Photothermal Single-Particle Microscopy: Detection of a Nanolens

Selmke, Markus; Braun, Marco; Cichos, Frank

doi:10.1021/nn300181h

Cited by 143 publications

(243 citation statements)

References 36 publications

(95 reference statements)

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“…The pump beam increases the temperature, ΔT, around the focal point of the optical absorbing sample, which results in variations in the local refractive index (typically Δn ~ 10 −4 with ΔT = 1 K). Variation in the refractive index induces the deflection of the probe beam, which can be theoretically described by the Lorentz-Mie or the diffraction model [15,16]. The magnitude of the relative intensity change of the probe beam due to PT effect ΔI/I is estimated to be ~10 −4 when Δn ~ 10 −3 -10 −4 .…”

Section: Principle Of Pt Microscopymentioning

confidence: 99%

Photothermal Microscopy for High Sensitivity and High Resolution Absorption Contrast Imaging of Biological Tissues

Miyazaki

Kobayahsi

2017

Photonics

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Photothermal microscopy is useful to visualize the distribution of non-fluorescence chromoproteins in biological specimens. Here, we developed a high sensitivity and high resolution photothermal microscopy with low-cost and compact laser diodes as light sources. A new detection scheme for improving signal to noise ratio more than 4-fold is presented. It is demonstrated that spatial resolution in photothermal microscopy is up to nearly twice as high as that in the conventional widefield microscopy. Furthermore, we demonstrated the ability for distinguishing or identifying biological molecules with simultaneous muti-wavelength imaging. Simultaneous photothermal and fluorescence imaging of mouse brain tissue was conducted to visualize both neurons expressing yellow fluorescent protein and endogenous non-fluorescent chromophores.

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Section: Principle Of Pt Microscopymentioning

confidence: 99%

Photothermal Microscopy for High Sensitivity and High Resolution Absorption Contrast Imaging of Biological Tissues

Miyazaki

Kobayahsi

2017

Photonics

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“…The key advantages of PTM are its high sensitivity and no requirement of staining [16][17][18][19][20][21]). It allows for imaging of nanometer-sized absorbers buried among scatterers with high resolution and a high signal-to-noise ratio in real time [19,22,23]).…”

Section: Introductionmentioning

confidence: 99%

Label-Free Imaging of Melanoma with Confocal Photothermal Microscopy: Differentiation between Malignant and Benign Tissue

Kobayashi

Nakata

Yajima

et al. 2018

Preprint

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Label-free confocal photothermal (CPT) microscopy was utilized for the first time to investigate malignancy in mouse skin cells. A laser diode (LD) with 405nm or 488nm was used as a pump and 638nm LD as a probe for the CPT microscope. The Grey Level Cooccurrence Matrix (GLCM) for texture analysis was applied to the CPT images. Nine parameters of GLCM were calculated for the intracellular super-resolved CPT images, and the parameters Entropy and Prominence were found to be most suited among the nine parameters to discriminate between healthy cells and MM cells in case pump wavelength of 488nm is used.

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“…This results in local refractive index changes and induces the deflection of the probe beam. In the forward detection scheme, the nano-lens (thermal lens) effect produces positive and negative peaks corresponding to the focusing or defocusing of the probe beam when a sample is scanned in the axial direction [9][10][11]. This signal shape would be useful in the tracking or positioning of nanomaterials, but it causes a serious problem in biological imaging, as the twin peaks result create distortion when reconstructing an image of a structured sample.…”

Section: Introductionmentioning

confidence: 99%

Reduction of distortion in photothermal microscopy and its application to the high-resolution three-dimensional imaging of nonfluorescent tissues

Miyazaki¹,

Tsurui²,

Kobayashi³

2015

Biomed. Opt. Express

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A scheme for reducing image distortion in photothermal microscopy is presented. In photothermal microscopy, the signal shape exhibits twin peaks corresponding to the focusing or defocusing of the probe beam when a sample is scanned in the axial direction. This causes a distortion when imaging a structured sample in the axial plane. Here, we demonstrate that image distortion caused by the twin peaks is effectively suppressed by providing a small offset between two the focal planes of the pump and the probe beams. Experimental results demonstrate improvement in resolution, especially in the axial direction, over conventional optical microscopy-even with the focal offset. When a dry objective lens with a numerical aperture of 0.95 is used, the full width at half the maximum of the axial point spread function is 0.6 μm, which is 50% (62%) smaller than the focal spot sizes of the pump (probe) beam. Herein, we present highresolution three-dimensional imaging of thick biological tissues based on the present scheme.

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Photothermal Single-Particle Microscopy: Detection of a Nanolens

Cited by 143 publications

References 36 publications

Photothermal Microscopy for High Sensitivity and High Resolution Absorption Contrast Imaging of Biological Tissues

Photothermal Microscopy for High Sensitivity and High Resolution Absorption Contrast Imaging of Biological Tissues

Label-Free Imaging of Melanoma with Confocal Photothermal Microscopy: Differentiation between Malignant and Benign Tissue

Reduction of distortion in photothermal microscopy and its application to the high-resolution three-dimensional imaging of nonfluorescent tissues

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