Photosynthetic performance of outdoor Nannochloropsis mass cultures under a wide range of environmental conditions

Sukenik, Assaf; Beardall, John; Kromkamp, Jacco C.; Kopecký, Jiřka; Masojídek, Jiří; Bergeijk, S.A. van; Gabai, S.; Shaham, E.; Yamshon, A.

doi:10.3354/ame01309

Cited by 83 publications

(48 citation statements)

References 24 publications

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“…tubular photobioreactors) and open pond systems (e.g. raceway ponds; Chisti, 2007, Sukenik et al, 2009. Open pond systems are more economical to construct (Jorquera et al, 2010;Li et al, 2008b), but are highly vulnerable to Rodolfi et al (2009) contamination by unwanted bacteria, microalgae and predators.…”

Section: Challenges In Microalgal Biofuel Productionmentioning

confidence: 99%

Effects of bacterial communities on biofuel-producing microalgae: stimulation, inhibition and harvesting

Wang

Hill

Zheng

et al. 2014

Critical Reviews in Biotechnology

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Despite the great interest in microalgae as a potential source of biofuel to substitute for fossil fuels, little information is available on the effects of bacterial symbionts in mass algal cultivation systems. The bacterial communities associated with microalgae are a crucial factor in the process of microalgal biomass and lipid production and may stimulate or inhibit growth of biofuel-producing microalgae. In addition, we discuss here the potential use of bacteria to harvest biofuel-producing microalgae. We propose that aggregation of microalgae by bacteria to achieve 490% reductions in volume followed by centrifugation could be an economic approach for harvesting of biofuel-producing microalgae. Our aims in this review are to promote understanding of the effects of bacterial communities on microalgae and draw attention to the importance of this topic in the microalgal biofuel field.

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Section: Challenges In Microalgal Biofuel Productionmentioning

confidence: 99%

Effects of bacterial communities on biofuel-producing microalgae: stimulation, inhibition and harvesting

Wang

Hill

Zheng

et al. 2014

Critical Reviews in Biotechnology

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“…is a eustigmatophyte that is related to diatoms and brown algae (25). Like Chlorella but unlike Chlamydomonas, Nannochloropsis is a robust industrial alga that has been extensively grown in outdoor ponds and photobioreactors for aquaculture (26,27). Under nitrogen-starvation conditions, Nannochloropsis can accumulate oil exceeding 60% of its biomass on an ash-free dry weight basis (27), making it an excellent candidate for biofuel production.…”

mentioning

confidence: 99%

High-efficiency homologous recombination in the oil-producing alga Nannochloropsis sp.

Kilian¹,

Benemann²,

Niyogi³

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

409

301

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Algae have reemerged as potential next-generation feedstocks for biofuels, but strain improvement and progress in algal biology research have been limited by the lack of advanced molecular tools for most eukaryotic microalgae. Here we describe the development of an efficient transformation method for Nannochloropsis sp., a fast-growing, unicellular alga capable of accumulating large amounts of oil. Moreover, we provide additional evidence that Nannochloropsis is haploid, and we demonstrate that insertion of transformation constructs into the nuclear genome can occur by high-efficiency homologous recombination. As examples, we generated knockouts of the genes encoding nitrate reductase and nitrite reductase, resulting in strains that were unable to grow on nitrate and nitrate/nitrite, respectively. The application of homologous recombination in this industrially relevant alga has the potential to rapidly advance algal functional genomics and biotechnology.

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“…For example, the Aquatic Species Program (ASP) funded by the U.S. Department of Energy from 1978 to 1996 successfully isolated and tested considerable quantities (300 different species) of oleaginous microalgae that are potential resources for microalgal biofuel research (32). Further, different cultivation systems, including closed systems (e.g., tubular photobioreactors) and open pond systems (e.g., raceway ponds), have been designed based on two important criteria, biomass production efficiency and cost efficiency (4,37).…”

mentioning

confidence: 99%

Novel Bacterial Isolate from Permian Groundwater, Capable of Aggregating Potential Biofuel-Producing Microalga Nannochloropsis oceanica IMET1

Wang

Laughinghouse

Anderson

et al. 2012

Appl Environ Microbiol

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Increasing petroleum costs and climate change have resulted in microalgae receiving attention as potential biofuel producers. Little information is available on the diversity and functions of bacterial communities associated with biofuel-producing algae. A potential biofuel-producing microalgal strain, Nannochloropsis oceanica IMET1, was grown in Permian groundwater. Changes in the bacterial community structure at three temperatures were monitored by two culture-independent methods, and culturable bacteria were characterized. After 9 days of incubation, N. oceanica IMET1 began to aggregate and precipitate in cultures grown at 30°C, whereas cells remained uniformly distributed at 15°C and 25°C. The bacterial communities in cultures at 30°C changed markedly. Some bacteria isolated only at 30°C were tested for their potential for aggregating microalgae. A novel bacterium designated HW001 showed a remarkable ability to aggregate N. oceanica IMET1, causing microalgal cells to aggregate after 3 days of incubation, while the total lipid content of the microalgal cells was not affected. Direct interaction of HW001 and N. oceanica is necessary for aggregation. HW001 can also aggregate the microalgae N. oceanica CT-1, Tetraselmis suecica, and T. chuii as well as the cyanobacterium Synechococcus WH8007. 16S rRNA gene sequence comparisons indicated the great novelty of this strain, which exhibited only 89% sequence similarity with any previously cultured bacteria. Specific primers targeted to HW001 revealed that the strain originated from the Permian groundwater. This study of the bacterial communities associated with potential biofuel-producing microalgae addresses a little-investigated area of microalgal biofuel research and provides a novel approach to harvest biofuel-producing microalgae by using the novel bacterium strain HW001.

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Photosynthetic performance of outdoor Nannochloropsis mass cultures under a wide range of environmental conditions

Cited by 83 publications

References 24 publications

Effects of bacterial communities on biofuel-producing microalgae: stimulation, inhibition and harvesting

Effects of bacterial communities on biofuel-producing microalgae: stimulation, inhibition and harvesting

High-efficiency homologous recombination in the oil-producing alga Nannochloropsis sp.

Novel Bacterial Isolate from Permian Groundwater, Capable of Aggregating Potential Biofuel-Producing Microalga Nannochloropsis oceanica IMET1

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