Phototherapy up-regulates dentin matrix proteins expression and synthesis by stem cells from human-exfoliated deciduous teeth

Turrioni, Ana Paula Silveira; Basso, Fernanda Gonçalves; Montoro, Liege Aldrovandi; Almeida, Leopoldina de Fátima Dantas de; Costa, Carlos Alberto de Souza; Hebling, Josimeri

doi:10.1016/j.jdent.2014.07.014

Cited by 32 publications

(49 citation statements)

References 22 publications

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“…Total protein production analysis was determined by the Lowry method (16). After the extracts and pure DMEM had been aspirated from all the experimental groups and control, respectively, 100 µL 0.1% sodium lauryl phosphate, (Sigma-Aldrich Corp.) were applied in each well and the cells were incubated at room temperature for 40 min (50 µL of the cell lysis solution were used for analyzing ALP activity).…”

Section: Total Protein Productionmentioning

confidence: 99%

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Cytotoxicity of New Calcium Aluminate Cement (EndoBinder) Containing Different Radiopacifiers

et al. 2017

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This study aimed to evaluate the cytotoxicity of a calcium aluminate cement (EndoBinder) containing different radiopacifiers, Bi 2 O 3 , ZnO or ZrO 2 , compared with Mineral Trioxide Aggregate (MTA). According to ISO 10993-12:2012 (E) recommendations, 0.2 g of each cement were applied in transwell inserts and placed in 24-well culture plates containing 1 mL of culture medium (DMEM). After 24 h of incubation, the extracts (DMEM containing components released from the cements) were applied to immortalized odontoblast-like MDPC-23 cells. Cell viability (MTT test), alkaline phosphatase activity (ALP), total protein production and cell morphology (Scanning Electron Microscopy -SEM) were evaluated. The volume of 50 µL of extract was used to determine the chemical elements released by the cements using Energy Dispersive Spectroscopy (EDS). The following groups were established (n=6): NC -negative control (without treatment); EB -EndoBinder without radiopacifier; EBBO -EndoBinder+Bi 2 O 3 ; EBZnO -EndoBinder+ZnO; EBZrOEndoBinder+ZrO 2 and WMTA -White MTA. Data were subjected to statistical analysis (Kruskal-Wallis test, level of significance=5%). Cells exposed to the different versions of EndoBinder presented small reduction in viability, total protein production and ALP activity, with values similar to the NC and WMTA groups (p>0.05). Different elements (C, O, Na, Al, P, Si, Cl, Bi, K) released by the cements were detected in the extracts. However, the cells had no significant changes in their morphology. EndoBinder and MTA did not affect negatively the metabolism of the odontoblastic-like cells, showing it to be cytocompatible, irrespective of the used radiopacifier.

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Section: Total Protein Productionmentioning

confidence: 99%

“…Alkaline phosphatase activity was determined in accordance to the Alkaline Phosphatase Kit protocolEnd-Point Assay (Labtest Diagnóstico S.A., Lagoa Santa, MG, Brazil) (16).…”

Section: Alkaline Phosphatase (Alp) Activitymentioning

confidence: 99%

Cytotoxicity of New Calcium Aluminate Cement (EndoBinder) Containing Different Radiopacifiers

et al. 2017

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“…The fixed dose of 2 J/cm² was selected based on pilot studies (data not shown) using wavelengths selected from previous reports in the literature (8,16), while cell irradiation was performed one or three times consecutively to simulate a clinical protocol where the phototherapy would be applied before the insertion of the restorative materials. The results demonstrated that the increase in the frequency of light application had a detrimental effect on cell metabolic activity for both power densities.…”

Section: Discussionmentioning

confidence: 99%

“…This behavior suggests the application of red light as an adjuvant therapy to the pulp repair after pulp capping or restorative treatments performed in deep cavities. Although some in vitro and in vivo studies evaluated the effect of light sources on this process, the mechanism of action of light on the pulp tissues is not clear (3,8). It is known that light exerts positive effects on primary cultures derived from rodent pulp, inducing an increase in cell metabolism and proliferation, followed by greater production of mineralized tissue (3).…”

