Photothermal detection of local thermal effects during selective nanophotothermolysis

Abstract

Photothermal (PT) technique was applied to the detection of laser-induced local thermal effects around absorbing nanoparticles into cells. The experiments demonstrated the capability of this technique for studying the threshold and the dynamic of thermal events around even a single particle in a broad temperature range, including monitoring of microbubble dynamic. PT technique shows promise for optimizing cancer-cell killing by incorporation of gold nanoparticles into the cells (selective “nanophotothermolysis”), as verified by electron microscopy and conventional viability tests.

Gold nanorod assisted near-infrared plasmonic photothermal therapy (PPTT) of squamous cell carcinoma in mice

“…Noble metal nanoparticles provide remarkable opportunities in these applications due to their inherently low toxicity [31][32][33] and strongly enhanced optical properties associated with localized surface plasmon resonance [34][35][36]. The enhanced electromagnetic field surrounding such particles gives rise to large absorption, Rayleigh (Mie) scattering, raman scattering, and twophoton luminescence cross-sections, properties which have been utilized in photothermal cancer therapy [24][25][26][27][28][29][30] (PTT), surface enhanced Raman detection [37][38][39] (SERS), and diagnostic imaging [17][18][19][20] applications.…”

“…Selectivity is achieved by focused directional control or invasive [40][41][42] (fiber optic) positioning of the incident radiation, often pulsed [28][29][30] or continuous wave [24-28, 30, 48] (cw) laser, and is typically accompanied by preferential administration of photoactive molecules [60][61][62] or nano-scale particles. Photoexcitation of the latter two results in nonradiative relaxation by local heat transfer to the surrounding tumor environment.…”

“…A variety of structures with unique structural [9,10], optical [11,12], electronic [13], magnetic [14], and catalytic [15] properties have been exploited in the areas of cancer imaging [2,[16][17][18][19], diagnostics [6,20,21], and treatment [22][23][24][25][26][27][28][29][30]. Noble metal nanoparticles provide remarkable opportunities in these applications due to their inherently low toxicity [31][32][33] and strongly enhanced optical properties associated with localized surface plasmon resonance [34][35][36].…”

Laser and Radiofrequency Induced Hyperthermia Treatment via Gold-Coated Magnetic Nanocomposites

“…In the same year, Zharov et al performed similar studies on the photothermal destruction of K562 cancer cells. They further detected the laser induced-bubbles and studied their dynamics during the treatment using a pump-probe photo thermal imaging technique (Zharov et al, 2003). O'Neal et al 2004 reported in vivo impressive results by showing selective photo-thermal ablation in mice using near infrared-absorbing NPs (O , Neal et al, 2004).…”

Direct visualization of carbon nanotube degradation in primary cells by photothermal imaging

“…11,12 Like Raman spectroscopy, PhI is an all optical method, and it is based on the detection of refractive index variations that are induced by photothermal effect in the local environment of an absorbing nanoparticle. 13,14 A few variants of PhI exist, 13,15,16 and photothermal heterodyne imaging, which is the most sensitive scheme, 13,17 allows detecting several types of nano-objects at a single particle level. These include gold nanoparticles, even within a cell matrix and for membrane receptor dynamics studies in live cells, 18,19 molecules, quantum dots or single walled carbon nanotubes.…”