol of antibiotic-resistant pathogens.

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“…In fact, although cleanrooms must be almost microorganism-free, the same does not apply to other hospital areas that may accommodate microbial communities [88]. Opening windows to let in outdoor environmental air and introducing green plants into the hospital are simple solutions to restore microbial biodiversity [88,99] and potentially hinder opportunistic human pathogen establishment [100]. Some precise interventions for microbial restoration, instead, involve the active manipulation, also called "biocontrol", of the indoor microbiota, for example, by the application of Bacillus spores [88,101].…”

Section: A Focus On the Hospital Ecosystem: An Inhospitable Environme...mentioning

confidence: 99%

The Microbiome of the Built Environment: The Nexus for Urban Regeneration for the Cities of Tomorrow

et al. 2022

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Built environments are, for most of us, our natural habitat. In the last 50 years, the built-up area has more than doubled, with a massive biodiversity loss. The undeniable benefits of a city providing all the basic needs to a growing population showed longer-term and less obvious costs to human health: autoimmune and non-communicable diseases, as well as antimicrobial resistance, have reached unprecedented and alarming levels. Humans coevolved with microbes, and this long-lasting alliance is affected by the loss of connection with natural environments, misuse of antibiotics, and highly sanitized environments. Our aim is to direct the focus onto the microbial communities harbored by the built environments we live in. They represent the nexus for urban regeneration, which starts from a healthy environment. Planning a city means considering, in a two-fold way, the ecosystem health and the multidimensional aspects of wellbeing, including social, cultural, and aesthetic values. The significance of this perspective is inspiring guidelines and strategies for the urban regeneration of the cities of tomorrow, exploiting the invaluable role of microbial biodiversity and the ecosystem services that it could provide to create the robust scientific knowledge that is necessary for a bioinformed design of buildings and cities for healthy and sustainable living.

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Phyllosphere-associated microbiota in built environment: Do they have the potential to antagonize human pathogens?

Cited by 13 publications

References 93 publications

The mature phyllosphere microbiome of grapevine is associated with resistance against Plasmopara viticola

The mature phyllosphere microbiome of grapevine is associated with resistance against Plasmopara viticola

Biocontrol in built environments to reduce pathogen exposure and infection risk

The Microbiome of the Built Environment: The Nexus for Urban Regeneration for the Cities of Tomorrow

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