Phthalocyanine-mediated photodynamic therapy induces cell death and a G0/G1 cell cycle arrest in cervical cancer cells

Haywood-Small, Sarah; Vernon, David I.; Griffiths, John R.; Schofield, Jack; Brown, Stanley B.

doi:10.1016/j.bbrc.2005.11.046

Cited by 59 publications

(33 citation statements)

References 29 publications

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“…The G0/G1 cell cycle arrest was previously observed in McF-7 human breast cancer cell lines at 8 hr post-indocyanine green mediated PDT [3], and in SiHa human cervical carcinoma cells at 24 hr post-phthalocyanine-mediated PDT [15]. From these data, we concluded that PHT required a shorter time to exert its effects in vitro compared to PDT.…”

Section: Discussionsupporting

confidence: 61%

Photodynamic Hyperthermal Therapy with Indocyanine Green (ICG) induces Apoptosis and Cell Cycle Arrest in B16F10 Murine Melanoma Cells

Radzi

Osaki

Tsuka

et al. 2012

The Journal of Veterinary Medical Science

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AbSTRAcT. We examined the effects of photodynamic hyperthemal therapy (PHT), which is a combination of photodynamic therapy (PDT) and hyperthermia (HT), on the apoptosis and cell cycle progression of murine melanoma b16F10 cells. The percentage of apoptotic cell was determined by flow cytometry using fluorescein isothiocyanate (FITC)-conjugated Annexin V and propidium iodide (PI) double staining. The cell cycle analysis was performed by PI staining with flow cytometry. The expression of cyclins and heat shock protein 70 (Hsp70) were examined by a Western blotting analysis. PHT induces death in b16F10 cells, and PHT-mediated apoptosis occurred acutely and persistently in vitro. Our study demonstrated that PHT using indocyanine green (IcG) and near infrared (NIR) light source induces apoptosis and G0/G1 cell cycle arrest in the b16F10 cells.

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Section: Discussionsupporting

confidence: 61%

Photodynamic Hyperthermal Therapy with Indocyanine Green (ICG) induces Apoptosis and Cell Cycle Arrest in B16F10 Murine Melanoma Cells

Radzi

Osaki

Tsuka

et al. 2012

The Journal of Veterinary Medical Science

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“…In murine Colon26 cells, the growth of which does not appear to be influenced by gp130 stimulation via Hyper-IL-6 alone, the latter also induced prolonged growth arrest after PDT (Figure 4). Studies by others have shown that PDT disturbs cell cycle progression at the G1/S transition, which leads to growth arrest and apoptosis (Ahmad et al, 1998;Haywood-Small et al, 2006). The presence of Hyper-IL-6 enhanced and prolonged the suppression of especially Cdk2.…”

Section: Discussionmentioning

confidence: 99%

Interleukin-6 trans signalling enhances photodynamic therapy by modulating cell cycling

et al. 2007

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Photodynamic therapy (PDT) of solid tumours causes tissue damage that elicits local and systemic inflammation with major involvement of interleukin-6 (IL-6). We have previously reported that PDT-treated cells lose responsiveness to IL-6 cytokines. Therefore, it is unclear whether PDT surviving tumour cells are subject to regulation by IL-6 and whether this regulation could contribute to tumour control by PDT. We demonstrate in epithelial tumour cells that while the action of IL-6 cytokines through their membrane receptors is attenuated, regulation by IL-6 via trans-signalling is established. Soluble interleukin-6 receptor-a (IL-6Ra) (sIL-6Ra) and IL-6 were released by leucocytes in the presence of conditioned medium from PDT-treated tumour cells. Cells that had lost their membrane receptor IL-6Ra due to PDT responded to treatment with the IL-6R -IL-6 complex (Hyper-IL-6) with activation of signal transducers and activator of transcription (STAT3) and ERK. Photodynamic therapy-treated cells, which were maintained during post-PDT recovery in presence of IL-6 or Hyper-IL-6, showed an enhanced suppression of proliferation. Cytokine-dependent inhibition of proliferation correlated with a decrease in cyclin E, CDK2 and Cdc25A, and enhancement of p27kip1 and hypophosphorylated Rb. The IL-6 trans-signalling-mediated attenuation of cell proliferation was also effective in vivo detectable by an improved Colon26 tumour cure by PDT combined with Hyper-IL-6 treatment. Prevention of IL-6 trans-signalling using soluble gp130 reduced curability. The data suggest that the post-PDT tumour milieu contains the necessary components to establish effective IL-6 trans-signalling, thus providing a means for more effective tumour control.

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“…The perturbation of cell cycle appeared at 24 h post-PDT. An appreciable accumulation of cells residing in G0/G1 phase was induced at 1-2 mM, the reverse results were seen in the S and G2/M phase, as described (24). Almost all cells were destroyed when ALA 2 mM, effective light dose 10 J/cm 2 and 4 h post-PDT, which are the most optimal parameters.…”

Section: Discussionmentioning

confidence: 66%

A study on the mechanism of 5-aminolevulinic acid photodynamic therapy in vitro and in vivo in cervical cancer

Bian¹

2009

Oncol Rep

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Abstract. 5-Aminolevulinic acid-mediated photodynamic therapy (ALA-PDT) is a new ablation treatment for tumors, while its function mechanism in cervical cancer has not been elucidated. In this study, we investigated the effects of ALA-PDT on the cervical cancer cell line Me180, to search for optimal parameters of PDT. ALA-PDT on proliferation of Me180 was examined by MTT assay to find the optimal function parameters of ALA-PDT. Apoptosis was observed by using AnnexinV-FITC/PI double staining, Hoechst 33342 staining and May-Grünwald-Farbstoff Giemsa staining. Furthermore, we established a tumor model and 6 mice of each group underwent measurement of the tumor size on days 3, 7, 14, and 21 after treatment. The mRNA expression of survivin, bcl-2, p53, bax and bad in Me180 cells were detected by real-time fluorescence reverse transcriptionpolymerase chain reaction (RT-PCR) in vitro and in vivo. Finally, we compared the effects between topical and intravenous administration. Based on the above studies, we found ALA-PDT induced apoptosis and G0-G1 phase arrest of Me180 cells. The tumor volume of the topical administration and PDT group was the smallest at 7-14 days post-PDT. H&E staining showed remarkable subcutaneous necrosis in the PDT groups. The mRNA expression of survivin and bcl-2 in Me180 cells were suppressed post-PDT. Topical administration of PDT is recommended in treating cervical cancer so as to minimize the side-effects and inconvenience of phototoxic reaction brought by PDT. Our data may contribute to making the mechanism of PDT on cervical cancer clearer and give some useful suggestions for clinical application.

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Phthalocyanine-mediated photodynamic therapy induces cell death and a G0/G1 cell cycle arrest in cervical cancer cells

Cited by 59 publications

References 29 publications

Photodynamic Hyperthermal Therapy with Indocyanine Green (ICG) induces Apoptosis and Cell Cycle Arrest in B16F10 Murine Melanoma Cells

Photodynamic Hyperthermal Therapy with Indocyanine Green (ICG) induces Apoptosis and Cell Cycle Arrest in B16F10 Murine Melanoma Cells

Interleukin-6 trans signalling enhances photodynamic therapy by modulating cell cycling

A study on the mechanism of 5-aminolevulinic acid photodynamic therapy in vitro and in vivo in cervical cancer

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