Phototunable interpenetrating polymer network hydrogels to stimulate the vasculogenesis of stem cell-derived endothelial progenitors

Crosby, Cody; Hillsley, Alexander; Kumar, Sachin; Stern, Brett; Parekh, Sapun H.; Rosales, Adrianne M.; Zoldan, Janet

doi:10.1016/j.actbio.2020.12.041

Cited by 17 publications

(24 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nevertheless, the absence of the vasculature can be even more severe limitation, especially for the reconstruction of the prolonged defects. One of the possibilities to fabricate vessels within a construct is to induce angiogenesis using special hydrogel-based systems (e.g., PEG–fibrin conjugates [ 48 ], norbornene-modified hyaluronic acid [ 49 ], PEG hydrogels with proteolytic specificity to matrix metalloproteinases and plasmin [ 50 ], etc. ), growth factors (VEGF, FGF, etc.…”

Section: Discussionmentioning

confidence: 99%

A defined road to tracheal reconstruction: laser structuring and cell support for rapid clinic translation

et al. 2022

View full text Add to dashboard Cite

One of the severe complications occurring because of the patient’s intubation is tracheal stenosis. Its incidence has significantly risen because of the COVID-19 pandemic and tends only to increase. Here, we propose an alternative to the donor trachea and synthetic prostheses—the tracheal equivalent. To form it, we applied the donor trachea samples, which were decellularized, cross-linked, and treated with laser to make wells on their surface, and inoculated them with human gingiva-derived mesenchymal stromal cells. The fabricated construct was assessed in vivo using nude (immunodeficient), immunosuppressed, and normal mice and rabbits. In comparison with the matrix ones, the tracheal equivalent samples demonstrated the thinning of the capsule, the significant vessel ingrowth into surrounding tissues, and the increase in the submucosa resorption. The developed construct was shown to be highly biocompatible and efficient in trachea restoration. These results can facilitate its clinical translation and be a base to design clinical trials. Graphical Abstract

show abstract

Section: Discussionmentioning

confidence: 99%

A defined road to tracheal reconstruction: laser structuring and cell support for rapid clinic translation

et al. 2022

View full text Add to dashboard Cite

show abstract

“…With the potential of differentiating into mature vascular endothelial cells, Endothelial Progenitor Cells (EPCs) participate in post-natal angiogenesis events, such as tumor angiogenesis [ 72 ] and vasculogenesis [ 73 ], within ischemic tissues [ 74 ], as well as in the maintenance of vascular homeostasis [ 75 ], in addition to embryonic vascular development [ 76 ]. Higher levels of circulating EPCs were found in MMD patients than in patients with atherosclerotic cerebrovascular disease and in healthy controls [ 77 ].…”

Section: Pathophysiologymentioning

confidence: 99%

Progression in Moyamoya Disease: Clinical Features, Neuroimaging Evaluation, and Treatment

Zhang

Xiao

Zhang

et al. 2022

View full text Add to dashboard Cite

: Moyamoya disease (MMD) is a chronic cerebrovascular disease characterized by progressive stenosis of the arteries of the circle of Willis, with the formation of the collateral vascular network at the base of the brain. Its clinical manifestations are complicated. Numerous studies have attempted to clarify the clinical features of MMD, including its epidemiology, genetic characteristics, and pathophysiology. With the development of neuroimaging techniques, various neuroimaging modalities with different advantages have deepened the understanding of MMD in structural, functional, spatial, and temporal dimensions. At present, the main treatment for MMD focuses on neurological protection, cerebral blood flow reconstruction, and neurological rehabilitation, such as pharmacological treatment, surgical revascularization, and cognitive rehabilitation. In this review, we discuss recent progress in understanding the clinical features, neuroimaging evaluation, and treatment of MMD.

show abstract

“…The CD34 + hiPSC-EP solution was then added to the collagen solution. The final concentrations for all collagen hydrogel components were described previously [10][11][12] . 56 µL of the collagen-cell suspension was pipetted into each well of an ultra-low attachment round-bottomed 96-well plate (Nexcelom) and allowed to solidify for 30 minutes at 37°C and 5% CO2.…”

Section: Encapsulation Of Cd34 + Hipsc-eps In Collagen Hydrogelsmentioning

confidence: 99%

“…Previously, our group has utilized human induced pluripotent stem cells (hiPSCs) differentiated to endothelial progenitors (EPs) and encapsulated in collagen hydrogels to study angiogenesis [10][11][12] . Here we utilize this model to demonstrate that hiPSC-EP laden hydrogels treated with SARS-CoV-2 spike protein (CSP) results in significant vascular disruption, both in terms of the number of cells part of the vascular networks as well as vascular connectivity.…”

mentioning

confidence: 99%

See 1 more Smart Citation

SARS-CoV-2 spike protein induces endothelial dysfunction in 3D engineered vascular networks

Stern

Monteleone

Zoldan

2022

Preprint

Self Cite

View full text Add to dashboard Cite

With new daily discoveries about the long-term impacts of COVID-19 there is a clear need to develop in vitro models that can be used to better understand the pathogenicity and impact of COVID-19. Here we demonstrate the utility of developing a model of endothelial dysfunction that utilizes induced pluripotent stem cell-derived endothelial progenitors encapsulated in collagen hydrogels to study the effects of COVID-19 on the endothelium. We found that treating these cell-laden hydrogels with SARS-CoV-2 spike protein resulted in a significant decrease in the number of vessel-forming cells as well as vessel network connectivity. Following treatment with the anti-inflammatory drug dexamethasone, we were able to prevent SARS-CoV-2 spike protein-induced endothelial dysfunction. In addition, we confirmed release of inflammatory cytokines associated with the COVID-19 cytokine storm. In conclusion, we have demonstrated that even in the absence of immune cells, we are able to use this 3D in vitro model for angiogenesis to reproduce COVID-19 induced endothelial dysfunction seen in clinical settings.

show abstract

Phototunable interpenetrating polymer network hydrogels to stimulate the vasculogenesis of stem cell-derived endothelial progenitors

Cited by 17 publications

References 53 publications

A defined road to tracheal reconstruction: laser structuring and cell support for rapid clinic translation

A defined road to tracheal reconstruction: laser structuring and cell support for rapid clinic translation

Progression in Moyamoya Disease: Clinical Features, Neuroimaging Evaluation, and Treatment

SARS-CoV-2 spike protein induces endothelial dysfunction in 3D engineered vascular networks

Contact Info

Product

Resources

About