Phototoxic effect of conjugates of plasmon-resonance nanoparticles with indocyanine green dye on<i>Staphylococcus aureus</i>induced by IR laser radiation

Tuchina, Elena S.; Тучин, В. В.; Khlebtsov, Boris N.; Khlebtsov, Nikolai G.

doi:10.1070/qe2011v041n04abeh014595

Cited by 29 publications

(23 citation statements)

References 44 publications

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“…Gold nanoparticles can also enhance the photoinactivation process by increasing the generation of reactive oxygen species (ROS) [58]. Tuchina et al reported on the enhanced photodynamic activity gold nanoparticlesindocyanine green dye for photoinactvation on Staphylococcus aureus [59]. Maliszewska et al reported enhanced photoinactivation of S. epidermidis by methylene blue-gold nanoparticles conjugate without any significant effect from the gold nanoparticles alone (without methylene blue) [60].…”

Section: Photoinactivation Studiesmentioning

confidence: 99%

Photoinactivation of Candida albicans and Escherichia coli using aluminium phthalocyanine on gold nanoparticles

Mthethwa

Nyokong

2015

Photochem Photobiol Sci

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The conjugates of aluminium phthalocyanine (complex 1) with gold nanorods (complex 1–AuNRs) and bipyramids (complex 1–AuBPs) showed improved singlet oxygen quantum yields of 0.23 and 0.24, respectively, compared to that of complex 1 alone at 0.12. Complex 1 and its conjugates were used for the photoinactivation of fungi (C. albicans) and bacteria cells (E. coli). The Q band absorbances were the same for the Pc alone and when conjugated to AuNPs. The efficiency of these conjugates was evaluated by measuring the log reduction of the microorganisms (C. albicans and E. coli) after irradiation with visible light in the presence of photosensitizers. Aluminium phthalocyanine alone showed log1.78 and log2.51 reductions for C. albicans and E. coli respectively. However, the conjugates showed higher photosensitization with log2.08 and log3.34 for C. albicans and E. coli, respectively using 1–AuBPs. For complex 1–AuNRs log2.53 and log3.71 were achieved for C. albicans and E. coli respectively. The statistical analysis of the results showed that the enhanced photoinactivation observed in both microorganisms was irrespective of the shape of the nanoparticles conjugated. Photoinactivation of C. albicans was less than that of E. coli even though a higher concentration of complex 1 or its conjugates was used in C. albicans.

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Section: Photoinactivation Studiesmentioning

confidence: 99%

Photoinactivation of Candida albicans and Escherichia coli using aluminium phthalocyanine on gold nanoparticles

Mthethwa

Nyokong

2015

Photochem Photobiol Sci

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“…However, due to the hydrophilic properties of 5-ALA, 5-ALA-PDT may clinically be limited by the rate of ALA uptake into the neoplastic cells and/or its penetration through the tissue. 11 Many groups found that the GNPs can enhance SOG or the photodynamic therapy efficiency of different PSs, such as phthalocyanines, 12-17 toluidine blue O,_ENREF_18 18 indocyanine green, 19,20 AlPcS 4 , 21 and hematoporphyrin. They can increase binding affinity and targeting selectivity when functionalized with multiple targeting groups, as well as tumor-selective uptake because of their size and the enhanced permeability and retention (EPR) effect.…”

Section: Introductionmentioning

confidence: 99%

Role of 5-aminolevulinic acid-conjugated gold nanoparticles for photodynamic therapy of cancer

Zhang

Wang

et al. 2015

J. Biomed. Opt

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There are three possible mechanisms for 5-aminolevulinic acid (5-ALA) conjugated gold nanoparticles (GNPs) through electrostatic bonding for photodynamic therapy (PDT) of cancer: GNPs delivery function,singlet oxygen generation (SOG) by GNPs irradiated by light, and surface resonance enhancement (SRE) of SOG. Figuring out the exact mechanism is important for further clinical treatment. 5-ALA-GNPs and human chronic myeloid leukemia K562 cells were used to study delivery function and SOG by GNPs. The SRE of SOG enabled by GNPs was explored by protoporphyrin IX (PpIX)-GNPs conjugate through electrostatic bonding.Cell experiments show that the GNPs can improve the efficiency of PDT, which is due to the vehicle effect of GNPs. PpIX–GNPs conjugate experiments demonstrated that SOG can be improved about 2.5 times over PpIX alone. The experiments and theoretical results show that the local field enhancement (LFE) via localized surface plasmon resonance (LSPR) of GNPs is the major role; the LFE was dependent on the irradiation wavelength and the GNP's size. The LFE increased with an increase of the GNP size (2R ≤ 50 nm). However, the LSPR function of the GNPs was not found in cell experiments. Our study shows that in 5-ALA-conjugated GNPs PDT, the delivery function of GNPs is the major role.

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“…Finally, we expect that the adipose tissue selective staining, for example, using a several injections of the dye directly into adipose tissue to provide lipolysis or necrosis of adipocytes without damaging of the upper layers of skin will be useful for the transfer of the presented technology to medical practice. In addition to ICG, other resonance absorbers, such as plasmonic gold nanoparticles and their conjugates with ICG, are promising . Currently, we are conducting studies to increase the selectivity of photosensitizers and enzyme lipase by their microencapsulation for prolonged release in adipose tissues .…”

Section: Discussionmentioning

confidence: 99%

Skin and subcutaneous fat morphology alterations under the LED or laser treatment in rats in vivo

Yanina

Navolokin

Bucharskaya

et al. 2019

Journal of Biophotonics

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The main objective of this work is to quantify the impact of photodynamic/photothermal treatment by using visible LED and NIR laser irradiation through the skin of subcutaneous fat in vivo followed up by tissue sampling and histology. The optical method may provide reduction of regional or site‐specific accumulations of abdominal or subcutaneous adipose tissue precisely and least‐invasively by inducing cell apoptosis and controlled necrosis of fat tissue. As photodynamic/photothermal adipose tissue sensitizers Brilliant Green (BG) or Indocyanine Green (ICG) dyes were injected subcutaneously in rats. The CW LED device (625 nm) or CW diode laser (808 nm) were used as light sources, respectively. Biopsies of skin together with subcutaneous tissues were taken for histology. The combined action BG‐staining and LED‐irradiation (BG + LED) or ICG‐staining and NIR‐laser irradiation (ICG + NIR) causes pronounced signs of damage of adipose tissue characterized by a strong stretching, thinning, folding and undulating of cell membranes and appearance of necrotic areas. As a posttreatment after 14 days only connective tissue was observed at the site of necrotic areas. The data obtained are important for safe light treatment of site‐specific fat accumulations, including cellulite. This work provides a basis for the development of fat lipolysis technologies and to move them to clinical applications. Schematics of animal experiment.

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Phototoxic effect of conjugates of plasmon-resonance nanoparticles with indocyanine green dye onStaphylococcus aureusinduced by IR laser radiation

Cited by 29 publications

References 44 publications

Photoinactivation of Candida albicans and Escherichia coli using aluminium phthalocyanine on gold nanoparticles

Photoinactivation of Candida albicans and Escherichia coli using aluminium phthalocyanine on gold nanoparticles

Role of 5-aminolevulinic acid-conjugated gold nanoparticles for photodynamic therapy of cancer

Skin and subcutaneous fat morphology alterations under the LED or laser treatment in rats in vivo

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