Phototherapy: Theory and practice

Kurz, Bernadett; Berneburg, Mark; Bäumler, Wolfgang; Karrer, Sigrid

doi:10.1111/ddg.15126

Cited by 6 publications

(2 citation statements)

References 103 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Inflammatory skin diseases, including atopic dermatitis and psoriasis, constitute an important clinical problem. The primary physical effective dermatology method for treating inflammatory skin diseases is phototherapy, including laser therapy, photodynamic therapy (PDT), bath-PUVA, and extracorporeal photochemotherapy [41,42]. It has been confirmed that photodynamic therapy has cytotoxic and immunomodulatory effects [43].…”

Section: Introductionmentioning

confidence: 99%

Cytotoxic and Immunomodulatory Effects of Hypericin as a Photosensitizer in Photodynamic Therapy Used on Skin Cell Cultures

Krupka-Olek,

Bożek,

Czuba

et al. 2024

Pharmaceutics

View full text Add to dashboard Cite

Determination of the hypericin–photodynamic (HY–PDT) effect on the secretion of cytokines secreted by the skin cells, may be the basis for using the immunomodulatory effect of photodynamic action in the treatment of inflammatory skin diseases. The study aimed to evaluate the cytotoxic and immunomodulatory effects of hypericin (HY) in photodynamic therapy (PDT) performed in vitro on cultures of selected skin cell lines. The study used two human cell lines, primary dermal fibroblast (HDFa) and primary epidermal keratinocytes (HEKa). The MTT test was used to define the metabolic activity of treated cells. Cell supernatants subjected to sublethal PDT were assessed to determine the interleukins: IL-2, IL-8, IL-10, IL-11, IL-19, IL-22, and metalloproteinase 1 (MMP-1). The results confirm the destructive effect of HY–PDT and the immunomodulatory effects of sublethal doses on the selected skin cells, depending on the concentration of HY and the light doses. No statistically significant differences were noted in IL-2 and IL-10 concentration after HY–PDT for HEKa and HDFa lines. After using HY–PDT, the concentration of IL-8, MMP-1, IL-22, and IL-11 significantly decreased in the HEKa line. Moreover, the concentration of IL-19 and MMP-1 significantly decreased in the HDFa line. The concentration of IL-11 in the HDFa line after using only the HY, without the light, increased but decreased after HY–PDT. Our experiment confirmed that HY–PDT has not only a cytotoxic effect but, used in sublethal doses, also presents immunomodulatory properties. These may be an advantage of HY–PDT when used in the treatment of persistent skin inflammation, connected with the release of pro-inflammatory cytokines resistant to conventional treatment methods.

show abstract

Section: Introductionmentioning

confidence: 99%

Cytotoxic and Immunomodulatory Effects of Hypericin as a Photosensitizer in Photodynamic Therapy Used on Skin Cell Cultures

Krupka-Olek,

Bożek,

Czuba

et al. 2024

Pharmaceutics

View full text Add to dashboard Cite

show abstract

“…The use of phototherapy for skin disorders has been reported [5] and is currently being applied in clinical settings [6]. Some reports suggesting the use of photoreception have also proposed that light exposure can promote wound healing (increasing the secretion of growth factors with red and green light) [7] and induce hair growth (accelerating the cell cycle with red light) [8].…”

Section: Introductionmentioning

confidence: 99%

Differential Photosensitivity of Fibroblasts Obtained from Normal Skin and Hypertrophic Scar Tissues

Kusumoto,

Akashi,

Terashi

et al. 2024

IJMS

View full text Add to dashboard Cite

It is unclear whether normal human skin tissue or abnormal scarring are photoreceptive. Therefore, this study investigated photosensitivity in normal skin tissue and hypertrophic scars. The expression of opsins, which are photoreceptor proteins, in normal dermal fibroblasts (NDFs) and hypertrophic scar fibroblasts (HSFs) was examined. After exposure to blue light (BL), changes in the expression levels of αSMA and clock-related genes, specifically PER2 and BMAL1, were examined in both fibroblast types. Opsins were expressed in both fibroblast types, with OPN3 exhibiting the highest expression levels. After peripheral circadian rhythm disruption, BL induced rhythm formation in NDFs. In contrast, although HSFs showed changes in clock-related gene expression levels, no distinct rhythm formation was observed. The expression level of αSMA was significantly higher in HSFs and decreased to the same level as that in NDFs upon BL exposure. When OPN3 knocked-down HSFs were exposed to BL, the reduction in αSMA expression was inhibited. This study showed that BL exposure directly triggers peripheral circadian synchronization in NDFs but not in HSFs. OPN3-mediated BL exposure inhibited HSFs. Although the current results did not elucidate the relationship between peripheral circadian rhythms and hypertrophic scars, they show that BL can be applied for the prevention and treatment of hypertrophic scars and keloids.

show abstract

Nonsteroidal Approaches for Atopic Dermatitis: A Clinical Update

Ch'en,

Lio

2024

Dermatitis®

View full text Add to dashboard Cite

Phototherapy: Theory and practice

Cited by 6 publications

References 103 publications

Cytotoxic and Immunomodulatory Effects of Hypericin as a Photosensitizer in Photodynamic Therapy Used on Skin Cell Cultures

Cytotoxic and Immunomodulatory Effects of Hypericin as a Photosensitizer in Photodynamic Therapy Used on Skin Cell Cultures

Differential Photosensitivity of Fibroblasts Obtained from Normal Skin and Hypertrophic Scar Tissues

Nonsteroidal Approaches for Atopic Dermatitis: A Clinical Update

Contact Info

Product

Resources

About