Phycoerythrin fluorescence-based assay for peroxy radicals: A screen for biologically relevant protective agents

DeLange, Robert J.; Glazer, Alexander N.

doi:10.1016/0003-2697(89)90056-0

Cited by 164 publications

(75 citation statements)

References 21 publications

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“…In previous studies, three methods were used to assess total antioxidant capacity in dietary plants: the 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox) equivalent antioxidant capacity (TEAC) assay of Miller et al (1996), the ferric reducing ability of plasma (FRAP) assay of Benzie & Strain (1996), and the oxygen radical absorbance capacity (ORAC) assay of Glazer's laboratory (DeLange et al 1989) and others (Cao et al 1993). The TEAC and the ORAC assay are based on the antioxidant's ability to react with or neutralize free radicals generated in the assay systems, while the FRAP assay measures the reduction of Fe 3þ (ferric iron) to Fe 2þ (ferrous iron) in the presence of antioxidants.…”

Section: A Total Antioxidant Food Tablementioning

confidence: 99%

Health benefits of nuts: potential role of antioxidants

et al. 2006

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A diet rich in fruits, vegetables and minimally refined cereals is associated with lower risk for chronic degenerative diseases. Since oxidative stress is common in chronic degenerative disease, it has been assumed that dietary antioxidants may explain this protective effect. Every dietary plant contains numerous types of antioxidants with different properties. Many of these antioxidants cooperate in oxidative stress reduction in plants, and we hypothesize that many different antioxidants may also be needed for the proper protection of animal cells. To test this hypothesis, it is useful to identify dietary plants with high total antioxidant content. Several nuts are among the dietary plants with the highest content of total antioxidants. Of the tree nuts, walnuts, pecans and chestnuts have the highest contents of antioxidants. Walnuts contain more than 20 mmol antioxidants per 100 g, mostly in the walnut pellicles. Peanuts (a legume) also contribute significantly to dietary intake of antioxidants. These data are in accordance with our present extended analysis of an earlier report on nut intake and death attributed to various diseases in the Iowa Women's Health Study. We observed that the hazard ratio for total death rates showed a U-shaped association with nut/peanut butter consumption. Hazard ratio was 0·89 (CI ¼ 0·81 -0·97) and 0·81 (CI ¼ 0·75 -0·88) for nut/peanut butter intake once per week and 1-4 times per week, respectively. Death attributed to cardiovascular and coronary heart diseases showed strong and consistent reductions with increasing nut/peanut butter consumption. Further studies are needed to clarify whether antioxidants contribute to this apparent beneficial health effect of nuts. Nuts: Antioxidants: LDL oxidation: Flavonoids: FRAP: Antioxidant defence: Oxidative damageNuts are not easily defined in a manner that would be both compatible with popular usage and acceptable to botanists. For example, the groundnut or peanut is a legume, and the chufa nut, which is common in south Europe and Africa, is a tuber. Some languages such as French, even lack an umbrella word equivalent to nuts. 'Noix' in French looks like one, but just means walnuts. In this text we will not only include botanically defined nuts, but will also include data on some foods which have traditionally been defined as nuts.Nuts are highly nutritious and of prime importance for people in several regions in Asia and Africa. Most nuts contain a great deal of fat (e.g. pecan 70 %, macadamia nut 66 %, Brazil nut 65 %, walnut 60 %, almonds 55 % and peanut butter 55 %). Most have a good protein content (in the 10 -30 % range), and only a few have a very high starch content (Davidson, 1999). Recently, many nuts have also been identified as especially rich in antioxidants (Halvorsen et al. 2002;Wu et al. 2004). Nuts therefore constitute one of the most nutritionally concentrated kinds of food available. Most nuts, left in their shell, have a remarkably long shelf-life and can conveniently be stored for winter use.The aim of this paper is ...

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Section: A Total Antioxidant Food Tablementioning

confidence: 99%

Health benefits of nuts: potential role of antioxidants

et al. 2006

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“…This also examines the antioxidant capacity of all the antioxidants present in the test. 23,30 ORAC results revealed that ethanol extract of B.scandens as potent antioxidant than other extracts, indicating the direct measure of antioxidant ability of the extract. As the value of ORAC increases, antioxidant competence also increases and vice versa.…”

Section: Discussionmentioning

confidence: 95%

In vitro Antioxidant and Hepatoprotective Activity of Bridelia scandens (Roxb.)Willd

