“…In addition to crtI, five putative genes (crtE, crtB, crtC, crtD and crtF) required for both spheroidene and spirilloxanthin synthesis have been found and cloned from Rvi. gelatinosus (Ouchane et al 1997b, Igarashi et al 1998, Igarashi et al 2001, Steiger et al 2000. The results of our complementation experiments suggested that these genes functioned in both pathways.…”
Section: Complementation Of Crti With Various Crti Genesmentioning
confidence: 64%
“…gelatinosus photosynthesis gene cluster cloned previously (Igarashi et al 1998, Igarashi et al 2001. The fragment was cloned in pUC118 at the BamHI and HincII restriction sites, creating a plasmid pUCCRT-I.…”
Section: Plasmidsmentioning
confidence: 99%
“…gelatinosus wild type, especially in the content of spirilloxanthin (Table 2). Since CrtI of E. uredovora catalyzes four-step desaturation of phytoene and produces lyco- (Ouchane et al 1997b, Igarashi et al 1998, Igarashi et al 2001 pene, the presence of spirilloxanthin seems reasonable. In this strain, a significant amount of OH-spheroidene was also formed from neurosporene (Table 2).…”
Section: Complementation Of Crti With Various Crti Genesmentioning
confidence: 99%
“…gelatinosus (Igarashi et al 1998, Igarashi et al 2001), a gene corresponding to crtI was found. In this study, we characterized the crtI product of this bacterium and found that the CrtI can produce neurosporene by three-step desaturation and lycopene by fourstep desaturation.…”
;Biosynthetic pathways for carotenoids in the purple photosynthetic bacterium, Rubrivivax gelatinosus, which synthesizes spirilloxanthin in addition to spheroidene and OH-spheroidene, were investigated by means of genetic manipulation. A phytoene desaturase gene (crtI) found in the photosynthesis gene cluster of this bacterium was expressed in an Escherichia coli strain that can produce phytoene. Both neurosporene and lycopene were synthesized in the recombinant, probably by three-and four-step desaturation reactions of CrtI. A mutant of Rvi. gelatinosus lacking the crtI gene produced only phytoene, indicating that this organism had no other phytoene desaturases. When the crtI deletion mutant was complemented by the three-step phytoene desaturase of Rhodobacter capsulatus, spirilloxanthin and its precursors were not synthesized, although spheroidene and OH-spheroidene were accumulated. It was concluded that neurosporene and lycopene are produced by a single phytoene desaturase in Rvi. gelatinosus resulting in the synthesis of spheroidene and spirilloxanthin, and that there are no pathways for spirilloxanthin synthesis via spheroidene.
“…In addition to crtI, five putative genes (crtE, crtB, crtC, crtD and crtF) required for both spheroidene and spirilloxanthin synthesis have been found and cloned from Rvi. gelatinosus (Ouchane et al 1997b, Igarashi et al 1998, Igarashi et al 2001, Steiger et al 2000. The results of our complementation experiments suggested that these genes functioned in both pathways.…”
Section: Complementation Of Crti With Various Crti Genesmentioning
confidence: 64%
“…gelatinosus photosynthesis gene cluster cloned previously (Igarashi et al 1998, Igarashi et al 2001. The fragment was cloned in pUC118 at the BamHI and HincII restriction sites, creating a plasmid pUCCRT-I.…”
Section: Plasmidsmentioning
confidence: 99%
“…gelatinosus wild type, especially in the content of spirilloxanthin (Table 2). Since CrtI of E. uredovora catalyzes four-step desaturation of phytoene and produces lyco- (Ouchane et al 1997b, Igarashi et al 1998, Igarashi et al 2001 pene, the presence of spirilloxanthin seems reasonable. In this strain, a significant amount of OH-spheroidene was also formed from neurosporene (Table 2).…”
Section: Complementation Of Crti With Various Crti Genesmentioning
confidence: 99%
“…gelatinosus (Igarashi et al 1998, Igarashi et al 2001), a gene corresponding to crtI was found. In this study, we characterized the crtI product of this bacterium and found that the CrtI can produce neurosporene by three-step desaturation and lycopene by fourstep desaturation.…”
;Biosynthetic pathways for carotenoids in the purple photosynthetic bacterium, Rubrivivax gelatinosus, which synthesizes spirilloxanthin in addition to spheroidene and OH-spheroidene, were investigated by means of genetic manipulation. A phytoene desaturase gene (crtI) found in the photosynthesis gene cluster of this bacterium was expressed in an Escherichia coli strain that can produce phytoene. Both neurosporene and lycopene were synthesized in the recombinant, probably by three-and four-step desaturation reactions of CrtI. A mutant of Rvi. gelatinosus lacking the crtI gene produced only phytoene, indicating that this organism had no other phytoene desaturases. When the crtI deletion mutant was complemented by the three-step phytoene desaturase of Rhodobacter capsulatus, spirilloxanthin and its precursors were not synthesized, although spheroidene and OH-spheroidene were accumulated. It was concluded that neurosporene and lycopene are produced by a single phytoene desaturase in Rvi. gelatinosus resulting in the synthesis of spheroidene and spirilloxanthin, and that there are no pathways for spirilloxanthin synthesis via spheroidene.
A 37-kb photosynthesis gene cluster was sequenced in a photosynthetic bacterium belonging to the beta subclass of purple bacteria (Proteobacteria), Rubrivivax gelatinosus. The cluster contained 12 bacteriochlorophyll biosynthesis genes (bch), 7 carotenoid biosynthesis genes (crt), structural genes for photosynthetic apparatuses (puf and puh), and some other related genes. The gene arrangement was markedly different from those of other purple photosynthetic bacteria, while two superoperonal structures, crtEF-bchCXYZ-puf and bchFNBHLM-lhaA-puhA, were conserved. Molecular phylogenetic analyses of these photosynthesis genes showed that the photosynthesis gene cluster of Rvi. gelatinosus was originated from those of the species belonging to the alpha subclass of purple bacteria. It was concluded that a horizontal transfer of the photosynthesis gene cluster from an ancestral species belonging to the alpha subclass to that of the beta subclass of purple bacteria had occurred and was followed by rearrangements of the operons in this cluster.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.