Photosynthetic Pigments in Marine Algae and Bacteria

Abstract

Biochemistry of Pigments in Algae and Bacteria Methods for Identification and Quantification of Pigments Ecological Distributions of Algal and Bacterial Pigments Pigments and Taxonomy CHEMTAX Analysis of Phytoplankton Community Composition

The effect of vitamin B12 on phytoplankton growth and community structure in the Gulf of Alaska

“…Five photopigments that are found exclusively in single classes of phytoplankton were examined as proxies for five major algal groups. Peridinin was used as an indicator of dinoflagellates, alloxanthin was analyzed as a proxy for cryptophytes, lutein indicated chlorophytes, zeaxanthin represented cyanobacteria, and fucoxanthin was used for diatoms (DiTullio and Geesey 2002). Whole water samples were preserved with 10% buffered formalin, flash-frozen in liquid nitrogen, and analyzed flow-cytometrically to assess picoplankton densities (Olson et al 1991).…”

Effects of increased pCO2 and temperature on the North Atlantic spring bloom. I. The phytoplankton community and biogeochemical response

“…Samples (400 to 1000 ml) for taxon-specific pigments were filtered onto GF/F filters (Whatman) under low vacuum at sea and were immediately frozen in liquid nitrogen for later HPLC analysis in the laboratory. Photosynthetic pigments were separated on an automated Hewlett Packard 1050 HPLC system using a reverse-phase Waters Symmetry C-8 column and a solvent gradient containing methanol, aqueous pyridine, acetone, and acetonitrile (Zapata et al 2000, DiTullio & Geesey 2002. A diode array detector recorded pigment spectra every 5 s over the wavelengths 350 to 600 nm and continuous chromatograms at 410, 440, and 455 nm.…”

“…A diode array detector recorded pigment spectra every 5 s over the wavelengths 350 to 600 nm and continuous chromatograms at 410, 440, and 455 nm. A HP 1046A fluorescence detector with excitation of 421 nm and emission at 666 nm (optimized for chl a) was also used to identify and quantify chl a and c. The system was calibrated by repeated injections of pigment standards isolated from a variety of unialgal cultures maintained in the laboratory (DiTullio & Geesey 2002).…”

A seasonal study of dissolved cobalt in the Ross Sea, Antarctica: micronutrient behavior, absence of scavenging, and relationships with Zn, Cd, and P

“…Phytoplankton pigment samples (0.5-2 L) were filtered (Whatman GF/F filters), flash frozen in liquid nitrogen, and stored at − 80 • C prior to analysis by HPLC as previously described (DiTullio and Geesey, 2002), with a slight modification using the Zapata method (Zapata et al, 2000). Water column samples for pigments were collected using a standard CTD rosette system at the same locations as trace-metal water sample collections.…”