Phthalate acid esters contribute to the cytotoxicity of mask leachate: Cell-based assay for toxicity assessment

Chang, Xinyi; Wang, Wen‐Xiong

doi:10.1016/j.jhazmat.2023.132093

Cited by 4 publications

(2 citation statements)

References 85 publications

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“…DEHP, as an emerging agricultural organic pollutant in environmental science, can lead to accumulation in the soil, which is absorbed by plants and enters into the food chain cycle. 45 In recent years, several studies have described that DEHP has neurotoxic and neurobehavioral toxicity, but the concrete mechanism and its association with mitochondria or ER are not clear. To address this issue, we tested whether the dysfunctional mitochondria and/or ER are involved in DEHPinduced neurotoxicity.…”

Section: Discussionmentioning

confidence: 99%

Phthalates Induce Neurotoxicity by Disrupting the Mfn2-PERK Axis-Mediated Endoplasmic Reticulum–Mitochondria Interaction

Zhao,

Chang,

Ren

et al. 2024

J. Agric. Food Chem.

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phthalate (DEHP), as the most common phthalate, has been extensively used as a plasticizer to improve the plasticity of agricultural products, which pose severe harm to human health. Mitochondrial dynamics and endoplasmic reticulum (ER) homeostasis are indispensable for maintaining mitochondria-associated ER membrane (MAM) integrity. In this study, we aimed to explore the effect of DEHP on the nervous system and its association with the ER−mitochondria interaction. Here, we showed that DEHP caused morphological changes, motor deficits, cognitive impairments, and blood−brain barrier disruption in the brain. DEHP triggered ER stress, which is mainly mediated by protein kinase R-like endoplasmic reticulum kinase (PERK) signaling. Moreover, DEHP-induced mitofusin-2 (Mfn2) downregulation results in imbalance of the mitochondrial dynamics. Interestingly, DEHP exposure impaired MAMs by inhibiting the Mfn2-PERK interaction. Above all, this study elucidates the disruption of the Mfn2-PERK axis-mediated ER−mitochondria interaction as a phthalate-induced neurotoxicity that could be potentially developed as a novel therapy for neurological diseases.

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Section: Discussionmentioning

confidence: 99%

Phthalates Induce Neurotoxicity by Disrupting the Mfn2-PERK Axis-Mediated Endoplasmic Reticulum–Mitochondria Interaction

Zhao,

Chang,

Ren

et al. 2024

J. Agric. Food Chem.

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“…A concentration of 0.5 g•L -1 of weathered face masks showed cellular disturbances leading to cytotoxicity and cell death. Sendra et al addressed that after a 3-h exposure of M. galloprovincialis haemocytes to PS MPs (1 µm -10 mg•L -1 ), a subpopulation of large granular cells exhibited significant cytotoxicity compared to the control group [78] . Additionally, these cells reached values higher than 58% of apoptotic cells when individually exposed to PS NPs of 50 and 100 nm at 10 mg•L -1 .…”

Section: In Vitro Cell Viabilitymentioning

confidence: 99%

Impact of face masks weathering on the mussels Mytilus galloprovincialis

Fonseca,

Edo,

Vilke

et al. 2023

Water Emerg Contam Nanoplastics

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The COVID-19 pandemic has triggered an unprecedented need for single-use face masks, leading to an alarming increase in plastic waste globally. Consequently, the improper disposal of face masks has added to the existing burden of plastic pollution in the oceans. However, the complete environmental and marine ecotoxicological impact remains unclear. This study aims to investigate the ecotoxicological impact caused by the weathering of disposable face masks (DFMs) in the marine environment on mussels Mytilus galloprovincialis (M. galloprovincialis ) by assessing biochemical, cytotoxic, and genotoxic effects. The mask leachate was analysed for the presence of nano and microplastics. Furthermore, the leachate was used in in vivo and in vitro toxicity bioassays to assess its impacts on M. galloprovincialis . The in vivo exposure of M. galloprovincialis to face mask leachate for 14 days induced a significant increase in catalase (CAT) activity in mussel gills, although not enough to prevent oxidative damage to cell membranes. DNA damage was also registered in mussel haemocytes after in vivo exposure to mask leachate. The in vitro Neutral Red (NR) cytotoxicity assay indicated that leachate concentrations ≤ 0.5 g/L-1 pose a significant risk to the health of mussel haemocytes, which seems a reliable tool for the cytotoxicity impact assessment of face masks in the marine environment. Therefore, the leachate obtained from face masks in seawater causes oxidative stress, oxidative damage, cytotoxicity, and genotoxicity in M. galloprovincialis , indicating that the plastic burden generated by DFMs in the ocean and its subsequent weathering represents a ubiquitous and invisible threat to the marine biota.

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Freshwater water quality criteria for phthalate esters and recommendations for the revision of the water quality standards

Hong,

Xiao,

Naraginti

et al. 2024

Ecotoxicology and Environmental Safety

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Phthalate acid esters contribute to the cytotoxicity of mask leachate: Cell-based assay for toxicity assessment

Cited by 4 publications

References 85 publications

Phthalates Induce Neurotoxicity by Disrupting the Mfn2-PERK Axis-Mediated Endoplasmic Reticulum–Mitochondria Interaction

Phthalates Induce Neurotoxicity by Disrupting the Mfn2-PERK Axis-Mediated Endoplasmic Reticulum–Mitochondria Interaction

Impact of face masks weathering on the mussels Mytilus galloprovincialis

Freshwater water quality criteria for phthalate esters and recommendations for the revision of the water quality standards

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