Photosynthetic microorganisms for the oxygenation of advanced 3D bioprinted tissues

Ortega, Javiera S.; Corrales-Orovio, Rocío; Ralph, Peter J.; Egaña, José Tomás; Gentile, Carmine

doi:10.1016/j.actbio.2022.05.009

Cited by 16 publications

(15 citation statements)

References 128 publications

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“…As these cell types do not share a common environment in nature, their interaction is far from physiological. Nevertheless, several evolutionary examples support the notion that functional photosymbiotic relationships can be established between hetero and autotrophic systems [ 35 ]. Within this context, several groups have suggested that the induction of photosynthetic relationships could be established as a novel approach for the treatment of hypoxia-related pathological conditions [ 23 ].…”

Section: Discussionmentioning

confidence: 99%

“…It is recognized as the go-to microorganism model for studying photosynthesis as it is easy to culture and has an extensive array of molecular tools available [ 36 ]. Moreover, it does not produce exotoxins or infectious agents [ 37 ], which has propelled its use as a nutritional supplement and its application in several biomedical approaches [ 28 , 29 , 35 ]. On the other hand, human melanomas were chosen as the cellular model because they tend to develop acidic and hypoxic areas [ 38 ] that lead to increased tumor malignancy [ 39 ] and high resistance to conventional ROS induction and drug treatments [ 40 ].…”

Section: Discussionmentioning

confidence: 99%

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Towards an In Vitro 3D Model for Photosynthetic Cancer Treatment: A Study of Microalgae and Tumor Cell Interactions

Holmes

Varas

Martín

et al. 2022

IJMS

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As hypoxic tumors show resistance to several clinical treatments, photosynthetic microorganisms have been recently suggested as a promising safe alternative for oxygenating the tumor microenvironment. The relationship between organisms and the effect microalgae have on tumors is still largely unknown, evidencing the need for a simple yet representative model for studying photosynthetic tumor oxygenation in a reproducible manner. Here, we present a 3D photosynthetic tumor model composed of human melanoma cells and the microalgae Chlamydomonas reinhardtii, both seeded into a collagen scaffold, which allows for the simultaneous study of both cell types. This work focuses on the biocompatibility and cellular interactions of the two cell types, as well as the study of photosynthetic oxygenation of the tumor cells. It is shown that both cell types are biocompatible with one another at cell culture conditions and that a 10:1 ratio of microalgae to cells meets the metabolic requirement of the tumor cells, producing over twice the required amount of oxygen. This 3D tumor model provides an easy-to-use in vitro resource for analyzing the effects of photosynthetically produced oxygen on a tumor microenvironment, thus opening various potential research avenues.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Towards an In Vitro 3D Model for Photosynthetic Cancer Treatment: A Study of Microalgae and Tumor Cell Interactions

Holmes

Varas

Martín

et al. 2022

IJMS

Self Cite

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“…3 The release cycle of oxygen from different oxygen-releasing materials. 22,29,46,57,59,64,[66][67][68] wound healing. 65,89 There are many methods of curing and molding hydrogels, such as free radical polymerization crosslinking, light curing, and physical cross-linking.…”

Section: Hydrogel Wrappingmentioning

confidence: 99%

Oxygen-releasing biomaterials for regenerative medicine

Wang,

Chen,

et al. 2023

J. Mater. Chem. B

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“…With its antioxidative, antidiabetic, antibacterial, and antiinflammatory properties, this dressing holds promise as a potential treatment to accelerate the healing of DCWs. [18,19,76] The rich content and diverse species of algae extracts resemble the state of living algae when applied to wounds or the release of their contents after inactivation. The material prepared by Wu et al can inactivate the content release of oxygen consuming Chlorella at night.…”

Section: Considerable Biological Powerful Inclusionsmentioning

confidence: 99%

Recent Advances in Living Algae Seeding Wound Dressing: Focusing on Diabetic Chronic Wound Healing

Ma,

Fang,

et al. 2023

Adv Funct Materials

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Diabetic chronic wounds (DCWs) are a severe complication of diabetes, and current treatments present several deficiencies, including unsatisfactory efficacy and inability to continuously supply oxygen. This review highlights the recent advances in a novel therapeutic strategy that addresses these challenges: considerable biological powerful inclusions, oxygen or hydrogen and ROS production, DCW glucose consumption, chemotaxis at the wound site, highly editable, great biological safety, low cost, and readily available. Over the past decade, this strategy has shown encouraging results in improving the healing delay induced by hyperglycemia, forming a new concept of “living material” application based on living algae. This review discusses the unique advantages of algae as a treatment strategy, the applications and characteristics of different types of living algae in wound dressings, and the challenges and improvement strategies for this innovative approach.

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Photosynthetic microorganisms for the oxygenation of advanced 3D bioprinted tissues

Cited by 16 publications

References 128 publications

Towards an In Vitro 3D Model for Photosynthetic Cancer Treatment: A Study of Microalgae and Tumor Cell Interactions

Towards an In Vitro 3D Model for Photosynthetic Cancer Treatment: A Study of Microalgae and Tumor Cell Interactions

Oxygen-releasing biomaterials for regenerative medicine

Recent Advances in Living Algae Seeding Wound Dressing: Focusing on Diabetic Chronic Wound Healing

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