Phyllobacterium sophorae sp. nov., a symbiotic bacterium isolated from root nodules of Sophora flavescens

Abstract

Phyllobacterium sophorae sp. nov., a symbiotic bacterium isolated from root nodules of Sophora flavescens Two novel Gram-stain-negative strains (CCBAU 03422 T and CCBAU 03415) isolated from root nodules of Sophora flavescens were classified phylogenetically into the genus Phyllobacterium based on the comparative analysis of 16S rRNA and atpD genes. They showed 99.8 % rRNA gene sequence similarities to Phyllobacterium brassicacearum LMG 22836 T , and strain CCBAU 03422 T showed 91.2 and 88.6 % atpD gene sequence similarities to strains Phyllobacterium endophyticum LMG 26470 T and Phyllobacterium brassicacearum LMG 22836 T , respectively. Strain CCBAU 03422 T contained Q-10 as its major quinone and showed a cellular fatty acid profile, carbon source utilization and other phenotypic characteristics differing from type strains of related species. DNA-DNA relatedness (lower than 48.8 %) further confirmed the differences between the novel strains and the type strains of related species. Strain CCBAU 03422 T could nodulate and fix nitrogen effectively on its original host plant, Sophora flavescens. Based upon the results mentioned above, a novel species named Phyllobacterium sophorae is proposed and the type strain is CCBAU 03422 T (5A-6-3 T 5LMG 27899 T 5HAMBI 3508 T ).The genus Phyllobacterium was originally proposed by Knösel (1962) Table 1). It is obvious that root nodules are the preferred habitats for species of the genus Phyllobacterium more so than leaf nodules (Mergaert & Swings, 2001).In studies on genetic diversity of nodule bacteria from Sophora flavescens, a legume traditionally used as herbal medicine in China, two strains (CCBAU 03422 T and CCBAU 03415) were found to be distinct from the rhizobial species in the genera Mesorhizobium, Ensifer and Rhizobium based on the sequence analysis of the recA (recombinase A)Abbreviations: DDH, DNA-DNA hybridization ; ML, maximum-likelihood.The GenBank/EMBL/DDBJ accession number for the 16S rRNA, atpD, nodC and nifH gene sequences of strain CCBAU 03422 T are KJ685937, KJ685941, KJ685943 and KJ685942, respectively.

Gastric microbial community profiling reveals a dysbiotic cancer-associated microbiota

“… 39 40 Phyllobacterium , which are environmental bacteria commonly found in plant roots, were too identified at higher abundance in gastric carcinoma. 41 All genera significantly overabundant in gastric carcinoma were also significantly more prevalent in gastric carcinoma cases than in chronic gastritis control patients, and these associations remained significant after adjustment for age and sex. In line with our results, in a study that combined terminal restriction fragment length polymorphism with 16S rRNA gene cloning and sequencing, Lactobacillus was one of the dominating genera in 10 Swedish patients with gastric cancer.…”

Diverse Bacteria Affiliated with the Genera Microvirga, Phyllobacterium, and Bradyrhizobium Nodulate Lupinus micranthus Growing in Soils of Northern Tunisia

“…(iv) Finally, the new symbiovar A, formed by Microvirga and Phyllobacterium strains, displayed similarities to Phyllobacterium strains isolated from root nodules of Sophora flavescens grown in Changzhi County in northern China (26) and with Mesorhizobium and Bradyrhizobium strains from root nodules of Caragana spp. from arid and semiarid alkaline deserts, also in northern China (54).…”

“…Phyllobacterium strains have been identified in different environments (31), mostly as plant-associated bacteria (28,30,47,48). To date, the Phyllobacterium genus contains 10 species, but only two, P. trifolii and P. sophorae, are able to induce effective nodules on their host legumes, Trifolium pratense and Sophora flavescens, respectively (26,27). P. trifolii was also reported to induce white inefficient nodules in Lupinus albus (27).…”

Shifts Between and Among Populations of Wheat Rhizosphere Pseudomonas, Streptomyces and Phyllobacterium Suggest Consistent Phosphate Mobilization at Different Wheat Growth Stages Under Abiotic Stress

“…Our study now provides evidence for the potential of two Phyllobacterium species, namely P. ifriqiyense and P. sophorae, to in vitro solubilize P from its bound mineral form in the rhizosphere of winter wheat in the temperate climatic zone. The two strains have first been isolated from root nodules of Astragalus algerianus and Lathyrus numidicus in South Tunisia -P. ifriqiyense - (Mantelin et al, 2006), and of Sophora flavescens in China -P. sophorae - (Jiao et al, 2015). Only two further previous studies indicate the potential of Phyllobacterium myrsinacearum for P solubilization in subtropical soils (Chen et al, 2006) and in metal-polluted soils (Ma et al, 2013).…”