Photothermal imaging in 3D surface analysis of membrane drug delivery

Gotter, B.; Faubel, W.; Neubert, Reinhard H.H.

doi:10.1016/j.ejpb.2009.05.014

Cited by 8 publications

(6 citation statements)

References 28 publications

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“…Molecular penetration of compounds into infected human nail plate has been investigated with the use of different approaches 12,15,16 . However, to the best of our knowledge, this is the first reported study where MALDI‐MSI is applied to assess the spatial distribution of pharmaceutical compounds in nails.…”

Section: Discussionmentioning

confidence: 99%

Visualisation of penetration of topical antifungal drug substances through mycosis‐infected nails by matrix‐assisted laser desorption ionisation mass spectrometry imaging

Pinto

Bagger

Kunze

et al. 2020

Mycoses

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Background Matrix‐assisted laser desorption ionisation mass spectrometry imaging (MALDI‐MSI) is a mass spectrometry‐based technique, which can be applied for compound‐specific imaging of pharmaceuticals in tissues samples. MALDI‐MSI technology is widely used to visualise penetration and distribution profile through different tissues but has never been used with nail tissue. Objectives This study used MALDI‐MSI technology to visualise distribution profile and penetration into ex vivo human mycosis‐infected toenails of three antifungal active ingredients amorolfine, ciclopirox and naftifine contained in topical onychomycosis nail treatment preparations, marketed as Loceryl®, Ciclopoli® and Exoderil®. Methods Three mycosis‐infected toenails were used for each treatment condition. Six and twenty‐four hours after one single topical application of antifungal drugs, excess of formulation was removed, nails were cryo‐sectioned at a thickness of 20 μm, and MALDI matrix was deposited on each nail slice. Penetration and distribution profile of amorolfine, ciclopirox and naftifine in the nails were analysed by MALDI‐MSI. Results All antifungal actives have been visualised in the nail by MALDI‐MSI. Ciclopirox and naftifine molecules showed a highly localised distribution in the uppermost layer of the nail plate. In comparison, amorolfine diffuses through the nail plate to the deep layers already 6 hours after application and keeps diffusing towards the lowest nail layers within 24 hours. Conclusions This study shows for the first‐time distribution and penetration of certain antifungal actives into human nails using MALDI‐MSI analysis. The results showed a more homogeneous distribution of amorolfine to nail and a better penetration through the infected nails than ciclopirox and naftifine.

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Section: Discussionmentioning

confidence: 99%

Visualisation of penetration of topical antifungal drug substances through mycosis‐infected nails by matrix‐assisted laser desorption ionisation mass spectrometry imaging

Pinto

Bagger

Kunze

et al. 2020

Mycoses

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“…CPO and MR showed lateral diffusion around the area of application in the in vitro transungual permeation studies. Previously, a photothermal imaging technique was reported to show the lateral distribution of the drug into the proximal end of the nail when the formulation was applied on the proximal nail fold (35).…”

Section: Discussionmentioning

confidence: 99%

“…The solution from the previous day was removed from the donor compartment, prior to application of subsequent doses of C-MR-TU. On days 5,10,15,20,25,30,35, and 40, a sample of 0.5 mL of receiver medium was removed and an equal volume of fresh pH 7.4 PBS-GS was added. Additionally on day 40, the nails were dismounted and the area of drug application and the surrounding sections of the nail periphery were cut (Fig.…”

Section: In Vitro Transungual Permeation Of Cpomentioning

confidence: 99%

Lateral Drug Diffusion in Human Nails

et al. 2014

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Abstract. The main objective of the current work is to demonstrate the process of passive lateral diffusion in the human nail plate and its effect on the passive transungual permeation of antifungal drug ciclopirox olamine (CPO). A water soluble dye, methyl red sodium salt (MR) was used to visualize the process of lateral diffusion using a novel suspended nail experiment. The decline in concentration of CPO correlates with that of concentration of MR from the proximal to the distal end of the nail in suspended nail study. Three toenails each were trimmed to 5 mm×5 mm (25 mm 2 ), 7 mm×7 mm (49 mm 2 ), and 9 mm×9 mm (81 mm 2 ) to study the extent and effect of lateral diffusion of the CPO on its in vitro transungual permeation. The permeation flux of CPO decreased as the surface area of the toenail increased. There was a positive correlation between the concentrations of CPO and MR in the area of application and in the peripheral area of the toenails of the three surface areas, confirming the findings in the suspended nail experiment. Profound lateral diffusion of CPO was demonstrated and shown to reduce the in vitro passive transungual drug permeation and prolong the lag-time in human toenails. The study data implies that during passive in vitro transungual permeation experiments, the peripheral nail around the area of drug application has to be kept to a minimum, in order to get reliable data which mimics the in vivo situation.

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“…The latter is especially important for heterogeneous or composite objects, where the classical thermophysical and thermochemical methods can give only averaged parameters of the sample. Many synthetic materials can be detected using PTS and PAS techniques with high sensitivity down to ppb level and beyond including metal nanoparticles, various dyes, and bacteria …”

Section: Outlooks For Photothermal Characterization Of Cellulose and mentioning

confidence: 99%

“…In PAS and PTS imaging and spectroscopy light absorbing contaminants and cellulose itself can serve as high contrast PT and PA agents converting absorbed energy in PT and PA signals . When samples are exposed to laser light, absorbing compounds (e. g., dyes, nanoparticles, bacteria or cells) absorb the laser energy and convert the absorbed photon through nonradiative relaxation of heat, which is detected with PTS.…”

Section: Outlooks For Photothermal Characterization Of Cellulose and mentioning

confidence: 99%

Advanced Cellulosic Materials for Treatment and Detection of Industrial Contaminants in Wastewater

et al. 2016

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The scarcity of fresh, clean water is one of the most tremendous global crises currently facing the human race. Various technologies exist for the treatment of contaminated water on different waste volume scales, for specific pollutants, and at a range of cost points. The use of agricultural and industrial by‐products directly or with simple modifications for treating wastewater has been investigated thoroughly, and the new frontier in this field is the development of advanced cellulose materials which are low cost, highly efficient, and widely applicable. Concurrently, developing highly sensitive and selective methods for analyzing the remediation efficiency of these materials is important as low concentrations of industrial contaminants can cause severe health problems in humans. The focus of this review is current progress in advanced cellulose materials for treatment of industrially‐contaminated wastewater, particularly that which contains dyes, heavy metals, and petrochemicals, and the development of ultrasensitive technologies such as photothermal spectroscopy (PTS) and photoacoustic spectroscopy (PAS) for pollutant detection.

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Photothermal imaging in 3D surface analysis of membrane drug delivery

Cited by 8 publications

References 28 publications

Visualisation of penetration of topical antifungal drug substances through mycosis‐infected nails by matrix‐assisted laser desorption ionisation mass spectrometry imaging

Visualisation of penetration of topical antifungal drug substances through mycosis‐infected nails by matrix‐assisted laser desorption ionisation mass spectrometry imaging

Lateral Drug Diffusion in Human Nails

Advanced Cellulosic Materials for Treatment and Detection of Industrial Contaminants in Wastewater

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