Jinadatta¹,

Rao²,

Rajshekarappa³

et al. 2017

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Natural products are emerging out as potent and alternative therapies for many diseases. Today herbs have become the part of mankind, because of its manifold ways in targeting diseased cells with minimal effects on normal cells and tissues. The present research investigated the in vitro antioxidant activity and hepatoprotective of B.scandens leaf. Preliminary phytochemical analysis exhibited the presence of most of the constituent in ethanol extract (BSE). Antioxidant capacity of various extracts of B.scandens was examined. DPPH assay revealed that ethanol extract has a good antioxidant with IC 50 value of 31.68µg/ml, whereas standard ascorbic acid with 8.78 µg/ml. BSE revealed dose dependent response with increase in concentration for reducing power assay. ORAC assay directly measured the scavenging capacity and BSE (2485 trolox eq/gm) was found to be potent than other extracts. In vitro hepatoprotective activity was performed for BSE using MTT assay in BRL 3A cell line, which revealed nontoxic dose with CTC 50 value more than 1000 µg/ml. At the dose 200 µg/ml, BSE and standard silymarin offered cell protection of 57% and 76 % respectively. Present study concludes that B.scandens leaf extract possess antioxidant potential and protect the liver cells against CCl 4 damage. However in vivo studies are being carried out to validate the traditional usage of Bridelia scandens.

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“…Trolox [6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid], a water soluble vitamin E analog serves as a positive control inhibiting fluorescein decay in a dose dependent manner (DeLang, Glazer, 1989;Cao et al, 1993).…”

Section: Oxygen Radical Absorbance Capacity Assaymentioning

confidence: 99%

Comparative antioxidant and bioavailability studies of Vitamin C in Phyllanthus emblica Linn. and its combinations with Piper nigrum Linn. and Zingiber officinale Roscoe

Somasekhar

Ashok

Kameswari

et al. 2016

Braz. J. Pharm. Sci.

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Phyllanthus emblica Linn. (amla) is used in Ayurveda, the ancient Indian system of medicine and its major constituent is vitamin C which has effective free radical scavenging property. The purpose of this study was to evaluate the in vitro antioxidant activity and the bioavailability profile of vitamin C in amla and its combinations with piperine and ginger in comparison to synthetic vitamin C using New Zealand rabbits. In vitro antioxidant activity studies of synthetic vitamin C, amla, amla with piperine and amla with ginger were carried out using different models such as 2,2-Diphenyl-1-picrylhydrazyl, Nitric Oxide, Hydrogen peroxide scavenging methods, Total reductive capability and Oxygen Radical Absorbance Capacity estimation. The study results showed that synthetic vitamin C, amla, amla with piperine and amla with ginger possess significant in vitro antioxidant activity. For bioavailability studies, synthetic vitamin C, amla, amla with piperine and amla with ginger 100 mg/kg, were administered orally and the serum samples were analyzed by HPLC at 0, 1, 2, 3, 4, 6, 8, 10, 12 and 24 hours. Bioavailability studies revealed that amla with piperine combination has higher concentration of vitamin C when compared to synthetic vitamin C. This is probably due to presence of piperine, which is a bioavailability enhancer. The present study supports the fact that amla with piperine combination can be an alternative to synthetic vitamin C.Uniterms: Phyllanthus emblica Linn. Vitamin C/antioxidant activity/in vitro study. Vitamin C/ bioavailability. Vitamin C/synthetic/antioxidant activity. Ginger. Piperine.Phyllanthus emblica Linn. (amla) é utilizada na medicina Ayurveda, medicina da Índia antiga e seu principal constituinte é a vitamina C, que possui propriedade sequestrante de radicais livres. O propósito deste estudo foi avaliar a atividade antioxidante in vitro e o perfil de biodisponibilidade da vitamina C na amla e suas combinações com piperina e gengibre em comparação com a vitamina C sintética, utilizando coelhos da Nova Zelândia. Os estudos de atividade antioxidante in vitro de vitamina C sintética, amla, amla com piperina e amla com gengibre foram realizados utilizando-se diferentes modelos para sequestrantes, como 2,2-difenil-1-picrilidrazil, óxido nítrico, peróxido de hidrogênio, capacidade redutiva total e a estimativa da capacidade de absorvância do radical oxigênio. Os resultados do estudo mostraram que vitamina C sintética, amla, amla com piperina e amla com gengibre possuem atividade antioxidante in vitro significativa. Para os estudos de biodisponibilidade, administraram-se oralmente vitamina C sintética, amla, amla com piperina e amla com gengibre 100 mg/kg e as amostras de soro foram analisadas por CLAE em 0, 1, 2, 3, 4, 6, 8, 10, 12 e 24 horas. Os estudos de biodisponibilidade revelaram que a associação de amla com piperina tem maior concentração de vitamina C, quando comparada com a vitamina C sintética. Este efeito é provavelmente devido à presença de piperina, que é intensificador de biodisponibil...

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Phycoerythrin fluorescence-based assay for peroxy radicals: A screen for biologically relevant protective agents

Cited by 164 publications

References 21 publications

Health benefits of nuts: potential role of antioxidants

Health benefits of nuts: potential role of antioxidants

In vitro Antioxidant and Hepatoprotective Activity of Bridelia scandens (Roxb.)Willd

Comparative antioxidant and bioavailability studies of Vitamin C in Phyllanthus emblica Linn. and its combinations with Piper nigrum Linn. and Zingiber officinale Roscoe

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