Section: Introductionmentioning

confidence: 99%

“…In the same study, a less intense inflammatory reaction was also observed in the teeth subjected to phototherapy, when compared to the non-irradiated teeth. Although studies observed encouraging results from the use of phototherapy with LED (3,8,10,11), finding reliable and reproducible parameters for the application of this technique in different therapeutic situations is still a challenge (12).…”

Section: Introductionmentioning

confidence: 99%

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Red LED Photobiomodulates the Metabolic Activity of Odontoblast-Like Cells

et al. 2016

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Phototherapy has been indicated as an adjunctive treatment for tissue repair, including the pulp tissue. However, there are no defined irradiation parameters, which is a great challenge to the clinical use of phototherapy. The aim of this study was to evaluate the effect of phototherapy with red LED on odontoblast-like MDPC-23 cells, using different parameter settings. Cells were seeded (10 4 cells/cm²), incubated for 12 h in complete DMEM and then the culture medium was replaced by DMEM supplemented with 0.5% FBS. After 12 h incubation, irradiations were performed (630±10 nm) using a LEDTable device with a 20 or 40 mW/cm² power density and 2 J/cm² energy dose. The cells were irradiated 1 or 3 times, at 1 min intervals. Non-irradiated cells served as control. The cells were evaluated for viability (MTT assay), total protein dosage (Lowry method) and number of viable cells (Trypan blue). The data (n=12 per group) were submitted to Kruskal-Wallis and Mann-Whitney tests (p=0.05). A single irradiation with 20 or 40 mW/cm² enhanced cell viability, which was negatively affected after 3 consecutive irradiations. Cells irradiated only once with 20 mW/cm² produced more proteins compared with those irradiated with 40 mW/cm². Reduction in the number of viable cells occurred only after 3 consecutive irradiations with 40 mW/cm². In conclusion, red LED was capable of biomodulating the metabolic activities of cultured MDPC-23 odontoblast-like cells. The best cell biostimulation was obtained when a single irradiation with 2 J/cm 2 energy dose and 20 mW/cm 2 power density was delivered to the pulp cells. R e d L E D P h o t o b i o m o d u l a t e s t h e M e t a b o l i c A c t i v i t

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Development and application of LED arrays for use in phototherapy research

Cooper

Milward

Gorecki

et al. 2017

Journal of Biophotonics

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Lasers/LEDs demonstrate therapeutic effects for a range of biomedical applications. However, a consensus on effective light irradiation parameters and efficient and reliable measurement techniques remain limited. The objective here is to develop, characterise and demonstrate the application of LED arrays in order to progress and improve the effectiveness and accuracy of in vitro photobiomodulation studies. 96-well plate format LED arrays (400-850 nm) were developed and characterised to accurately assess irradiance delivery to cell cultures. Human dental pulp cells (DPCs) were irradiated (3.5-142 mW/cm : 15-120 s) and the biological responses were assessed using MTT assays. Array calibration was confirmed using a range of optical and analytical techniques. Multivariate analysis of variance revealed biological responses were dependent on wavelength, exposure time and the post-exposure assay time (P < 0.05). Increased MTT asbsorbance was measured 24 h post-irradiation for 30 s exposures of 3.5 mW/cm at 470, 527, 631, 655, 680, 777, 798 and 826 nm with distinct peaks at 631 nm and 798 nm (P < 0.05). Similar wavelengths were also effective at higher irradiances (48-142 mW/cm ). LED arrays and high throughput assays provide a robust and reliable platform to rapidly identify irradiation parameters which is both time- and cost-effective. These arrrays are applicable in photobiomodulation, photodynamic therapy and other photobiomedical research.

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Phototherapy up-regulates dentin matrix proteins expression and synthesis by stem cells from human-exfoliated deciduous teeth

Cited by 32 publications

References 22 publications

Cytotoxicity of New Calcium Aluminate Cement (EndoBinder) Containing Different Radiopacifiers

Cytotoxicity of New Calcium Aluminate Cement (EndoBinder) Containing Different Radiopacifiers

Red LED Photobiomodulates the Metabolic Activity of Odontoblast-Like Cells

Development and application of LED arrays for use in phototherapy research

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