Phycobiliproteins Production Enhancement and Lipidomic Alteration by Titanium Dioxide Nanoparticles in Synechocystis sp. PCC 6803 Culture

Abstract

This study aimed to improve the production of phycobiliproteins using TiO nanoparticles (NPs) in Synechocystis sp. PCC 6803. The growth characteristics of Synechocystis cells were not affected by TiO NPs treatment, but this treatment increased the chlorophyll content significantly by 62.2% (14.6 mg/L) compared to that of control (9.0 mg/L) on day 16. Phycocyanin production was increased by 33.8% (29.3 g/L) compared to that of control (21.9 g/L) on day 8. Allophycocyanin production was increased by 55.0% (6.2 g/L) compared to that of control (4.0 g/L) on day 8, and by 22.4% (16.4 g/L) compared to that of control (13.4 g/L) on day 16. Direct infusion mass spectrometry revealed that TiO NPs treatment significantly increased the levels of major thylakoid membranes of monogalactosyldiacylglycerols (18:2/18:3, 18:2/18:2, 18:1/18:2), phosphatidylglycerol (16:0/16:1), and sulfoquinovosyldiacylglycerols (16:0/16:1, 16:0:18:4) on day 8. These findings indicate that TiO NPs have potential for commercial applications in Synechocystis species or other microalgal strains.

Ultrasound-Assisted Extraction and Assessment of Biological Activity of Phycobiliprotein-Rich Aqueous Extracts from Wild Cyanobacteria (Aphanizomenon flos-aquae)

“…Moreover, most of the previously performed studies focused on utilization of specific strains grown under optimal laboratory conditions. Especially for PBPs, as a result of the relatively low obtained yields, extensive and costly processing time, and upscaling limitations, alternative approaches could be considered . Interestingly, Khatoon et al, studying the effects of different media and light sources on freshwater cyanobacteria growth and PBP content, concluded that a significant cost reduction could be achieved by harvesting cyanobacteria grown in wastewater .…”

“…Especially for PBPs, as a result of the relatively low obtained yields, extensive and costly processing time, and upscaling limitations, alternative approaches could be considered. 16 Interestingly, Khatoon et al, studying the effects of different media and light sources on freshwater cyanobacteria growth and PBP content, concluded that a significant cost reduction could be achieved by harvesting cyanobacteria grown in wastewater. 17 In addition, removal of wild cyanobacteria blooms has been proposed as an important management measure, because certain cyanobacterial species are potent toxin producers and could have adverse effects on health, the environment, and local economies.…”