Phycocyanin liposomes for topical anti-inflammatory activity: in-vitro in-vivo studies

Manconi, Maria; Pendás, Jehzabel; Ledón, Nurys; Moreira, Tomás Antônio; Sinico, Chiara; Saso, Luciano; Fadda, Anna Maria

doi:10.1211/jpp.61.04.0002

Cited by 58 publications

(33 citation statements)

References 40 publications

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“…In addition, liposomes were highly biocompatible, as demonstrated by the in vitro studies that were carried out while using human keratinocytes. The ability of liposomes to improve the delivery of small and large molecules to the skin is well known [38,39]. Recently, they have been successfully proposed for the skin delivery of phytochemicals due to their high loading and carrier capabilities, as well as affinity with the skin [40].…”

Section: Discussionmentioning

confidence: 99%

Comparison between Citral and Pompia Essential Oil Loaded in Phospholipid Vesicles for the Treatment of Skin and Mucosal Infections

et al. 2020

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Citrus species extracts are well known sources of bio-functional compounds with health-promoting effects. In particular, essential oils are known for their antibacterial activity due to the high content of terpenes. In this work, the steam-distilled essential oil from the leaves of Citrus limon var. pompia was loaded in phospholipid vesicles. The physico-chemical characteristics of the essential oil loaded vesicles were compared with those of vesicles that were loaded with citral, which is one of the most abundant terpenes of Citrus essential oils. The biocompatibility of the vesicles was assessed in vitro in human keratinocytes. Furthermore, the antimicrobial activity of the vesicles was tested while using different bacterial strains and a yeast: Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Candida albicans, respectively. The vesicles were small in size (~140 nm), slightly polydispersed (PI~0.31), highly negatively charged (~−73 mV), and able to incorporate high amounts of essential oil or citral (E%~86%). Pompia essential oil and citral exhibited antimicrobial activity against all of the assayed microorganisms, with P. aeruginosa being the least sensitive. Citral was slightly more effective than pompia essential oil against E. coli, S. aureus, and C. albicans. The incorporation of citral in vesicles improved its antifungal activity against C. albicans.

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Section: Discussionmentioning

confidence: 99%

Comparison between Citral and Pompia Essential Oil Loaded in Phospholipid Vesicles for the Treatment of Skin and Mucosal Infections

et al. 2020

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“…C-phycocyanin (Cpc), a major type of phycocyanin of phycobilisome in spirulina, has been suggested to exhibit radical-scavenging property [1] to reduce inflammatory responses [2,3] and oxidative stress [1,4]. This phycobiliprotein also induces HeLa cell apoptosis [5,6] enhances wound healing [7], retards platelet aggregation [8,9] and acts as a photodynamic agent to eradicate cancer cells in vitro [10,11].…”

Section: Introductionmentioning

confidence: 99%

Antimelanogenic effect of c-phycocyanin through modulation of tyrosinase expression by upregulation of ERK and downregulation of p38 MAPK signaling pathways

Lin

Yang

et al. 2011

J Biomed Sci

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BackgroundPigmentation is one of the essential defense mechanisms against oxidative stress or UV irradiation; however, abnormal hyperpigmentation in human skin may pose a serious aesthetic problem. C-phycocyanin (Cpc) is a phycobiliprotein from spirulina and functions as an antioxidant and a light harvesting protein. Though it is known that spirulina has been used to reduce hyperpigmentation, little literature addresses the antimelanogenic mechanism of Cpc. Herein, we investigated the rationale for the Cpc-induced inhibitory mechanism on melanin synthesis in B16F10 melanoma cells.MethodsCpc-induced inhibitory effects on melanin synthesis and tyrosinase expression were evaluated. The activity of MAPK pathways-associated molecules such as MAPK/ERK and p38 MAPK, were also examined to explore Cpc-induced antimelanogenic mechanisms. Additionally, the intracellular localization of Cpc was investigated by confocal microscopic analysis to observe the migration of Cpc.ResultsCpc significantly (P < 0.05) reduced both tyrosinase activity and melanin production in a dose-dependent manner. This phycobiliprotein elevated the abundance of intracellular cAMP leading to the promotion of downstream ERK1/2 phosphorylation and the subsequent MITF (the transcription factor of tyrosinase) degradation. Further, Cpc also suppressed the activation of p38 causing the consequent disturbed activation of CREB (the transcription factor of MITF). As a result, Cpc negatively regulated tyrosinase gene expression resulting in the suppression of melanin synthesis. Moreover, the entry of Cpc into B16F10 cells was revealed by confocal immunofluorescence localization and immunoblot analysis.ConclusionsCpc exerted dual antimelanogenic mechanisms by upregulation of MAPK/ERK-dependent degradation of MITF and downregulation of p38 MAPK-regulated CREB activation to modulate melanin formation. Cpc may have potential applications in biomedicine, food, and cosmetic industries.

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“…An increase in topical bio-availability of dermatological gents has been reported for liposomes containing gel-forming polymers [61,64]. Although two decades have passed since liposomes were first postulated as drug carriers, there is only one marketed topical liposomal drug formulation, Pevaryl lipogel produced by Cilag AG (now Johnson and Johnson) which became available in Switzerland in 1988.…”

Section: Liposomesmentioning

confidence: 99%

“…Acyclovir is an antiviral used in treating viral infections of the skin and mucosa. Acyclovir is poorly absorbed across the skin due to its hydrophilic nature, and therefore not able to reach its target site in the basal epidermis, this results in reduced therapeutic efficacy [64,125]. Acyclovir is currently marketed as a cream formulation and licensed to be used topically for the treatment of herpes simplex virus (HSV), however due to its slow diffusion across the skin, it is recommended to be applied five times daily [64,126].…”

Section: Iontophoresismentioning

confidence: 99%

Topical Dermatological Drug Delivery: Quo Vadis?

Akomeah

2010

CDD

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Skin disease (dermatological conditions') affects at least one-third of the US population and has been cited as one of the top 15 medical conditions for which prevalence and healthcare spending increased in the last decade. The outcome of topical dermatological drug treatment is significantly influenced by the choice of vehicle or delivery system. Advancements in the life sciences coupled with a growing market for dermatologicals have facilitated the emergence of improved topical formulations and drug delivery systems. The current and emerging approaches of optimizing the topical delivery of dermatological agents (small and large molecules) include the use of chemical enhancers, bio-polymers (e.g. sodium hyaluronate), liposomes, particulate carriers (microspheres and lipid nanoparticles), topical sprays and foams, occlusion (via dressings and patches) topical peels, temperature (heat), iontophoresis and ultrasound. These delivery approaches (when used solely or in a synergistic manner) are a significant improvement over conventional systems (creams, lotions, ointments and pastes) and have the potential to enhance efficacy and tolerability, improve patient compliance (including dermatology life quality), and also fulfil other unmet needs of the topical dermatological market.

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Phycocyanin liposomes for topical anti-inflammatory activity: in-vitro in-vivo studies

Abstract: The results showed that C-PC can be successfully used as an anti-inflammatory drug and that liposomal encapsulation is effective in improving its anti-inflammatory activity.

Cited by 58 publications

References 40 publications

Comparison between Citral and Pompia Essential Oil Loaded in Phospholipid Vesicles for the Treatment of Skin and Mucosal Infections

Comparison between Citral and Pompia Essential Oil Loaded in Phospholipid Vesicles for the Treatment of Skin and Mucosal Infections

Antimelanogenic effect of c-phycocyanin through modulation of tyrosinase expression by upregulation of ERK and downregulation of p38 MAPK signaling pathways

Topical Dermatological Drug Delivery: Quo Vadis?

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